TY - JOUR
T1 - N-glycomic signature of stage II colorectal cancer and its association with the tumor microenvironment
AU - Boyaval, Fanny
AU - van Zeijl, René
AU - Dalebout, Hans
AU - Holst, Stephanie
AU - van Pelt, Gabi
AU - Fariña-Sarasqueta, Arantza
AU - Mesker, Wilma
AU - Tollenaar, Rob
AU - Morreau, Hans
AU - Wuhrer, Manfred
AU - Heijs, Bram
N1 - Funding Information: Acknowledgments—We thank the staff of the Center of Proteomics and Metabolomics Facility for technical assistance and the department of pathology for providing the tissues. This research is supported by the “Leids Universiteits Fonds” (Netherlands), grant 6212, concerning the “Dr Anne Bosma Fonds”, a legacy of the late Dr Anne Bosma, a former pathologist at the Amsterdam University Medical Center and the Leiden University Medical Center. Publisher Copyright: © 2021 American Society for Biochemistry and Molecular Biology Inc.. All rights reserved.
PY - 2021
Y1 - 2021
N2 - The choice for adjuvant chemotherapy in stage II colorectal cancer is controversial as many patients are cured by surgery alone and it is difficult to identify patients with high risk of recurrence of the disease. There is a need for better stratification of this group of patients. Mass spectrometry imaging could identify patients at risk. We report here the N-glycosylation signatures of the different cell populations in a group of stage II colorectal cancer tissue samples. The cancer cells, compared with normal epithelial cells, have increased levels of sialylation and high-mannose glycans, as well as decreased levels of fucosylation and highly branched N-glycans. When looking at the interface between cancer and its microenvironment, it seems that the cancer N-glycosylation signature spreads into the surrounding stroma at the invasive front of the tumor. This finding was more outspoken in patients with a worse outcome within this sample group.
AB - The choice for adjuvant chemotherapy in stage II colorectal cancer is controversial as many patients are cured by surgery alone and it is difficult to identify patients with high risk of recurrence of the disease. There is a need for better stratification of this group of patients. Mass spectrometry imaging could identify patients at risk. We report here the N-glycosylation signatures of the different cell populations in a group of stage II colorectal cancer tissue samples. The cancer cells, compared with normal epithelial cells, have increased levels of sialylation and high-mannose glycans, as well as decreased levels of fucosylation and highly branched N-glycans. When looking at the interface between cancer and its microenvironment, it seems that the cancer N-glycosylation signature spreads into the surrounding stroma at the invasive front of the tumor. This finding was more outspoken in patients with a worse outcome within this sample group.
UR - http://www.scopus.com/inward/record.url?scp=85104283426&partnerID=8YFLogxK
U2 - https://doi.org/10.1074/MCP.RA120.002215
DO - https://doi.org/10.1074/MCP.RA120.002215
M3 - Article
C2 - 33082215
SN - 1535-9476
VL - 20
JO - Molecular and Cellular Proteomics
JF - Molecular and Cellular Proteomics
M1 - 100057
ER -