TY - JOUR
T1 - Meijer and Vloedman’s histochemical demonstration of mitochondrial coupling obeys Lambert–Beer’s law in the myocardium
AU - Peters, Eva L.
AU - Comerford, David
AU - Vaz, Frédéric M.
AU - van der Laarse, Willem J.
PY - 2019/1/29
Y1 - 2019/1/29
N2 - Uncoupling of mitochondrial proton pumping and adenosine triphosphate (ATP) production lowers mitochondrial efficiency. Current methods to determine mitochondrial efficiency require substantial amounts of tissue and permeabilization or isolation procedures. A simple histochemical method has been described by Meijer and Vloedman (Histochemistry 69:217–232, 1980, https://doi.org/10.1007/BF00489769), but this was not quantitative. We found linear correlations between (1) absorbance and sections thickness and (2) absorbance and incubation time. Because the method obeys Lambert–Beer’s law, we can estimate ATP/O2 ratios for healthy and overloaded right-sided rat myocardium. We related mitochondrial efficiency to the ratio between cardiolipin and its precursor phosphatidylglycerol. We found a non-linear relationship between mitochondrial efficiency and this ratio, indicating that lower mitochondrial efficiency as found in experimental pulmonary hypertension may be due to altered composition of the mitochondrial inner membrane. We conclude that the histochemical method of Meijer and Vloedman can be applied to quantify mitochondrial efficiency.
AB - Uncoupling of mitochondrial proton pumping and adenosine triphosphate (ATP) production lowers mitochondrial efficiency. Current methods to determine mitochondrial efficiency require substantial amounts of tissue and permeabilization or isolation procedures. A simple histochemical method has been described by Meijer and Vloedman (Histochemistry 69:217–232, 1980, https://doi.org/10.1007/BF00489769), but this was not quantitative. We found linear correlations between (1) absorbance and sections thickness and (2) absorbance and incubation time. Because the method obeys Lambert–Beer’s law, we can estimate ATP/O2 ratios for healthy and overloaded right-sided rat myocardium. We related mitochondrial efficiency to the ratio between cardiolipin and its precursor phosphatidylglycerol. We found a non-linear relationship between mitochondrial efficiency and this ratio, indicating that lower mitochondrial efficiency as found in experimental pulmonary hypertension may be due to altered composition of the mitochondrial inner membrane. We conclude that the histochemical method of Meijer and Vloedman can be applied to quantify mitochondrial efficiency.
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85053229062&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/30159782
U2 - https://doi.org/10.1007/s00418-018-1716-3
DO - https://doi.org/10.1007/s00418-018-1716-3
M3 - Article
C2 - 30159782
SN - 0948-6143
VL - 151
SP - 85
EP - 90
JO - Histochemistry and Cell Biology
JF - Histochemistry and Cell Biology
IS - 1
ER -