Mice without phosphatidylcholine transfer protein have no defects in the secretion of phosphatidylcholine into bile or into lung airspaces

A. van Helvoort; A. de Brouwer; R. Ottenhoff; J. F. Brouwers; J. Wijnholds; J. H. Beijnen; A. Rijneveld; T. van der Poll; M. A. van der Valk; D. Majoor; W. Voorhout; K. W. Wirtz; R. P. Elferink; P. Borst

doi:https://doi.org/10.1073/pnas.96.20.11501

Mice without phosphatidylcholine transfer protein have no defects in the secretion of phosphatidylcholine into bile or into lung airspaces

A. van Helvoort, A. de Brouwer, R. Ottenhoff, J. F. Brouwers, J. Wijnholds, J. H. Beijnen, A. Rijneveld, T. van der Poll, M. A. van der Valk, D. Majoor, W. Voorhout, K. W. Wirtz, R. P. Elferink, P. Borst

Other Departments

Research output: Contribution to journal › Article › Academic › peer-review

65 Citations (Scopus)

Abstract

Phosphatidylcholine transfer protein (Pc-tp) is a highly specific carrier of phosphatidylcholine (PC) without known function. Proposed functions include the supply of PC required for secretion into bile or lung air space (surfactant) and the facilitation of enzymatic reactions involving PC synthesis or breakdown. To test these functions, we generated knock-out mice unable to make Pc-tp. Remarkably, these mice are normal and have no defect in any of the postulated Pc-tp functions analyzed. The lipid content and composition of the bile, as well as lung surfactant secretion and composition, of Pc-tp (-/-) mice, is normal. The lack of a Pc-tp contribution to biliary lipid secretion is in agreement with our finding that Pc-tp is down-regulated in adult mouse liver: whereas Pc-tp is abundant in the liver of mouse pups, Pc-tp levels decrease > 10-fold around 2 wk after birth, when bile formation starts. In adult mice, Pc-tp levels are high only in epididymis, testis, kidney, and bone marrow-derived mast cells. Absence of Pc-tp in bone marrow-derived mast cells does not affect their lipid composition or PC synthesis and degradation. We discuss how PC might reach the canalicular membrane of the hepatocyte for secretion into the bile, if not by Pc-tp

Original language	English
Pages (from-to)	11501-11506
Journal	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume	96
Issue number	20
DOIs	https://doi.org/10.1073/pnas.96.20.11501
Publication status	Published - 1999

Access to Document

https://doi.org/10.1073/pnas.96.20.11501

Cite this

van Helvoort, A., de Brouwer, A., Ottenhoff, R., Brouwers, J. F., Wijnholds, J., Beijnen, J. H., Rijneveld, A., van der Poll, T., van der Valk, M. A., Majoor, D., Voorhout, W., Wirtz, K. W., Elferink, R. P., & Borst, P. (1999). Mice without phosphatidylcholine transfer protein have no defects in the secretion of phosphatidylcholine into bile or into lung airspaces. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 96(20), 11501-11506. https://doi.org/10.1073/pnas.96.20.11501

@article{d280e92aa4254122b4901c5b27b0cc53,

title = "Mice without phosphatidylcholine transfer protein have no defects in the secretion of phosphatidylcholine into bile or into lung airspaces",

abstract = "Phosphatidylcholine transfer protein (Pc-tp) is a highly specific carrier of phosphatidylcholine (PC) without known function. Proposed functions include the supply of PC required for secretion into bile or lung air space (surfactant) and the facilitation of enzymatic reactions involving PC synthesis or breakdown. To test these functions, we generated knock-out mice unable to make Pc-tp. Remarkably, these mice are normal and have no defect in any of the postulated Pc-tp functions analyzed. The lipid content and composition of the bile, as well as lung surfactant secretion and composition, of Pc-tp (-/-) mice, is normal. The lack of a Pc-tp contribution to biliary lipid secretion is in agreement with our finding that Pc-tp is down-regulated in adult mouse liver: whereas Pc-tp is abundant in the liver of mouse pups, Pc-tp levels decrease > 10-fold around 2 wk after birth, when bile formation starts. In adult mice, Pc-tp levels are high only in epididymis, testis, kidney, and bone marrow-derived mast cells. Absence of Pc-tp in bone marrow-derived mast cells does not affect their lipid composition or PC synthesis and degradation. We discuss how PC might reach the canalicular membrane of the hepatocyte for secretion into the bile, if not by Pc-tp",

author = "{van Helvoort}, A. and {de Brouwer}, A. and R. Ottenhoff and Brouwers, {J. F.} and J. Wijnholds and Beijnen, {J. H.} and A. Rijneveld and {van der Poll}, T. and {van der Valk}, {M. A.} and D. Majoor and W. Voorhout and Wirtz, {K. W.} and Elferink, {R. P.} and P. Borst",

year = "1999",

doi = "https://doi.org/10.1073/pnas.96.20.11501",

language = "English",

volume = "96",

pages = "11501--11506",

journal = "PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA",

issn = "0027-8424",

publisher = "National Acad Sciences",

number = "20",

}

van Helvoort, A, de Brouwer, A, Ottenhoff, R, Brouwers, JF, Wijnholds, J, Beijnen, JH, Rijneveld, A, van der Poll, T, van der Valk, MA, Majoor, D, Voorhout, W, Wirtz, KW, Elferink, RP & Borst, P 1999, 'Mice without phosphatidylcholine transfer protein have no defects in the secretion of phosphatidylcholine into bile or into lung airspaces', PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 96, no. 20, pp. 11501-11506. https://doi.org/10.1073/pnas.96.20.11501

Mice without phosphatidylcholine transfer protein have no defects in the secretion of phosphatidylcholine into bile or into lung airspaces. / van Helvoort, A.; de Brouwer, A.; Ottenhoff, R. et al.
In: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol. 96, No. 20, 1999, p. 11501-11506.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Mice without phosphatidylcholine transfer protein have no defects in the secretion of phosphatidylcholine into bile or into lung airspaces

AU - van Helvoort, A.

AU - de Brouwer, A.

AU - Ottenhoff, R.

AU - Brouwers, J. F.

AU - Wijnholds, J.

AU - Beijnen, J. H.

AU - Rijneveld, A.

AU - van der Poll, T.

AU - van der Valk, M. A.

AU - Majoor, D.

AU - Voorhout, W.

AU - Wirtz, K. W.

AU - Elferink, R. P.

AU - Borst, P.

PY - 1999

Y1 - 1999

N2 - Phosphatidylcholine transfer protein (Pc-tp) is a highly specific carrier of phosphatidylcholine (PC) without known function. Proposed functions include the supply of PC required for secretion into bile or lung air space (surfactant) and the facilitation of enzymatic reactions involving PC synthesis or breakdown. To test these functions, we generated knock-out mice unable to make Pc-tp. Remarkably, these mice are normal and have no defect in any of the postulated Pc-tp functions analyzed. The lipid content and composition of the bile, as well as lung surfactant secretion and composition, of Pc-tp (-/-) mice, is normal. The lack of a Pc-tp contribution to biliary lipid secretion is in agreement with our finding that Pc-tp is down-regulated in adult mouse liver: whereas Pc-tp is abundant in the liver of mouse pups, Pc-tp levels decrease > 10-fold around 2 wk after birth, when bile formation starts. In adult mice, Pc-tp levels are high only in epididymis, testis, kidney, and bone marrow-derived mast cells. Absence of Pc-tp in bone marrow-derived mast cells does not affect their lipid composition or PC synthesis and degradation. We discuss how PC might reach the canalicular membrane of the hepatocyte for secretion into the bile, if not by Pc-tp

AB - Phosphatidylcholine transfer protein (Pc-tp) is a highly specific carrier of phosphatidylcholine (PC) without known function. Proposed functions include the supply of PC required for secretion into bile or lung air space (surfactant) and the facilitation of enzymatic reactions involving PC synthesis or breakdown. To test these functions, we generated knock-out mice unable to make Pc-tp. Remarkably, these mice are normal and have no defect in any of the postulated Pc-tp functions analyzed. The lipid content and composition of the bile, as well as lung surfactant secretion and composition, of Pc-tp (-/-) mice, is normal. The lack of a Pc-tp contribution to biliary lipid secretion is in agreement with our finding that Pc-tp is down-regulated in adult mouse liver: whereas Pc-tp is abundant in the liver of mouse pups, Pc-tp levels decrease > 10-fold around 2 wk after birth, when bile formation starts. In adult mice, Pc-tp levels are high only in epididymis, testis, kidney, and bone marrow-derived mast cells. Absence of Pc-tp in bone marrow-derived mast cells does not affect their lipid composition or PC synthesis and degradation. We discuss how PC might reach the canalicular membrane of the hepatocyte for secretion into the bile, if not by Pc-tp

U2 - https://doi.org/10.1073/pnas.96.20.11501

DO - https://doi.org/10.1073/pnas.96.20.11501

M3 - Article

C2 - 10500206

SN - 0027-8424

VL - 96

SP - 11501

EP - 11506

JO - PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

JF - PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

IS - 20

ER -

van Helvoort A, de Brouwer A, Ottenhoff R, Brouwers JF, Wijnholds J, Beijnen JH et al. Mice without phosphatidylcholine transfer protein have no defects in the secretion of phosphatidylcholine into bile or into lung airspaces. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 1999;96(20):11501-11506. doi: https://doi.org/10.1073/pnas.96.20.11501