TY - JOUR
T1 - Methodological aspects of in vivo axial loading in rodents
T2 - a systematic review
AU - Nepal, Ashwini Kumar
AU - van Essen, Hubertus W.
AU - de Jongh, Renate T.
AU - van Schoor, Natasja M.
AU - Otten, René H. J.
AU - Vanderschueren, Dirk
AU - Lips, Paul
AU - Bravenboer, Nathalie
N1 - Funding Information: This study was funded by the European Commission through MOVE-AGE, an Erasmus Mundus Joint Doctorate program (2011–2015). Publisher Copyright: © 2023, International Society of Musculoskeletal and Neuronal Interactions. All rights reserved.
PY - 2023/6/1
Y1 - 2023/6/1
N2 - Axial loading in rodents provides a controlled setting for mechanical loading, because load and subsequent strain, frequency, number of cycles and rest insertion between cycles, are precisely defined. These methodological aspects as well as factors, such as ovariectomy, aging, and disuse may affect the outcome of the loading test, including bone mass, structure, and bone mineral density. This review aims to overview methodological aspects and modifying factors in axial loading on bone outcomes. A systematic literature search was performed in bibliographic databases until December 2021, which resulted in 2183 articles. A total of 144 articles were selected for this review: 23 rat studies, 74 mouse studies, and 47 knock out (KO) mouse studies. Results indicated that peak load, frequency, and number of loading cycles mainly affected the outcomes of bone mass, structure, and density in both rat and mouse studies. It is crucial to consider methodological parameters and modifying factors such as age, sex-steroid deficiency, and disuse in loading protocols for the prediction of loading-related bone outcomes.
AB - Axial loading in rodents provides a controlled setting for mechanical loading, because load and subsequent strain, frequency, number of cycles and rest insertion between cycles, are precisely defined. These methodological aspects as well as factors, such as ovariectomy, aging, and disuse may affect the outcome of the loading test, including bone mass, structure, and bone mineral density. This review aims to overview methodological aspects and modifying factors in axial loading on bone outcomes. A systematic literature search was performed in bibliographic databases until December 2021, which resulted in 2183 articles. A total of 144 articles were selected for this review: 23 rat studies, 74 mouse studies, and 47 knock out (KO) mouse studies. Results indicated that peak load, frequency, and number of loading cycles mainly affected the outcomes of bone mass, structure, and density in both rat and mouse studies. It is crucial to consider methodological parameters and modifying factors such as age, sex-steroid deficiency, and disuse in loading protocols for the prediction of loading-related bone outcomes.
KW - Axial Loading
KW - Bone
KW - Mechanical Stress
KW - Mice
KW - Rats
UR - http://www.scopus.com/inward/record.url?scp=85161286302&partnerID=8YFLogxK
M3 - Review article
SN - 1108-7161
VL - 23
SP - 236
EP - 262
JO - Journal of Musculoskeletal and Neuronal Interactions
JF - Journal of Musculoskeletal and Neuronal Interactions
IS - 2
ER -