TY - JOUR
T1 - Longitudinal analysis on the ecological dynamics of the cervicovaginal microbiome in hrHPV infection
AU - Molina, Mariano A.
AU - Melchers, Willem J.G.
AU - Andralojc, Karolina M.
AU - Leenders, William P.J.
AU - Huynen, Martijn A.
N1 - Funding Information: This work was supported by a research grant from the Ruby and Rose Foundation . The sequence read data generated in this study are available at NCBI in the Sequencing Read Archive , projects PRJNA856437 (174 files) [69] and PRJEB45937 (108 files) [70] . Sample Accession Numbers are shown as Supplementary material. Funding Information: This work was supported by a research grant from the Ruby and Rose Foundation. The sequence read data generated in this study are available at NCBI in the Sequencing Read Archive, projects PRJNA856437 (174 files) [69] and PRJEB45937 (108 files) [70]. Sample Accession Numbers are shown as Supplementary material. Publisher Copyright: © 2023 The Authors
PY - 2023/1/1
Y1 - 2023/1/1
N2 - The cervicovaginal microbiome (CVM) is a dynamic continuous microenvironment that can be clustered in microbial community state types (CSTs) and is associated with women's cervical health. Lactobacillus-depleted communities particularly associate with an increased susceptibility for persistence of high-risk human papillomavirus (hrHPV) infections and progression of disease, but the long-term ecological dynamics of CSTs after hrHPV infection diagnosis remain poorly understood. To determine such dynamics, we examined the CVM of our longitudinal cohort of 141 women diagnosed with hrHPV infection at baseline with collected cervical smears at two timepoints six-months apart. Here we describe that the long-term microbiome dissimilarity has a positive correlation with microbial diversity at both visits and that women with high abundance and dominance for Lactobacillus iners at baseline exhibit more similar microbiome composition at second visit than women with Lactobacillus-depleted communities at baseline. We further show that the species Lactobacillus acidophilus and Megasphaera genomosp type 1 associate with CST changes between both visits. Lastly, we also observe that Gardnerella vaginalis is associated with the stability of Lactobacillus-depleted communities while L. iners is associated with the instability of Megasphaera genomosp type 1-dominated communities. Our data suggest dynamic patterns of cervicovaginal CSTs during hrHPV infection, which could be potentially used to develop microbiome-based therapies against infection progression towards disease.
AB - The cervicovaginal microbiome (CVM) is a dynamic continuous microenvironment that can be clustered in microbial community state types (CSTs) and is associated with women's cervical health. Lactobacillus-depleted communities particularly associate with an increased susceptibility for persistence of high-risk human papillomavirus (hrHPV) infections and progression of disease, but the long-term ecological dynamics of CSTs after hrHPV infection diagnosis remain poorly understood. To determine such dynamics, we examined the CVM of our longitudinal cohort of 141 women diagnosed with hrHPV infection at baseline with collected cervical smears at two timepoints six-months apart. Here we describe that the long-term microbiome dissimilarity has a positive correlation with microbial diversity at both visits and that women with high abundance and dominance for Lactobacillus iners at baseline exhibit more similar microbiome composition at second visit than women with Lactobacillus-depleted communities at baseline. We further show that the species Lactobacillus acidophilus and Megasphaera genomosp type 1 associate with CST changes between both visits. Lastly, we also observe that Gardnerella vaginalis is associated with the stability of Lactobacillus-depleted communities while L. iners is associated with the instability of Megasphaera genomosp type 1-dominated communities. Our data suggest dynamic patterns of cervicovaginal CSTs during hrHPV infection, which could be potentially used to develop microbiome-based therapies against infection progression towards disease.
KW - Cervical cancer
KW - Cervicovaginal microbiome
KW - Dynamics
KW - HrHPV
KW - Microbial communities
KW - Microbiome
UR - http://www.scopus.com/inward/record.url?scp=85171375365&partnerID=8YFLogxK
U2 - https://doi.org/10.1016/j.csbj.2023.09.011
DO - https://doi.org/10.1016/j.csbj.2023.09.011
M3 - Article
C2 - 37731597
SN - 2001-0370
VL - 21
SP - 4424
EP - 4431
JO - Computational and structural biotechnology journal
JF - Computational and structural biotechnology journal
ER -