Naturally occurring antibodies isolated from PD patients inhibit synuclein seeding in vitro and recognize Lewy pathology

Xinyi Li, Wouter Koudstaal, Lauren Fletcher, Martha Costa, Margot van Winsen, Berdien Siregar, Hanna Inganäs, Julie Kim, Elissa Keogh, Jeremy Macedo, Trevin Holland, Stuart Perry, Frederique Bard, Jeroen J. Hoozemans, Jaap Goudsmit, Adrian Apetri, Gabriel Pascual

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29 Citations (Scopus)


Deposition of α-synuclein into Lewy bodies and Lewy neurites is the hallmark of Parkinson’s disease (PD). It is hypothesized that α-synuclein pathology spreads by a “prion-like” mechanism (i.e., by seeded aggregation or templated misfolding). Therefore, various extracellular α-synuclein conformers and/or posttranslational modifications may serve as biomarkers of disease or potential targets for novel interventions. To explore whether the antibody repertoires of PD patients contain anti-α-synuclein antibodies that can potentially be used as markers or immunotherapy, we interrogated peripheral IgG + memory B cells from PD patients for reactivity to α-synuclein. In total, ten somatically mutated antibodies were recovered, suggesting the presence of an ongoing antigen-driven immune response. The three antibodies that had the highest affinity to recombinant full-length α-synuclein, aSyn-323.1, aSyn-336.1 and aSyn-338.1, were characterized further and shown to recognize epitopes in the C terminus of α-synuclein with binding affinities between 0.3 and 2.8 μM. Furthermore, all three antibodies were able to neutralize the “seeding” of intracellular synuclein aggregates in an in vitro α-synuclein seeding assay. Finally, differential reactivities were observed for all three human anti-α-synuclein antibodies across tissue treatment conditions by immunohistochemistry. Our results suggest that the memory B-cell repertoire of PD patients might represent a potential source of biomarkers and therapies.
Original languageEnglish
Pages (from-to)825-836
Number of pages12
JournalActa Neuropathologica
Issue number5
Publication statusPublished - 1 May 2019


  • Alpha-synuclein protein
  • Lewy bodies
  • Lewy neurites
  • Memory B cell
  • Monoclonal antibody
  • Parkinson’s disease

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