Monoclonal antibody JSB-1 detects a highly conserved epitope on the P-glycoprotein associated with multi-drug-resistance

R. J. Scheper; J. W. Bulte; J. G. Brakkee; J. J. Quak; E. van der Schoot; A. J. Balm; C. J. Meijer; H. J. Broxterman; C. M. Kuiper; J. Lankelma; W.J.M. Bulte; A.J.M. Palm; H.M. Pinedo

doi:https://doi.org/10.1002/ijc.2910420314

Monoclonal antibody JSB-1 detects a highly conserved epitope on the P-glycoprotein associated with multi-drug-resistance

R. J. Scheper, J. W. Bulte, J. G. Brakkee, J. J. Quak, E. van der Schoot, A. J. Balm, C. J. Meijer, H. J. Broxterman, C. M. Kuiper, J. Lankelma, W.J.M. Bulte, A.J.M. Palm, H.M. Pinedo

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Abstract

Resistance to multiple chemotherapeutic agents is a common clinical problem in the treatment of cancer. This resistance may occur before primary therapy or be acquired during treatment. We have generated a monoclonal antibody (MAb) (JSB-I), specific for a conserved epitope on the plasma membrane 170- to 180-kDa glycoprotein, the expression of which is strongly correlated with the degree of multi-drug resistance (MDR). JSB-I strongly binds to both Chinese-hamster-derived MDR cell lines and human MDR cell lines, including cell lines derived from lung and ovary. A drug-sensitive revertant line, and the corresponding drug-sensitive parent lines, showed only weak reactivity or none at all. JSB-I reacts strongly to air-dried or acetone-fixed cells and therefore has potential value for diagnostic detection of MDR cells in human tumor samples

Original language	English
Pages (from-to)	389-394
Journal	International journal of cancer. Journal international du cancer
Volume	42
Issue number	3
DOIs	https://doi.org/10.1002/ijc.2910420314
Publication status	Published - 1988

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https://research.vumc.nl/ws/files/9070603/8323

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Scheper, R. J., Bulte, J. W., Brakkee, J. G., Quak, J. J., van der Schoot, E., Balm, A. J., Meijer, C. J., Broxterman, H. J., Kuiper, C. M., Lankelma, J., Bulte, W. J. M., Palm, A. J. M., & Pinedo, H. M. (1988). Monoclonal antibody JSB-1 detects a highly conserved epitope on the P-glycoprotein associated with multi-drug-resistance. International journal of cancer. Journal international du cancer, 42(3), 389-394. https://doi.org/10.1002/ijc.2910420314

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title = "Monoclonal antibody JSB-1 detects a highly conserved epitope on the P-glycoprotein associated with multi-drug-resistance",

abstract = "Resistance to multiple chemotherapeutic agents is a common clinical problem in the treatment of cancer. This resistance may occur before primary therapy or be acquired during treatment. We have generated a monoclonal antibody (MAb) (JSB-I), specific for a conserved epitope on the plasma membrane 170- to 180-kDa glycoprotein, the expression of which is strongly correlated with the degree of multi-drug resistance (MDR). JSB-I strongly binds to both Chinese-hamster-derived MDR cell lines and human MDR cell lines, including cell lines derived from lung and ovary. A drug-sensitive revertant line, and the corresponding drug-sensitive parent lines, showed only weak reactivity or none at all. JSB-I reacts strongly to air-dried or acetone-fixed cells and therefore has potential value for diagnostic detection of MDR cells in human tumor samples",

author = "Scheper, {R. J.} and Bulte, {J. W.} and Brakkee, {J. G.} and Quak, {J. J.} and {van der Schoot}, E. and Balm, {A. J.} and Meijer, {C. J.} and Broxterman, {H. J.} and Kuiper, {C. M.} and J. Lankelma and W.J.M. Bulte and A.J.M. Palm and H.M. Pinedo",

year = "1988",

doi = "https://doi.org/10.1002/ijc.2910420314",

language = "English",

volume = "42",

pages = "389--394",

journal = "International journal of cancer. Journal international du cancer",

issn = "0020-7136",

publisher = "Wiley-Liss Inc.",

number = "3",

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Scheper, RJ, Bulte, JW, Brakkee, JG, Quak, JJ, van der Schoot, E, Balm, AJ, Meijer, CJ, Broxterman, HJ, Kuiper, CM, Lankelma, J, Bulte, WJM, Palm, AJM & Pinedo, HM 1988, 'Monoclonal antibody JSB-1 detects a highly conserved epitope on the P-glycoprotein associated with multi-drug-resistance', International journal of cancer. Journal international du cancer, vol. 42, no. 3, pp. 389-394. https://doi.org/10.1002/ijc.2910420314

TY - JOUR

T1 - Monoclonal antibody JSB-1 detects a highly conserved epitope on the P-glycoprotein associated with multi-drug-resistance

AU - Scheper, R. J.

AU - Bulte, J. W.

AU - Brakkee, J. G.

AU - Quak, J. J.

AU - van der Schoot, E.

AU - Balm, A. J.

AU - Meijer, C. J.

AU - Broxterman, H. J.

AU - Kuiper, C. M.

AU - Lankelma, J.

AU - Bulte, W.J.M.

AU - Palm, A.J.M.

AU - Pinedo, H.M.

PY - 1988

Y1 - 1988

N2 - Resistance to multiple chemotherapeutic agents is a common clinical problem in the treatment of cancer. This resistance may occur before primary therapy or be acquired during treatment. We have generated a monoclonal antibody (MAb) (JSB-I), specific for a conserved epitope on the plasma membrane 170- to 180-kDa glycoprotein, the expression of which is strongly correlated with the degree of multi-drug resistance (MDR). JSB-I strongly binds to both Chinese-hamster-derived MDR cell lines and human MDR cell lines, including cell lines derived from lung and ovary. A drug-sensitive revertant line, and the corresponding drug-sensitive parent lines, showed only weak reactivity or none at all. JSB-I reacts strongly to air-dried or acetone-fixed cells and therefore has potential value for diagnostic detection of MDR cells in human tumor samples

AB - Resistance to multiple chemotherapeutic agents is a common clinical problem in the treatment of cancer. This resistance may occur before primary therapy or be acquired during treatment. We have generated a monoclonal antibody (MAb) (JSB-I), specific for a conserved epitope on the plasma membrane 170- to 180-kDa glycoprotein, the expression of which is strongly correlated with the degree of multi-drug resistance (MDR). JSB-I strongly binds to both Chinese-hamster-derived MDR cell lines and human MDR cell lines, including cell lines derived from lung and ovary. A drug-sensitive revertant line, and the corresponding drug-sensitive parent lines, showed only weak reactivity or none at all. JSB-I reacts strongly to air-dried or acetone-fixed cells and therefore has potential value for diagnostic detection of MDR cells in human tumor samples

U2 - https://doi.org/10.1002/ijc.2910420314

DO - https://doi.org/10.1002/ijc.2910420314

M3 - Article

C2 - 2458323

SN - 0020-7136

VL - 42

SP - 389

EP - 394

JO - International journal of cancer. Journal international du cancer

JF - International journal of cancer. Journal international du cancer

IS - 3

ER -