@article{dab2ccec1c194b338726942252b0d3ae,
title = "Lysosome recruitment and fusion are early events required for trypanosome invasion of mammalian cells",
abstract = "Trypanosoma cruzi invades most nucleated cells by a mechanism distinct from classical phagocytosis. Although parasites enter at the lysosome-poor peripheral cell margins, lysosomal markers are immediately incorporated into the parasitophorous vacuole. No accumulation of polymerized actin was detected around recently internalized parasites, and disruption of microfilaments significantly facilitated invasion. Lysosomes were observed to aggregate at the sites of trypanosome attachment and to fuse with the vacuole at early stages of its formation. Experimentally induced, microtubule-dependent movement of lysosomes from the perinuclear area to the cell periphery enhanced entry. Conditions that deplete cells of peripheral lysosomes or interfere with lysosomal fusion capacity inhibited invasion. These observations reveal a novel mechanism for cell invasion: recruitment of lysosomes for fusion at the site of parasite internalization.",
author = "Isabelle Tardieux and Paul Webster and Jan Ravesloot and Walter Boron and Lunn, {J. Adrian} and Heuser, {John E.} and Andrews, {Norma W.}",
note = "Funding Information: We are very grateful to I. Mellman for helpful discussions and for his generous gift of antibodies to rat Igp 120. We would also like to thank R. Montgomery for suggestions, A. K. Ma for excellent technical assistance, I. Nabi and E. Rodriguez-Boulan for providing antibodies to dog Igp, P. M#de for help with photography and confocal microscopy, and D. L. Sacks, B. F. Hall, R. Baron, C. Beckers, and G. Milon for critical reading of the manuscript. These studies were supported by grants R29AI27260 and RO1AI32056 from the National Institutes of Health (to N. W. A.). J. A. L. is the recipient of a Howard Hughes predoctoral fellowship, and N. W. A. is a Burroughs Welcome New Investigator in Molecular Parasitology.",
year = "1992",
month = dec,
day = "24",
doi = "https://doi.org/10.1016/S0092-8674(05)80061-3",
language = "English",
volume = "71",
pages = "1117--1130",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "7",
}