TY - JOUR
T1 - Microvasculature Features Derived from Hybrid EPI MRI in Non-Enhancing Adult-Type Diffuse Glioma Subtypes
AU - Arzanforoosh, Fatemeh
AU - van der Voort, Sebastian R.
AU - Incekara, Fatih
AU - Vincent, Arnaud
AU - van den Bent, Martin
AU - Kros, Johan M.
AU - Smits, Marion
AU - Warnert, Esther A. H.
N1 - Funding Information: This research was funded by the Dutch Cancer Society (KWF): “Non-invasive phenotyping of molecular brain tumor profiles using novel advanced MR imaging and analysis”, EMCR 2015–7859. E.A.H.W. was funded by a “Veni Vernieuwingsimpuls” from the Dutch Research Council entitled “Food for thought: Oxygen delivery to the brain”, grant number 91619121. S.R.v.d.V. was funded by the Dutch Research Council open technology program, grant number 17079. Publisher Copyright: © 2023 by the authors.
PY - 2023/4/1
Y1 - 2023/4/1
N2 - In this study, we used the vessel size imaging (VSI) MRI technique to characterize the microvasculature features of three subtypes of adult-type diffuse glioma lacking enhancement. Thirty-eight patients with confirmed non-enhancing glioma were categorized into three subtypes: Oligo (IDH-mut&1p/19q-codeleted), Astro (IDH-mut), and GBM (IDH-wt). The VSI technique provided quantitative maps of cerebral blood volume (CBV), microvasculature (µCBV), and vessel size for each patient. Additionally, tissue samples of 21 patients were histopathologically analyzed, and microvasculature features were quantified. Both MRI- and histology-derived features were compared across the three glioma subtypes with ANOVA or Kruskal–Wallis tests. Group averages of CBV, μCBV, and vessel size were significantly different between the three glioma subtypes (p < 0.01). Astro (IDH-mut) had a significantly lower CBV and µCBV compared to Oligo (IDH-mut&1p/19q-codeleted) (p = 0.004 and p = 0.001, respectively), and a higher average vessel size compared to GBM (IDH-wt) (p = 0.01). The histopathological analysis showed that GBM (IDH-wt) possessed vessels with more irregular shapes than the two other subtypes (p < 0.05). VSI provides a good insight into the microvasculature characteristics of the three adult-type glioma subtypes even when lacking enhancement. Further investigations into the specificity of VSI to differentiate glioma subtypes are thus warranted.
AB - In this study, we used the vessel size imaging (VSI) MRI technique to characterize the microvasculature features of three subtypes of adult-type diffuse glioma lacking enhancement. Thirty-eight patients with confirmed non-enhancing glioma were categorized into three subtypes: Oligo (IDH-mut&1p/19q-codeleted), Astro (IDH-mut), and GBM (IDH-wt). The VSI technique provided quantitative maps of cerebral blood volume (CBV), microvasculature (µCBV), and vessel size for each patient. Additionally, tissue samples of 21 patients were histopathologically analyzed, and microvasculature features were quantified. Both MRI- and histology-derived features were compared across the three glioma subtypes with ANOVA or Kruskal–Wallis tests. Group averages of CBV, μCBV, and vessel size were significantly different between the three glioma subtypes (p < 0.01). Astro (IDH-mut) had a significantly lower CBV and µCBV compared to Oligo (IDH-mut&1p/19q-codeleted) (p = 0.004 and p = 0.001, respectively), and a higher average vessel size compared to GBM (IDH-wt) (p = 0.01). The histopathological analysis showed that GBM (IDH-wt) possessed vessels with more irregular shapes than the two other subtypes (p < 0.05). VSI provides a good insight into the microvasculature characteristics of the three adult-type glioma subtypes even when lacking enhancement. Further investigations into the specificity of VSI to differentiate glioma subtypes are thus warranted.
KW - cerebral blood volume
KW - glioma
KW - microvessel
KW - molecular typing
KW - perfusion imaging
KW - vessel size imaging
UR - http://www.scopus.com/inward/record.url?scp=85152557833&partnerID=8YFLogxK
U2 - https://doi.org/10.3390/cancers15072135
DO - https://doi.org/10.3390/cancers15072135
M3 - Article
C2 - 37046796
SN - 2072-6694
VL - 15
JO - Cancers
JF - Cancers
IS - 7
M1 - 2135
ER -