TY - JOUR
T1 - Method comparison for N-glycan profiling: Towards the standardization of glycoanalytical technologies for cell line analysis
AU - Kotsias, Maximilianos
AU - Blanas, Athanasios
AU - van Vliet, Sandra J.
AU - Pirro, Martina
AU - Spencer, Daniel I. R.
AU - Kozak, Radoslaw P.
PY - 2019/1/1
Y1 - 2019/1/1
N2 - The study of protein N-glycosylation is essential in biological and biopharmaceutical research as N-glycans have been reported to regulate a wide range of physiological and pathological processes. Monitoring glycosylation in diagnosis, prognosis, as well as biopharmaceutical development and quality control are important research areas. A number of techniques for the analysis of protein N-glycosylation are currently available. Here we examine three methodologies routinely used for the release of N-glycans, in the effort to establish and standardize glycoproteomics technologies for quantitative glycan analysis from cultured cell lines. N-glycans from human gamma immunoglobulins (IgG), plasma and a pool of four cancer cell lines were released following three approaches and the performance of each method was evaluated.
AB - The study of protein N-glycosylation is essential in biological and biopharmaceutical research as N-glycans have been reported to regulate a wide range of physiological and pathological processes. Monitoring glycosylation in diagnosis, prognosis, as well as biopharmaceutical development and quality control are important research areas. A number of techniques for the analysis of protein N-glycosylation are currently available. Here we examine three methodologies routinely used for the release of N-glycans, in the effort to establish and standardize glycoproteomics technologies for quantitative glycan analysis from cultured cell lines. N-glycans from human gamma immunoglobulins (IgG), plasma and a pool of four cancer cell lines were released following three approaches and the performance of each method was evaluated.
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85072962885&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/31589631
U2 - https://doi.org/10.1371/journal.pone.0223270
DO - https://doi.org/10.1371/journal.pone.0223270
M3 - Article
C2 - 31589631
SN - 1932-6203
VL - 14
JO - PLOS ONE
JF - PLOS ONE
IS - 10
M1 - e0223270
ER -