TY - JOUR
T1 - Molecular characterization of addiction systems of plasmids encoding extended-spectrum β-lactamases in Escherichia coli
AU - Mnif, Basma
AU - Vimont, Sophie
AU - Boyd, Anders
AU - Bourit, Emilie
AU - Picard, Bertrand
AU - Branger, Catherine
AU - Denamur, Erick
AU - Arlet, Guillaume
PY - 2010/5/27
Y1 - 2010/5/27
N2 - Objectives: Escherichia coli producing CTX-M-type extended-spectrum β-lactamases (ESBLs) are spreading worldwide. The aim of this work was to investigate the addiction systems carried by the replicons involved in the emergence and spread of ESBLs in relation to ESBL and replicon types. Methods: A collection of 125 TEM, SHV and CTX-M ESBL-producing E. coli isolates and their 125 transconjugants or transformants was analysed. Five plasmid protein antitoxin-regulated systems and three plasmid antisense RNA-regulated systems were sought by PCR. Results: Two hundred and ninety-eight plasmid addiction systems were detected in the parental strains (mean 2.38, range 0-6 per strain) and 86 were detected in the recipient strains (mean 0.69, range 0-5 per strain). PemKI, CcdAB, Hok-Sok and VagCD were the most frequently represented systems in both recipient and parental strains. The parental SHV and CTX-M ESBL-producing strains had more addiction systems than the TEM ESBL producers. In the recipient strains, the frequency of addiction systems was significantly higher in IncF plasmids. Among the IncF replicons carrying CTX-M-type enzymes, the frequency of addiction systems was significantly higher in IncF plasmids carrying CTX-M-15 (mean 3.5) or CTX-M-9 (mean 4) than in those carrying CTX-M-14 (mean 0.6). Conclusions: In E. coli producing CTX-M-15 or CTX-M-9 ESBLs, plasmids bearing the blaCTX-M gene have multiple addiction systems that could contribute to their maintenance in host strains. © The Author 2010.
AB - Objectives: Escherichia coli producing CTX-M-type extended-spectrum β-lactamases (ESBLs) are spreading worldwide. The aim of this work was to investigate the addiction systems carried by the replicons involved in the emergence and spread of ESBLs in relation to ESBL and replicon types. Methods: A collection of 125 TEM, SHV and CTX-M ESBL-producing E. coli isolates and their 125 transconjugants or transformants was analysed. Five plasmid protein antitoxin-regulated systems and three plasmid antisense RNA-regulated systems were sought by PCR. Results: Two hundred and ninety-eight plasmid addiction systems were detected in the parental strains (mean 2.38, range 0-6 per strain) and 86 were detected in the recipient strains (mean 0.69, range 0-5 per strain). PemKI, CcdAB, Hok-Sok and VagCD were the most frequently represented systems in both recipient and parental strains. The parental SHV and CTX-M ESBL-producing strains had more addiction systems than the TEM ESBL producers. In the recipient strains, the frequency of addiction systems was significantly higher in IncF plasmids. Among the IncF replicons carrying CTX-M-type enzymes, the frequency of addiction systems was significantly higher in IncF plasmids carrying CTX-M-15 (mean 3.5) or CTX-M-9 (mean 4) than in those carrying CTX-M-14 (mean 0.6). Conclusions: In E. coli producing CTX-M-15 or CTX-M-9 ESBLs, plasmids bearing the blaCTX-M gene have multiple addiction systems that could contribute to their maintenance in host strains. © The Author 2010.
KW - Conjugation, Genetic
KW - DNA, Bacterial/genetics
KW - Escherichia coli Proteins/genetics
KW - Escherichia coli/genetics
KW - Genes, Bacterial
KW - Plasmids
KW - Polymerase Chain Reaction
KW - Transformation, Genetic
KW - beta-Lactamases/genetics
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=77954698242&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/20507859
U2 - https://doi.org/10.1093/jac/dkq181
DO - https://doi.org/10.1093/jac/dkq181
M3 - Article
C2 - 20507859
SN - 0305-7453
VL - 65
SP - 1599
EP - 1603
JO - The Journal of antimicrobial chemotherapy
JF - The Journal of antimicrobial chemotherapy
IS - 8
ER -