TY - JOUR
T1 - MIBlood-EV
T2 - Minimal information to enhance the quality and reproducibility of blood extracellular vesicle research
AU - Lucien, Fabrice
AU - Gustafson, Dakota
AU - Lenassi, Metka
AU - Li, Bo
AU - Teske, Jacob J.
AU - Boilard, Eric
AU - von Hohenberg, Katharina Clemm
AU - Falcón-Perez, Juan Manual
AU - Gualerzi, Alice
AU - Reale, Antonia
AU - Jones, Jennifer C.
AU - Lässer, Cecilia
AU - Lawson, Charlotte
AU - Nazarenko, Irina
AU - O'Driscoll, Lorraine
AU - Pink, Ryan
AU - Siljander, Pia R. M.
AU - Soekmadji, Carolina
AU - Hendrix, An
AU - Welsh, Joshua A.
AU - Witwer, Kenneth W.
AU - Nieuwland, Rienk
N1 - Funding Information: The implementation of MIBlood‐EV will be driven by the commitment of EV research groups to promote reproducibility by ensuring transparent reporting of blood sample quality and derivatives used in EV studies. To achieve widespread adoption, we recommend the endorsement of the MIBlood‐EV by other international societies with long‐standing collaboration with ISEV, including ISAC and ISTH. Furthermore, we encourage support from the International Society for Biological and Environmental Repositories, the global biobanking organization in charge of disseminating best practices for biorepositories, and the European Liquid Biopsy Society. By promoting the adoption of the MIBlood‐EV, we can improve experimental rigor and reproducibility in EV research, accelerating the translation of discoveries into clinical practice. Publisher Copyright: © 2023 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals LLC on behalf of International Society for Extracellular Vesicles.
PY - 2023/12/1
Y1 - 2023/12/1
N2 - Blood is the most commonly used body fluid for extracellular vesicle (EV) research. The composition of a blood sample and its derivatives (i.e., plasma and serum) are not only donor-dependent but also influenced by collection and preparation protocols. Since there are hundreds of pre-analytical protocols and over forty variables, the development of standard operating procedures for EV research is very challenging. To improve the reproducibility of blood EV research, the International Society for Extracellular Vesicles (ISEV) Blood EV Task Force proposes standardized reporting of (i) the applied blood collection and preparation protocol and (ii) the quality of the prepared plasma and serum samples. Gathering detailed information will provide insight into the performance of the protocols and more effectively identify potential confounders in the prepared plasma and serum samples. To collect this information, the ISEV Blood EV Task Force created the Minimal Information for Blood EV research (MIBlood-EV), a tool to record and report information about pre-analytical protocols used for plasma and serum preparation as well as assays used to assess the quality of these preparations. This tool does not require modifications of established local pre-analytical protocols and can be easily implemented to enhance existing databases thereby enabling evidence-based optimization of pre-analytical protocols through meta-analysis. Taken together, insight into the quality of prepared plasma and serum samples will (i) improve the quality of biobanks for EV research, (ii) guide the exchange of plasma and serum samples between biobanks and laboratories, (iii) facilitate inter-laboratory comparative EV studies, and (iv) improve the peer review process.
AB - Blood is the most commonly used body fluid for extracellular vesicle (EV) research. The composition of a blood sample and its derivatives (i.e., plasma and serum) are not only donor-dependent but also influenced by collection and preparation protocols. Since there are hundreds of pre-analytical protocols and over forty variables, the development of standard operating procedures for EV research is very challenging. To improve the reproducibility of blood EV research, the International Society for Extracellular Vesicles (ISEV) Blood EV Task Force proposes standardized reporting of (i) the applied blood collection and preparation protocol and (ii) the quality of the prepared plasma and serum samples. Gathering detailed information will provide insight into the performance of the protocols and more effectively identify potential confounders in the prepared plasma and serum samples. To collect this information, the ISEV Blood EV Task Force created the Minimal Information for Blood EV research (MIBlood-EV), a tool to record and report information about pre-analytical protocols used for plasma and serum preparation as well as assays used to assess the quality of these preparations. This tool does not require modifications of established local pre-analytical protocols and can be easily implemented to enhance existing databases thereby enabling evidence-based optimization of pre-analytical protocols through meta-analysis. Taken together, insight into the quality of prepared plasma and serum samples will (i) improve the quality of biobanks for EV research, (ii) guide the exchange of plasma and serum samples between biobanks and laboratories, (iii) facilitate inter-laboratory comparative EV studies, and (iv) improve the peer review process.
KW - biomarker
KW - blood
KW - extracellular vesicles
KW - liquid biopsy
KW - quality control
KW - reproducibility
KW - standardization
UR - http://www.scopus.com/inward/record.url?scp=85178921111&partnerID=8YFLogxK
U2 - https://doi.org/10.1002/jev2.12385
DO - https://doi.org/10.1002/jev2.12385
M3 - Article
C2 - 38063210
SN - 2001-3078
VL - 12
JO - Journal of extracellular vesicles
JF - Journal of extracellular vesicles
IS - 12
M1 - 12385
ER -