Abstract
In this study we tested whether HIV-1 replication could be inhibited by stable RNAi-mediated knockdown of cellular co-factors. Cell lines capable of expressing shRNAs against 30 candidate co-factors implicated at different steps of the viral replication cycle were generated and analyzed for effects on cell viability and inhibition of HIV-1 replication. For half of these candidate co-factors we obtained knockdown cell lines that are less susceptible to virus replication. For three co-factors (ALIX, ATG16 and TRBP) the cell lines were resistant to HIV-1 replication for up to 2 months. With these cells we could test the hypothesis that HIV-1 is not able to escape from RNAi-mediated suppression of cellular co-factors, which was indeed not detected.
| Original language | English |
|---|---|
| Pages (from-to) | 43-53 |
| Number of pages | 11 |
| Journal | Antiviral Research |
| Volume | 89 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 2011 |
Keywords
- Autophagy-Related Proteins
- Calcium-Binding Proteins/antagonists & inhibitors
- Carrier Proteins/antagonists & inhibitors
- Cell Cycle Proteins/antagonists & inhibitors
- Cell Line
- Cell Survival
- Endosomal Sorting Complexes Required for Transport/antagonists & inhibitors
- Gene Knockdown Techniques
- HIV-1/genetics
- Humans
- RNA Interference
- RNA, Small Interfering/genetics
- RNA-Binding Proteins/antagonists & inhibitors
- Time Factors
- Virus Replication