Method comparison for N-glycan profiling: Towards the standardization of glycoanalytical technologies for cell line analysis

Maximilianos Kotsias, Athanasios Blanas, Sandra J. van Vliet, Martina Pirro, Daniel I. R. Spencer, Radoslaw P. Kozak

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The study of protein N-glycosylation is essential in biological and biopharmaceutical research as N-glycans have been reported to regulate a wide range of physiological and pathological processes. Monitoring glycosylation in diagnosis, prognosis, as well as biopharmaceutical development and quality control are important research areas. A number of techniques for the analysis of protein N-glycosylation are currently available. Here we examine three methodologies routinely used for the release of N-glycans, in the effort to establish and standardize glycoproteomics technologies for quantitative glycan analysis from cultured cell lines. N-glycans from human gamma immunoglobulins (IgG), plasma and a pool of four cancer cell lines were released following three approaches and the performance of each method was evaluated.
Original languageEnglish
Article numbere0223270
Issue number10
Publication statusPublished - 1 Jan 2019

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