TY - JOUR
T1 - Levels of cytokines in broncho-alveolar lavage fluid, but not in plasma, are associated with levels of markers of lipid peroxidation in breath of ventilated ICU patients
AU - Boshuizen, Margit
AU - Leopold, Jan Hendrik
AU - Zakharkina, Tetyana
AU - Knobel, Hugo H.
AU - Weda, Hans
AU - Nijsen, Tamara M. E.
AU - Vink, Teunis J.
AU - Sterk, Peter J.
AU - Schultz, Marcus J.
AU - Bos, Lieuwe D. J.
AU - AUTHOR GROUP
AU - Cremer, Olaf L.
AU - Klein Klouwenberg, Peter M. C.
AU - Ong, David S. Y.
AU - Frencken, Jos F.
AU - Bonten, Marc J. M.
AU - Horn, Janneke
AU - Juffermans, Nicole
AU - van Hooijdonk, Roosmarijn T. M.
AU - Huson, Mischa A.
AU - Schouten, Laura R. A.
AU - Straat, Marleen
AU - van Vught, Lonneke A.
AU - Wiewel, Maryse A.
AU - Witteveen, Esther
AU - Glas, Gerie J.
AU - de Beer, Friso M.
AU - Wieske, Luuk
AU - van der Poll, Tom
PY - 2015
Y1 - 2015
N2 - Alkanes and alkenes in the breath are produced through fatty acid peroxidation, which is initialized by reactive oxygen species. Inflammation is an important cause and effect of reactive oxygen species. We aimed to evaluate the association between fatty acid peroxidation products and inflammation of the alveolar and systemic compartment in ventilated intensive care unit (ICU) patients.Volatile organic compounds were measured by gas chromatography and mass spectrometry in the breath of newly ventilated ICU patients within 24 h after ICU admission. Cytokines were measured in non-directed bronchial lavage fluid (NBL) and plasma by cytometric bead array. Correlation coefficients were calculated and presented in heatmaps.93 patients were included. Peroxidation products in exhaled breath were not associated with markers of inflammation in plasma, but were correlated with those in NBL. IL-6, IL-8, IL-1β and TNF-α concentration in NBL showed inverse correlation coefficients with the peroxidation products of fatty acids. Furthermore, NBL IL-10, IL-13, GM-CSF and IFNγ demonstrated positive associations with breath alkanes and alkenes. Correlation coefficients for NBL cytokines were high regarding peroxidation products of n-6, n-7 and particularly in n-9 fatty acids.Levels of lipid peroxidation products in the breath of ventilated ICU patients are associated with levels of inflammatory markers in NBL, but not in plasma. Alkanes and alkenes in breath seems to be associated with an anti-inflammatory, rather than a pro-inflammatory state in the alveoli
AB - Alkanes and alkenes in the breath are produced through fatty acid peroxidation, which is initialized by reactive oxygen species. Inflammation is an important cause and effect of reactive oxygen species. We aimed to evaluate the association between fatty acid peroxidation products and inflammation of the alveolar and systemic compartment in ventilated intensive care unit (ICU) patients.Volatile organic compounds were measured by gas chromatography and mass spectrometry in the breath of newly ventilated ICU patients within 24 h after ICU admission. Cytokines were measured in non-directed bronchial lavage fluid (NBL) and plasma by cytometric bead array. Correlation coefficients were calculated and presented in heatmaps.93 patients were included. Peroxidation products in exhaled breath were not associated with markers of inflammation in plasma, but were correlated with those in NBL. IL-6, IL-8, IL-1β and TNF-α concentration in NBL showed inverse correlation coefficients with the peroxidation products of fatty acids. Furthermore, NBL IL-10, IL-13, GM-CSF and IFNγ demonstrated positive associations with breath alkanes and alkenes. Correlation coefficients for NBL cytokines were high regarding peroxidation products of n-6, n-7 and particularly in n-9 fatty acids.Levels of lipid peroxidation products in the breath of ventilated ICU patients are associated with levels of inflammatory markers in NBL, but not in plasma. Alkanes and alkenes in breath seems to be associated with an anti-inflammatory, rather than a pro-inflammatory state in the alveoli
U2 - https://doi.org/10.1088/1752-7155/9/3/036010
DO - https://doi.org/10.1088/1752-7155/9/3/036010
M3 - Article
C2 - 26333527
SN - 1752-7155
VL - 9
SP - 036010
JO - Journal of breath research
JF - Journal of breath research
IS - 3
ER -