TY - JOUR
T1 - Micellar lipid composition profoundly affects LXR-dependent cholesterol transport across CaCo2 cells
AU - Petruzzelli, Michele
AU - Groen, Albert K.
AU - van Erpecum, Karel J.
AU - Vrins, Carlos
AU - van der Velde, Astrid E.
AU - Portincasa, Piero
AU - Palasciano, Giuseppe
AU - van Berge Henegouwen, Gerard P.
AU - Sasso, Giuseppe Lo
AU - Morgano, Annalisa
AU - Moschetta, Antonio
PY - 2009
Y1 - 2009
N2 - Intraluminal phospholipids affect micellar solubilization and absorption of cholesterol. We here study cholesterol transport from taurocholate-phospholipid-cholesterol micelles to CaCo2 cells, and associated effects on ABC-A1 mediated cholesterol efflux. Micellar incorporation of egg-yolk-phosphatidylcholine markedly increased apical retention of the sterol with decreased expression of ABC-A1, an effect that is prevented by synthetic liver X receptor (LXR) or retinoid X receptor (RXR) agonists. On the other hand, incorporation of lyso-phosphatidylcholine (LysoPC) increased ABC-A1-HDL-dependent basolateral cholesterol efflux, an effect that is abated when LXR is silenced. Thus, the modulation of cholesterol metabolism via intraluminal phospholipids is related to the activity of the oxysterol nuclear receptor LXR. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved
AB - Intraluminal phospholipids affect micellar solubilization and absorption of cholesterol. We here study cholesterol transport from taurocholate-phospholipid-cholesterol micelles to CaCo2 cells, and associated effects on ABC-A1 mediated cholesterol efflux. Micellar incorporation of egg-yolk-phosphatidylcholine markedly increased apical retention of the sterol with decreased expression of ABC-A1, an effect that is prevented by synthetic liver X receptor (LXR) or retinoid X receptor (RXR) agonists. On the other hand, incorporation of lyso-phosphatidylcholine (LysoPC) increased ABC-A1-HDL-dependent basolateral cholesterol efflux, an effect that is abated when LXR is silenced. Thus, the modulation of cholesterol metabolism via intraluminal phospholipids is related to the activity of the oxysterol nuclear receptor LXR. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved
U2 - https://doi.org/10.1016/j.febslet.2009.03.021
DO - https://doi.org/10.1016/j.febslet.2009.03.021
M3 - Article
C2 - 19303409
SN - 0014-5793
VL - 583
SP - 1274
EP - 1280
JO - FEBS letters
JF - FEBS letters
IS - 8
ER -