MECHANISM OF THE STIMULATION OF RESPIRATION BY FATTY-ACIDS IN RAT-LIVER

P. J. Plomp; C. W. van Roermund; A. K. Groen; A. J. Meijer; J. M. Tager

doi:https://doi.org/10.1016/0014-5793(85)80161-7

MECHANISM OF THE STIMULATION OF RESPIRATION BY FATTY-ACIDS IN RAT-LIVER

P. J. Plomp, C. W. van Roermund, A. K. Groen, A. J. Meijer, J. M. Tager

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Abstract

The mechanism of stimulation of hepatic respiration by fatty acids was studied in isolated rat hepatocytes. Stimulation of respiration by fatty acids varied from about 35% to about 105% depending on chain length. The stimulatory effect of octanoate (1 mM) or oleate (0.5 mM) was prevented by oligomycin (2 micrograms/ml). With carboxyatractyloside (100 microM) and ouabain (2 mM) the stimulation of respiration was partially inhibited (by 50-70 and 50-60%, respectively). From these results it can be concluded that the increased rate of respiration after addition of fatty acids is coupled to ATP synthesis. A large part (50-60%) of this ATP is utilized by the (Na+ + K+)-ATPase

Original language	English
Pages (from-to)	243-246
Journal	FEBS letters
Volume	193
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(85)80161-7
Publication status	Published - 1985

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https://doi.org/10.1016/0014-5793(85)80161-7

Cite this

@article{9c5a774b096341a2a405c5741c8abd8c,

title = "MECHANISM OF THE STIMULATION OF RESPIRATION BY FATTY-ACIDS IN RAT-LIVER",

abstract = "The mechanism of stimulation of hepatic respiration by fatty acids was studied in isolated rat hepatocytes. Stimulation of respiration by fatty acids varied from about 35% to about 105% depending on chain length. The stimulatory effect of octanoate (1 mM) or oleate (0.5 mM) was prevented by oligomycin (2 micrograms/ml). With carboxyatractyloside (100 microM) and ouabain (2 mM) the stimulation of respiration was partially inhibited (by 50-70 and 50-60%, respectively). From these results it can be concluded that the increased rate of respiration after addition of fatty acids is coupled to ATP synthesis. A large part (50-60%) of this ATP is utilized by the (Na+ + K+)-ATPase",

author = "Plomp, {P. J.} and {van Roermund}, {C. W.} and Groen, {A. K.} and Meijer, {A. J.} and Tager, {J. M.}",

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T1 - MECHANISM OF THE STIMULATION OF RESPIRATION BY FATTY-ACIDS IN RAT-LIVER

AU - Plomp, P. J.

AU - van Roermund, C. W.

AU - Groen, A. K.

AU - Meijer, A. J.

AU - Tager, J. M.

PY - 1985

Y1 - 1985

N2 - The mechanism of stimulation of hepatic respiration by fatty acids was studied in isolated rat hepatocytes. Stimulation of respiration by fatty acids varied from about 35% to about 105% depending on chain length. The stimulatory effect of octanoate (1 mM) or oleate (0.5 mM) was prevented by oligomycin (2 micrograms/ml). With carboxyatractyloside (100 microM) and ouabain (2 mM) the stimulation of respiration was partially inhibited (by 50-70 and 50-60%, respectively). From these results it can be concluded that the increased rate of respiration after addition of fatty acids is coupled to ATP synthesis. A large part (50-60%) of this ATP is utilized by the (Na+ + K+)-ATPase

AB - The mechanism of stimulation of hepatic respiration by fatty acids was studied in isolated rat hepatocytes. Stimulation of respiration by fatty acids varied from about 35% to about 105% depending on chain length. The stimulatory effect of octanoate (1 mM) or oleate (0.5 mM) was prevented by oligomycin (2 micrograms/ml). With carboxyatractyloside (100 microM) and ouabain (2 mM) the stimulation of respiration was partially inhibited (by 50-70 and 50-60%, respectively). From these results it can be concluded that the increased rate of respiration after addition of fatty acids is coupled to ATP synthesis. A large part (50-60%) of this ATP is utilized by the (Na+ + K+)-ATPase

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DO - https://doi.org/10.1016/0014-5793(85)80161-7

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