TY - JOUR
T1 - Methods for the identification and characterization of extracellular vesicles in cardiovascular studies
T2 - from exosomes to microvesicles
AU - Davidson, Sean M.
AU - Boulanger, Chantal M.
AU - Aikawa, Elena
AU - Badimon, Lina
AU - Barile, Lucio
AU - Binder, Christoph J.
AU - Brisson, Alain
AU - Buzas, Edit
AU - Emanueli, Costanza
AU - Jansen, Felix
AU - Katsur, Miroslava
AU - Lacroix, Romaric
AU - Lim, Sai Kiang
AU - Mackman, Nigel
AU - Mayr, Manuel
AU - Menasché, Philippe
AU - Nieuwland, Rienk
AU - Sahoo, Susmita
AU - Takov, Kaloyan
AU - Thum, Thomas
AU - Vader, Pieter
AU - Wauben, Marca H. M.
AU - Witwer, Kenneth
AU - Sluijter, Joost P. G.
N1 - Publisher Copyright: © The Author(s) 2022. Published by Oxford University Press on behalf of the European Society of Cardiology. All rights reserved.
PY - 2023/1/1
Y1 - 2023/1/1
N2 - Extracellular vesicles (EVs) are nanosized vesicles with a lipid bilayer that are released from cells of the cardiovascular system, and are considered important mediators of intercellular and extracellular communications. Two types of EVs of particular interest are exosomes and microvesicles, which have been identified in all tissue and body fluids and carry a variety of molecules including RNAs, proteins, and lipids. EVs have potential for use in the diagnosis and prognosis of cardiovascular diseases and as new therapeutic agents, particularly in the setting of myocardial infarction and heart failure. Despite their promise, technical challenges related to their small size make it challenging to accurately identify and characterize them, and to study EV-mediated processes. Here, we aim to provide the reader with an overview of the techniques and technologies available for the separation and characterization of EVs from different sources. Methods for determining the protein, RNA, and lipid content of EVs are discussed. The aim of this document is to provide guidance on critical methodological issues and highlight key points for consideration for the investigation of EVs in cardiovascular studies.
AB - Extracellular vesicles (EVs) are nanosized vesicles with a lipid bilayer that are released from cells of the cardiovascular system, and are considered important mediators of intercellular and extracellular communications. Two types of EVs of particular interest are exosomes and microvesicles, which have been identified in all tissue and body fluids and carry a variety of molecules including RNAs, proteins, and lipids. EVs have potential for use in the diagnosis and prognosis of cardiovascular diseases and as new therapeutic agents, particularly in the setting of myocardial infarction and heart failure. Despite their promise, technical challenges related to their small size make it challenging to accurately identify and characterize them, and to study EV-mediated processes. Here, we aim to provide the reader with an overview of the techniques and technologies available for the separation and characterization of EVs from different sources. Methods for determining the protein, RNA, and lipid content of EVs are discussed. The aim of this document is to provide guidance on critical methodological issues and highlight key points for consideration for the investigation of EVs in cardiovascular studies.
KW - Biodistribution
KW - Blood
KW - Cardiovascular diseases
KW - Exosomes
KW - Extracellular vesicle composition
KW - Heart
KW - Microvesicles
KW - Therapeutics
UR - http://www.scopus.com/inward/record.url?scp=85159573041&partnerID=8YFLogxK
U2 - https://doi.org/10.1093/cvr/cvac031
DO - https://doi.org/10.1093/cvr/cvac031
M3 - Review article
C2 - 35325061
SN - 0008-6363
VL - 119
SP - 45
EP - 63
JO - Cardiovascular research
JF - Cardiovascular research
IS - 1
ER -