TY - JOUR
T1 - Low serum angiopoietin-1, high serum angiopoietin-2, and high ANG-2/ANG-1 protein ratio are associated with early onset sepsis in surinamese newborns
AU - Zonneveld, Rens
AU - Jongman, Rianne
AU - Juliana, Amadu
AU - Zijlmans, Wilco
AU - Plötz, Frans
AU - Molema, Grietje
AU - van Meurs, Matijs
PY - 2017
Y1 - 2017
N2 - Purpose: Vascular inflammation and leakage in sepsis is mediated by Angiopoietin-1 (Ang-1) and Angiopoietin-2 (Ang-2) and their phosphorylation of the endothelial Tie-2 receptor. This study investigates levels of Ang-1 and Ang-2 in newborns to gain insight in the vascular pathophysiology of early onset sepsis (EOS) within 72 h after birth. Methods: A prospective cohort study was performed among 71 Surinamese newborns treated with antibiotics for suspected EOS and 20 control newborns. Newborns with suspected EOS were divided in two groups: blood culture negative and positive EOS. Ang-1 and Ang-2 levels were measured in serum obtained at the start of antibiotic treatment and at re-evaluation after 48 to 72 h. Results: In this cohort 8.5% of newborns had a positive blood culture. At the start of antibiotic treatment Ang-1 serum levels were lower (P<0.01), and Ang-2 and Ang-2/ Ang-1 serum protein ratios were higher (P<0.01 and P<0.01, respectively) in newborns with blood culture positive EOS than in controls. These levels were not dependent on timing of first blood draw after birth. After 48 to 72 h levels of Ang-1 further decreased in blood culture positive EOS, while in the other groups no change was observed. Conclusions: Our findings support the hypothesis that a disbalance in the Angiopoietins plays a role in the vascular pathophysiology of EOS.
AB - Purpose: Vascular inflammation and leakage in sepsis is mediated by Angiopoietin-1 (Ang-1) and Angiopoietin-2 (Ang-2) and their phosphorylation of the endothelial Tie-2 receptor. This study investigates levels of Ang-1 and Ang-2 in newborns to gain insight in the vascular pathophysiology of early onset sepsis (EOS) within 72 h after birth. Methods: A prospective cohort study was performed among 71 Surinamese newborns treated with antibiotics for suspected EOS and 20 control newborns. Newborns with suspected EOS were divided in two groups: blood culture negative and positive EOS. Ang-1 and Ang-2 levels were measured in serum obtained at the start of antibiotic treatment and at re-evaluation after 48 to 72 h. Results: In this cohort 8.5% of newborns had a positive blood culture. At the start of antibiotic treatment Ang-1 serum levels were lower (P<0.01), and Ang-2 and Ang-2/ Ang-1 serum protein ratios were higher (P<0.01 and P<0.01, respectively) in newborns with blood culture positive EOS than in controls. These levels were not dependent on timing of first blood draw after birth. After 48 to 72 h levels of Ang-1 further decreased in blood culture positive EOS, while in the other groups no change was observed. Conclusions: Our findings support the hypothesis that a disbalance in the Angiopoietins plays a role in the vascular pathophysiology of EOS.
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85019650368&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/28538018
U2 - https://doi.org/10.1097/SHK.0000000000000903
DO - https://doi.org/10.1097/SHK.0000000000000903
M3 - Article
C2 - 28538018
SN - 1073-2322
VL - 48
SP - 638
EP - 643
JO - Shock
JF - Shock
IS - 6
ER -