TY - JOUR
T1 - Mimicking the cardiac cycle in intact cardiomyocytes using diastolic and systolic force clamps; measuring power output
AU - Helmes, Michiel
AU - Najafi, Aref
AU - Palmer, Bradley M.
AU - Breel, Ernst
AU - Rijnveld, Niek
AU - Iannuzzi, Davide
AU - van der Velden, Jolanda
PY - 2016/7/1
Y1 - 2016/7/1
N2 - Aims: A single isolated cardiomyocyte is the smallest functional unit of the heart. Yet, all single isolated cardiomyocyte experiments have been limited by the lack of proper methods that could reproduce a physiological cardiac cycle. We aimed to investigate the contractile properties of a single cardiomyocyte that correctly mimic the cardiac cycle. Methods and results: By adjusting the parameters of the feedback loop, using a suitably engineered feedback system and recording the developed force and the length of a single rat cardiomyocyte during contraction and relaxation, we were able to construct force-length (FL) relations analogous to the pressure-volume (PV) relations at the whole heart level. From the cardiac loop graphs, we obtained, for the first time, the power generated by one single cardiomyocyte. Conclusion: Here, we introduce a new approach that by combining mechanics, electronics, and a new type optical force transducer can measure the FL relationship of a single isolated cardiomyocyte undergoing a mechanical loop that mimics the PV cycle of a beating heart.
AB - Aims: A single isolated cardiomyocyte is the smallest functional unit of the heart. Yet, all single isolated cardiomyocyte experiments have been limited by the lack of proper methods that could reproduce a physiological cardiac cycle. We aimed to investigate the contractile properties of a single cardiomyocyte that correctly mimic the cardiac cycle. Methods and results: By adjusting the parameters of the feedback loop, using a suitably engineered feedback system and recording the developed force and the length of a single rat cardiomyocyte during contraction and relaxation, we were able to construct force-length (FL) relations analogous to the pressure-volume (PV) relations at the whole heart level. From the cardiac loop graphs, we obtained, for the first time, the power generated by one single cardiomyocyte. Conclusion: Here, we introduce a new approach that by combining mechanics, electronics, and a new type optical force transducer can measure the FL relationship of a single isolated cardiomyocyte undergoing a mechanical loop that mimics the PV cycle of a beating heart.
KW - Cardiomyocyte function
KW - Force-length relation
KW - Microtechnology
U2 - https://doi.org/10.1093/cvr/cvw072
DO - https://doi.org/10.1093/cvr/cvw072
M3 - Article
C2 - 27037258
SN - 0008-6363
VL - 111
SP - 66
EP - 73
JO - Cardiovascular research
JF - Cardiovascular research
IS - 1
ER -