TY - JOUR
T1 - mRNA Electroporation of Dendritic Cells with WT1, Survivin, and TriMix (a Mixture of caTLR4, CD40L, and CD70)
AU - Coosemans, An
AU - Tuyaerts, Sandra
AU - Morias, Kim
AU - Corthals, Jurgen
AU - Heirman, Carlo
AU - Thielemans, Kris
AU - van Gool, Stefaan W.
AU - Vergote, Ignace
AU - Amant, Frédéric
PY - 2016
Y1 - 2016
N2 - The immune system is a crucial player in the development of cancer. Once it is in imbalance and immunosuppressive mechanisms supporting tumor growth take over control, dendritic cell immunotherapy might offer a solution to restore the balance. There are several methods to manufacture dendritic cells but none of them has yet proven to be superior to others. In this chapter, we discuss the methodology using electroporation of mRNA encoding Wilms' tumor gene 1, survivin, and TriMix (mixture of caTLR4, CD40L, and CD70) to simultaneously load and mature dendritic cells
AB - The immune system is a crucial player in the development of cancer. Once it is in imbalance and immunosuppressive mechanisms supporting tumor growth take over control, dendritic cell immunotherapy might offer a solution to restore the balance. There are several methods to manufacture dendritic cells but none of them has yet proven to be superior to others. In this chapter, we discuss the methodology using electroporation of mRNA encoding Wilms' tumor gene 1, survivin, and TriMix (mixture of caTLR4, CD40L, and CD70) to simultaneously load and mature dendritic cells
U2 - https://doi.org/10.1007/978-1-4939-3625-0_18
DO - https://doi.org/10.1007/978-1-4939-3625-0_18
M3 - Article
C2 - 27236806
SN - 1064-3745
VL - 1428
SP - 277
EP - 283
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -