TY - JOUR
T1 - Mutational spectrum of ATRX aberrations in neuroblastoma and associated patient and tumor characteristics
AU - van Gerven, Michael R.
AU - Bozsaky, Eva
AU - Matser, Yvette A. H.
AU - Vosseberg, Julian
AU - Taschner-Mandl, Sabine
AU - Koster, Jan
AU - Tytgat, Godelieve A. M.
AU - Molenaar, Jan J.
AU - van den Boogaard, Marlinde
N1 - Funding Information: We would like to thank Matthias Fischer, Paul Thorner, and Susanne Fransson for sharing patient data. In addition, we want to thank Freek Manders for assistance with generation of the deletion figures. This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program under grant agreement numbers 716079 (Predict) and 826121 (iPC project). Publisher Copyright: © 2022 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.
PY - 2022/6
Y1 - 2022/6
N2 - Neuroblastoma is the most common extracranial solid tumor in children. The chromatin remodeler ATRX is frequently mutated in high-risk patients with a poor prognosis. Although many studies have reported ATRX aberrations and the associated clinical characteristics in neuroblastoma, a comprehensive overview is currently lacking. In this study, we extensively characterize the mutational spectrum of ATRX aberrations in neuroblastoma tumors reported in previous studies and present an overview of patient and tumor characteristics. We collected the data of a total of 127 neuroblastoma patients and three cell lines with ATRX aberrations originating from 20 papers. We subdivide the ATRX aberrations into nonsense, missense, and multiexon deletions (MEDs) and show that 68% of them are MEDs. Of these MEDs, 75% are predicted to be in-frame. Furthermore, we identify a missense mutational hotspot region in the helicase domain. We also confirm that all three ATRX mutation types are more often identified in patients diagnosed at an older age, but still approximately 40% of the patients are aged 5 years or younger at diagnosis. Surprisingly, we found that 11q deletions are enriched in neuroblastomas with ATRX deletions compared to a reference cohort, but not in neuroblastomas with ATRX point mutations. Taken together, our data emphasizes a distinct ATRX mutation spectrum in neuroblastoma, which should be considered when studying molecular phenotypes and therapeutic strategies.
AB - Neuroblastoma is the most common extracranial solid tumor in children. The chromatin remodeler ATRX is frequently mutated in high-risk patients with a poor prognosis. Although many studies have reported ATRX aberrations and the associated clinical characteristics in neuroblastoma, a comprehensive overview is currently lacking. In this study, we extensively characterize the mutational spectrum of ATRX aberrations in neuroblastoma tumors reported in previous studies and present an overview of patient and tumor characteristics. We collected the data of a total of 127 neuroblastoma patients and three cell lines with ATRX aberrations originating from 20 papers. We subdivide the ATRX aberrations into nonsense, missense, and multiexon deletions (MEDs) and show that 68% of them are MEDs. Of these MEDs, 75% are predicted to be in-frame. Furthermore, we identify a missense mutational hotspot region in the helicase domain. We also confirm that all three ATRX mutation types are more often identified in patients diagnosed at an older age, but still approximately 40% of the patients are aged 5 years or younger at diagnosis. Surprisingly, we found that 11q deletions are enriched in neuroblastomas with ATRX deletions compared to a reference cohort, but not in neuroblastomas with ATRX point mutations. Taken together, our data emphasizes a distinct ATRX mutation spectrum in neuroblastoma, which should be considered when studying molecular phenotypes and therapeutic strategies.
KW - 11q deletion
KW - ALT
KW - ATRX
KW - neuroblastoma
KW - pediatric oncology
UR - http://www.scopus.com/inward/record.url?scp=85128749909&partnerID=8YFLogxK
U2 - https://doi.org/10.1111/cas.15363
DO - https://doi.org/10.1111/cas.15363
M3 - Article
C2 - 35384159
SN - 1347-9032
VL - 113
SP - 2167
EP - 2178
JO - Cancer science
JF - Cancer science
IS - 6
ER -