TY - JOUR
T1 - Measurable residual disease in acute myeloid leukemia using flow cytometry
T2 - approaches for harmonization/standardization
AU - Schuurhuis, Gerrit J.
AU - Ossenkoppele, Gert J.
AU - Kelder, Angèle
AU - Cloos, Jacqueline
PY - 2018/12/2
Y1 - 2018/12/2
N2 - Introduction: Measurable residual disease (MRD) in acute myeloid leukemia (AML) is a rapidly evolving area with many institutes embarking on it, both in academic and pharmaceutical settings. However, there is a multitude of approaches to design, perform, and report flow cytometric MRD. Together with the long-term experience needed, this makes flow cytometric MRD in AML nonstandardized and time-consuming. Areas covered: This paper briefly summarizes critical issues, like sample preparation and transport, markers and fluorochromes of choice, but in particular focuses on the main issues, which includes specificity and sensitivity, hereby providing a new model that may circumvent the main disadvantages of the present approaches. New approaches that may add to the value of flow cytometric MRD includes assessment of leukemia stem cells, MRD in peripheral blood, and approaches to use multidimensional image analysis. Expert commentary: MRD in AML requires standardization/harmonization on many aspects, for which the present paper offers possible guidelines.
AB - Introduction: Measurable residual disease (MRD) in acute myeloid leukemia (AML) is a rapidly evolving area with many institutes embarking on it, both in academic and pharmaceutical settings. However, there is a multitude of approaches to design, perform, and report flow cytometric MRD. Together with the long-term experience needed, this makes flow cytometric MRD in AML nonstandardized and time-consuming. Areas covered: This paper briefly summarizes critical issues, like sample preparation and transport, markers and fluorochromes of choice, but in particular focuses on the main issues, which includes specificity and sensitivity, hereby providing a new model that may circumvent the main disadvantages of the present approaches. New approaches that may add to the value of flow cytometric MRD includes assessment of leukemia stem cells, MRD in peripheral blood, and approaches to use multidimensional image analysis. Expert commentary: MRD in AML requires standardization/harmonization on many aspects, for which the present paper offers possible guidelines.
KW - Multicolor flow cytometry
KW - acute myeloid leukemia
KW - leukemia stem cells
KW - measurable residual disease
KW - multidimensional image analysis
KW - sensitivity
KW - specificity
KW - standardization/harmonization
UR - http://www.scopus.com/inward/record.url?scp=85058341507&partnerID=8YFLogxK
U2 - https://doi.org/10.1080/17474086.2018.1549479
DO - https://doi.org/10.1080/17474086.2018.1549479
M3 - Article
C2 - 30466339
SN - 1747-4086
VL - 11
SP - 921
EP - 935
JO - Expert review of hematology
JF - Expert review of hematology
IS - 12
ER -