TY - JOUR
T1 - Monitoring blood volume and saturation using superficial fibre optic reflectance spectroscopy during PDT of actinic keratosis
AU - Middelburg, Tom A.
AU - Kanick, Stephen C.
AU - de Haas, Ellen R. M.
AU - Sterenborg, Henricus J. C. M.
AU - Amelink, Arjen
AU - Neumann, Martino H. A. M.
AU - Robinson, Dominic J.
PY - 2011
Y1 - 2011
N2 - Optically monitoring the vascular physiology during photodynamic therapy (PDT) may help understand patient-specific treatment outcome. However, diffuse optical techniques have failed to observe changes herein, probably by optically sampling too deep. Therefore, we investigated using differential path-length spectroscopy (DPS) to obtain superficial measurements of vascular physiology in actinic keratosis (AK) skin. The AK-specific DPS interrogation depth was chosen up to 400 microns in depth, based on the thickness of AK histology samples. During light fractionated aminolevulinic acid-PDT, reflectance spectra were analyzed to yield quantitative estimates of blood volume and saturation. Blood volume showed significant lesion-specific changes during PDT without a general trend for all lesions and saturation remained high during PDT. This study shows that DPS allows optically monitoring the superficial blood volume and saturation during skin PDT. The patient-specific variability supports the need for dosimetric measurements. In DPS, the lesion-specific optimal interrogation depth can be varied based on lesion thickness. [GRAPHICS] Experimental setup of differential path-length spectroscopy
AB - Optically monitoring the vascular physiology during photodynamic therapy (PDT) may help understand patient-specific treatment outcome. However, diffuse optical techniques have failed to observe changes herein, probably by optically sampling too deep. Therefore, we investigated using differential path-length spectroscopy (DPS) to obtain superficial measurements of vascular physiology in actinic keratosis (AK) skin. The AK-specific DPS interrogation depth was chosen up to 400 microns in depth, based on the thickness of AK histology samples. During light fractionated aminolevulinic acid-PDT, reflectance spectra were analyzed to yield quantitative estimates of blood volume and saturation. Blood volume showed significant lesion-specific changes during PDT without a general trend for all lesions and saturation remained high during PDT. This study shows that DPS allows optically monitoring the superficial blood volume and saturation during skin PDT. The patient-specific variability supports the need for dosimetric measurements. In DPS, the lesion-specific optimal interrogation depth can be varied based on lesion thickness. [GRAPHICS] Experimental setup of differential path-length spectroscopy
U2 - https://doi.org/10.1002/jbio.201100053
DO - https://doi.org/10.1002/jbio.201100053
M3 - Article
C2 - 21842485
SN - 1864-063X
VL - 4
SP - 721
EP - 730
JO - Journal of Biophotonics
JF - Journal of Biophotonics
IS - 10
ER -