TY - JOUR
T1 - Mu- and delta-opioid receptors inhibitorily linked to dopamine-sensitive adenylate cyclase in rat striatum display a selectivity profile toward endogenous opioid peptides different from that of presynaptic mu, delta and kappa receptors
AU - Schoffelmeer, A. N M
AU - De Vries, T. J.
AU - Hogenboom, F.
AU - Mulder, A. H.
PY - 1993/1/1
Y1 - 1993/1/1
N2 - The apparent affinities of endogenous opioid peptides for non- competitively interacting mu and delta receptors, inhibitorily linked to dopamine (DA) D-1 receptor-stimulated adenylate cyclase, were investigated in superfused rat striatal slices exposed to 40 μM DA in the presence of 10 μM of the selective D-2 receptor antagonist (-)sulpiride. In the presence of peptidase inhibitors, a comparison was made with the apparent affinities of opioid peptides toward independent presynaptic opioid receptors in brain slices. β-Endorphin1-31 had an about 100-fold higher apparent affinity (EC50: 1 nM) toward presynaptic mu-opioid receptors, mediating inhibition of the electrically evoked neocortical [3H]norepinephrine release, than for the striatal adenylate cyclase-coupled mu·receptors. In contrast, the kappa- opioid receptor agonist dynorphin A1-13 displayed a similar apparent affinity (EC50: 0.1 μM) toward these functionally different mu receptors. Both Leu- and Met-enkephalin showed only a 3-fold higher apparent affinity (EC50: 30 nM) for presynaptic delta-opioid receptors, mediating inhibition of striatal [14C]acetylcholine release, than for presynaptic mu receptors. However, whereas Leu-enkephalin had a similar apparent affinity for presynaptic and adenylate cyclase-coupled delta receptors, Met-enkephalin displayed a 30-fold selectivity toward the latter receptors. Studying the inhibitory effect of Met-enkephalin on striatal adenylate cyclase stimulated by endogenously released (amphetamine-induced) DA, its very high affinity appeared to be inversely related to the activation of inhibitory DA D-2 receptors. These data reveal a unique agonist affinity profile of interacting adenylate cyclase-coupled mu and delta receptors, mediating postsynaptic inhibition of dopaminergic neurotransmission in rat striatum, with the naturally occurring opioid peptide Met-enkephalin as a highly selective agonist. The permissive role of DA D-2 receptors is discussed.
AB - The apparent affinities of endogenous opioid peptides for non- competitively interacting mu and delta receptors, inhibitorily linked to dopamine (DA) D-1 receptor-stimulated adenylate cyclase, were investigated in superfused rat striatal slices exposed to 40 μM DA in the presence of 10 μM of the selective D-2 receptor antagonist (-)sulpiride. In the presence of peptidase inhibitors, a comparison was made with the apparent affinities of opioid peptides toward independent presynaptic opioid receptors in brain slices. β-Endorphin1-31 had an about 100-fold higher apparent affinity (EC50: 1 nM) toward presynaptic mu-opioid receptors, mediating inhibition of the electrically evoked neocortical [3H]norepinephrine release, than for the striatal adenylate cyclase-coupled mu·receptors. In contrast, the kappa- opioid receptor agonist dynorphin A1-13 displayed a similar apparent affinity (EC50: 0.1 μM) toward these functionally different mu receptors. Both Leu- and Met-enkephalin showed only a 3-fold higher apparent affinity (EC50: 30 nM) for presynaptic delta-opioid receptors, mediating inhibition of striatal [14C]acetylcholine release, than for presynaptic mu receptors. However, whereas Leu-enkephalin had a similar apparent affinity for presynaptic and adenylate cyclase-coupled delta receptors, Met-enkephalin displayed a 30-fold selectivity toward the latter receptors. Studying the inhibitory effect of Met-enkephalin on striatal adenylate cyclase stimulated by endogenously released (amphetamine-induced) DA, its very high affinity appeared to be inversely related to the activation of inhibitory DA D-2 receptors. These data reveal a unique agonist affinity profile of interacting adenylate cyclase-coupled mu and delta receptors, mediating postsynaptic inhibition of dopaminergic neurotransmission in rat striatum, with the naturally occurring opioid peptide Met-enkephalin as a highly selective agonist. The permissive role of DA D-2 receptors is discussed.
UR - http://www.scopus.com/inward/record.url?scp=0027331474&partnerID=8YFLogxK
M3 - Article
C2 - 8229747
SN - 0022-3565
VL - 267
SP - 205
EP - 210
JO - Journal of Pharmacology and Experimental Therapeutics
JF - Journal of Pharmacology and Experimental Therapeutics
IS - 1
ER -