Methylation-specific PCR unraveled

Sarah Derks; Marjolein H.F.M. Lentjes; Debby M.E.I. Hellebrekers; Adriaan P. De Bruïne; James G. Herman; Manon Van Engeland

Methylation-specific PCR unraveled

Sarah Derks, Marjolein H.F.M. Lentjes, Debby M.E.I. Hellebrekers, Adriaan P. De Bruïne, James G. Herman, Manon Van Engeland

Research output: Contribution to journal › Review article › Academic › peer-review

118 Citations (Scopus)

Abstract

Methylation-specific PCR (MSP) is a simple, quick and cost-effective method to analyze the DNA methylation status of virtually any group of CpG sites within a CpG island. The technique comprises two parts: (1) sodium bisulfite conversion of unmethylated cytosine's to uracil under conditions whereby methylated cytosines remains unchanged and (2) detection of the bisulfite induced sequence differences by PCR using specific primer sets for both unmethylated and methylated DNA. This review discusses the critical parameters of MSP and presents an overview of the available MSP variants and the (clinical) applications.

Original language	English
Pages (from-to)	291-299
Number of pages	9
Journal	Cellular oncology
Volume	26
Issue number	5-6
Publication status	Published - 2004

Keywords

DNA methylation
Epigenetics
Methylation-specific PCR
Tumor marker
Tumor suppressor gene silencing

Cite this

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title = "Methylation-specific PCR unraveled",

abstract = "Methylation-specific PCR (MSP) is a simple, quick and cost-effective method to analyze the DNA methylation status of virtually any group of CpG sites within a CpG island. The technique comprises two parts: (1) sodium bisulfite conversion of unmethylated cytosine's to uracil under conditions whereby methylated cytosines remains unchanged and (2) detection of the bisulfite induced sequence differences by PCR using specific primer sets for both unmethylated and methylated DNA. This review discusses the critical parameters of MSP and presents an overview of the available MSP variants and the (clinical) applications.",

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author = "Sarah Derks and Lentjes, {Marjolein H.F.M.} and Hellebrekers, {Debby M.E.I.} and {De Bru{\"i}ne}, {Adriaan P.} and Herman, {James G.} and {Van Engeland}, Manon",

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language = "English",

volume = "26",

pages = "291--299",

journal = "Cellular oncology",

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T1 - Methylation-specific PCR unraveled

AU - Derks, Sarah

AU - Lentjes, Marjolein H.F.M.

AU - Hellebrekers, Debby M.E.I.

AU - De Bruïne, Adriaan P.

AU - Herman, James G.

AU - Van Engeland, Manon

PY - 2004

Y1 - 2004

N2 - Methylation-specific PCR (MSP) is a simple, quick and cost-effective method to analyze the DNA methylation status of virtually any group of CpG sites within a CpG island. The technique comprises two parts: (1) sodium bisulfite conversion of unmethylated cytosine's to uracil under conditions whereby methylated cytosines remains unchanged and (2) detection of the bisulfite induced sequence differences by PCR using specific primer sets for both unmethylated and methylated DNA. This review discusses the critical parameters of MSP and presents an overview of the available MSP variants and the (clinical) applications.

AB - Methylation-specific PCR (MSP) is a simple, quick and cost-effective method to analyze the DNA methylation status of virtually any group of CpG sites within a CpG island. The technique comprises two parts: (1) sodium bisulfite conversion of unmethylated cytosine's to uracil under conditions whereby methylated cytosines remains unchanged and (2) detection of the bisulfite induced sequence differences by PCR using specific primer sets for both unmethylated and methylated DNA. This review discusses the critical parameters of MSP and presents an overview of the available MSP variants and the (clinical) applications.

KW - DNA methylation

KW - Epigenetics

KW - Methylation-specific PCR

KW - Tumor marker

KW - Tumor suppressor gene silencing

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M3 - Review article

C2 - 15623939

SN - 1570-5870

VL - 26

SP - 291

EP - 299

JO - Cellular oncology

JF - Cellular oncology

IS - 5-6

ER -

Methylation-specific PCR unraveled

Abstract

Keywords

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