TY - JOUR
T1 - Monoclonal autoantibodies recognizing histone variants
AU - van Hemert, Formijn J.
AU - van Dam, Alje P.
AU - Jonk, Luigi J. C.
AU - Olivier, H. J.
AU - Destrée, J. T.
AU - Smeenk, Ruud J. T.
PY - 1988
Y1 - 1988
N2 - Balb/c mice were immunized with affinity purified Ro(SS-A) from human origin in order to allow the preparation of monoclonal anti-Ro(SS-A) antibodies. After fusion of mouse myeloma cells (line Sp2/0 A914) with spleen cells from one of these mice, anti-Ro(SS-A) monoclonals were not obtained, but, instead, two IgM producing hybridomas reactive with histone H1 and one with histone H2B. The specificity of the anti-H1 monoclonals was investigated by means of immunoblotting of very lysine-rich histone variants from mouse which were separated by two-dimensional gelelectrophoresis. One of them (CLB-ANA 105) has H1 o specificity with respect to the histone variants of mouse and man, but recognizes H5 as well as H5 from Xenopus laevis Another monoclonal (CLB-ANA 108) reacts with the variant H1 c from mouse, exclusively. From the way these monoclonals were produced, we postulate that they were not the result of immunization, but comprise specifities of naturally occurring autoantibodies. © 1988 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
AB - Balb/c mice were immunized with affinity purified Ro(SS-A) from human origin in order to allow the preparation of monoclonal anti-Ro(SS-A) antibodies. After fusion of mouse myeloma cells (line Sp2/0 A914) with spleen cells from one of these mice, anti-Ro(SS-A) monoclonals were not obtained, but, instead, two IgM producing hybridomas reactive with histone H1 and one with histone H2B. The specificity of the anti-H1 monoclonals was investigated by means of immunoblotting of very lysine-rich histone variants from mouse which were separated by two-dimensional gelelectrophoresis. One of them (CLB-ANA 105) has H1 o specificity with respect to the histone variants of mouse and man, but recognizes H5 as well as H5 from Xenopus laevis Another monoclonal (CLB-ANA 108) reacts with the variant H1 c from mouse, exclusively. From the way these monoclonals were produced, we postulate that they were not the result of immunization, but comprise specifities of naturally occurring autoantibodies. © 1988 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0023769892&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/3410513
U2 - https://doi.org/10.3109/08820138809052960
DO - https://doi.org/10.3109/08820138809052960
M3 - Article
C2 - 3410513
SN - 0882-0139
VL - 17
SP - 195
EP - 215
JO - Immunological Investigations
JF - Immunological Investigations
IS - 3
ER -