Molecular comparison of calcineurin inhibitor-induced fibrogenic responses in protocol renal transplant biopsies

Marian C. Roos-van Groningen; Eduard M. Scholten; Patrick M. Lelieveld; Ajda T. Rowshani; Hans J. Baelde; Ingeborg M. Bajema; Sandrine Florquin; Frederike J. Bemelman; Emile de Heer; Johan W. de Fijter; Jan A. Bruijn; Michael Eikmans

doi:https://doi.org/10.1681/ASN.2005080891

Molecular comparison of calcineurin inhibitor-induced fibrogenic responses in protocol renal transplant biopsies

Marian C. Roos-van Groningen, Eduard M. Scholten, Patrick M. Lelieveld, Ajda T. Rowshani, Hans J. Baelde, Ingeborg M. Bajema, Sandrine Florquin, Frederike J. Bemelman, Emile de Heer, Johan W. de Fijter, Jan A. Bruijn, Michael Eikmans

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

The calcineurin inhibitor cyclosporine (CsA) induces a fibrogenic response that may lead to scarring of the renal allograft. This study investigated whether tacrolimus, a novel calcineurin inhibitor, exerts fibrogenic effects to a similar extent. Sixty patients were enrolled in a randomized study: 29 received CsA, and 31 received tacrolimus. Patients were subjected to tailored exposure-controlled calcineurin inhibitor regimens. Protocol biopsies were obtained at the time of transplantation and 6 and 12 mo after transplantation. Cortical TGF-beta and collagens alpha1(I) and alpha1(III) mRNA steady-state levels were determined with real-time PCR. The extent of protein deposition of TGF-beta, alpha-smooth muscle actin, and interstitial collagens in the renal cortex was quantified with computer-assisted image analysis. The extent of interstitial collagen deposition measured with Sirius red and the accumulation of alpha-smooth muscle actin and TGF-beta protein after 6 and 12 mo were similar for both immunosuppressive regimens. mRNA levels of TGF-beta and collagens alpha1(I) and alpha1(III) were not significantly different in the treatment groups either. It is concluded that the fibrogenic response in renal allografts is similar in patients who receive CsA-based regimens and patients who receive tacrolimus-based regimens

Original language	English
Pages (from-to)	881-888
Journal	Journal of the American Society of Nephrology
Volume	17
Issue number	3
DOIs	https://doi.org/10.1681/ASN.2005080891
Publication status	Published - 2006

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https://doi.org/10.1681/ASN.2005080891

Cite this

Roos-van Groningen, M. C., Scholten, E. M., Lelieveld, P. M., Rowshani, A. T., Baelde, H. J., Bajema, I. M., Florquin, S., Bemelman, F. J., de Heer, E., de Fijter, J. W., Bruijn, J. A., & Eikmans, M. (2006). Molecular comparison of calcineurin inhibitor-induced fibrogenic responses in protocol renal transplant biopsies. Journal of the American Society of Nephrology, 17(3), 881-888. https://doi.org/10.1681/ASN.2005080891

@article{874055b6394041179c5282d6dfaf0ceb,

title = "Molecular comparison of calcineurin inhibitor-induced fibrogenic responses in protocol renal transplant biopsies",

abstract = "The calcineurin inhibitor cyclosporine (CsA) induces a fibrogenic response that may lead to scarring of the renal allograft. This study investigated whether tacrolimus, a novel calcineurin inhibitor, exerts fibrogenic effects to a similar extent. Sixty patients were enrolled in a randomized study: 29 received CsA, and 31 received tacrolimus. Patients were subjected to tailored exposure-controlled calcineurin inhibitor regimens. Protocol biopsies were obtained at the time of transplantation and 6 and 12 mo after transplantation. Cortical TGF-beta and collagens alpha1(I) and alpha1(III) mRNA steady-state levels were determined with real-time PCR. The extent of protein deposition of TGF-beta, alpha-smooth muscle actin, and interstitial collagens in the renal cortex was quantified with computer-assisted image analysis. The extent of interstitial collagen deposition measured with Sirius red and the accumulation of alpha-smooth muscle actin and TGF-beta protein after 6 and 12 mo were similar for both immunosuppressive regimens. mRNA levels of TGF-beta and collagens alpha1(I) and alpha1(III) were not significantly different in the treatment groups either. It is concluded that the fibrogenic response in renal allografts is similar in patients who receive CsA-based regimens and patients who receive tacrolimus-based regimens",

author = "{Roos-van Groningen}, {Marian C.} and Scholten, {Eduard M.} and Lelieveld, {Patrick M.} and Rowshani, {Ajda T.} and Baelde, {Hans J.} and Bajema, {Ingeborg M.} and Sandrine Florquin and Bemelman, {Frederike J.} and {de Heer}, Emile and {de Fijter}, {Johan W.} and Bruijn, {Jan A.} and Michael Eikmans",

year = "2006",

doi = "https://doi.org/10.1681/ASN.2005080891",

language = "English",

volume = "17",

pages = "881--888",

journal = "Journal of the American Society of Nephrology",

issn = "1046-6673",

publisher = "American Society of Nephrology",

number = "3",

}

Roos-van Groningen, MC, Scholten, EM, Lelieveld, PM, Rowshani, AT, Baelde, HJ, Bajema, IM, Florquin, S , Bemelman, FJ, de Heer, E, de Fijter, JW, Bruijn, JA & Eikmans, M 2006, 'Molecular comparison of calcineurin inhibitor-induced fibrogenic responses in protocol renal transplant biopsies', Journal of the American Society of Nephrology, vol. 17, no. 3, pp. 881-888. https://doi.org/10.1681/ASN.2005080891

TY - JOUR

T1 - Molecular comparison of calcineurin inhibitor-induced fibrogenic responses in protocol renal transplant biopsies

AU - Roos-van Groningen, Marian C.

AU - Scholten, Eduard M.

AU - Lelieveld, Patrick M.

AU - Rowshani, Ajda T.

AU - Baelde, Hans J.

AU - Bajema, Ingeborg M.

AU - Florquin, Sandrine

AU - Bemelman, Frederike J.

AU - de Heer, Emile

AU - de Fijter, Johan W.

AU - Bruijn, Jan A.

AU - Eikmans, Michael

PY - 2006

Y1 - 2006

N2 - The calcineurin inhibitor cyclosporine (CsA) induces a fibrogenic response that may lead to scarring of the renal allograft. This study investigated whether tacrolimus, a novel calcineurin inhibitor, exerts fibrogenic effects to a similar extent. Sixty patients were enrolled in a randomized study: 29 received CsA, and 31 received tacrolimus. Patients were subjected to tailored exposure-controlled calcineurin inhibitor regimens. Protocol biopsies were obtained at the time of transplantation and 6 and 12 mo after transplantation. Cortical TGF-beta and collagens alpha1(I) and alpha1(III) mRNA steady-state levels were determined with real-time PCR. The extent of protein deposition of TGF-beta, alpha-smooth muscle actin, and interstitial collagens in the renal cortex was quantified with computer-assisted image analysis. The extent of interstitial collagen deposition measured with Sirius red and the accumulation of alpha-smooth muscle actin and TGF-beta protein after 6 and 12 mo were similar for both immunosuppressive regimens. mRNA levels of TGF-beta and collagens alpha1(I) and alpha1(III) were not significantly different in the treatment groups either. It is concluded that the fibrogenic response in renal allografts is similar in patients who receive CsA-based regimens and patients who receive tacrolimus-based regimens

AB - The calcineurin inhibitor cyclosporine (CsA) induces a fibrogenic response that may lead to scarring of the renal allograft. This study investigated whether tacrolimus, a novel calcineurin inhibitor, exerts fibrogenic effects to a similar extent. Sixty patients were enrolled in a randomized study: 29 received CsA, and 31 received tacrolimus. Patients were subjected to tailored exposure-controlled calcineurin inhibitor regimens. Protocol biopsies were obtained at the time of transplantation and 6 and 12 mo after transplantation. Cortical TGF-beta and collagens alpha1(I) and alpha1(III) mRNA steady-state levels were determined with real-time PCR. The extent of protein deposition of TGF-beta, alpha-smooth muscle actin, and interstitial collagens in the renal cortex was quantified with computer-assisted image analysis. The extent of interstitial collagen deposition measured with Sirius red and the accumulation of alpha-smooth muscle actin and TGF-beta protein after 6 and 12 mo were similar for both immunosuppressive regimens. mRNA levels of TGF-beta and collagens alpha1(I) and alpha1(III) were not significantly different in the treatment groups either. It is concluded that the fibrogenic response in renal allografts is similar in patients who receive CsA-based regimens and patients who receive tacrolimus-based regimens

U2 - https://doi.org/10.1681/ASN.2005080891

DO - https://doi.org/10.1681/ASN.2005080891

M3 - Article

C2 - 16467444

SN - 1046-6673

VL - 17

SP - 881

EP - 888

JO - Journal of the American Society of Nephrology

JF - Journal of the American Society of Nephrology

IS - 3

ER -