TY - JOUR
T1 - New liposomal doxorubicin nanoformulation for osteosarcoma
T2 - Drug release kinetic study based on thermo and pH sensitivity
AU - Haghiralsadat, F.
AU - Amoabediny, G.
AU - Sheikhha, M.H.
AU - Zandieh-doulabi, B.
AU - Naderinezhad, S.
AU - Helder, M.N.
AU - Forouzanfar, T.
PY - 2017/9/1
Y1 - 2017/9/1
N2 - A novel approach was developed for the preparation of stealth controlled-release liposomal doxorubicin. Various liposomal formulations were prepared by employing both thin film and pH gradient hydration techniques. The optimum formulation contained phospholipid and cholesterol in 1:0.43 molar ratios in the presence of 3% DSPE-mPEG (2000). The liposomal formulation was evaluated by determining mean size of vesicle, encapsulation efficiency, polydispersity index, zeta potentials, carrier's functionalization, and surface morphology. The vesicle size, encapsulation efficiency, polydispersity index, and zeta potentials of purposed formula were 93.61 nm, 82.8%, 0.14, and −23, respectively. Vesicles were round-shaped and smooth-surfaced entities with sharp boundaries. In addition, two colorimetric methods for cytotoxicity assay were compared and the IC50 (the half maximal inhibitory concentration) of both methods for encapsulated doxorubicin was determined to be 0.1 μg/ml. The results of kinetic drug release were investigated at several different temperatures and pH levels, which showed that purposed formulation was thermo and pH sensitive.
AB - A novel approach was developed for the preparation of stealth controlled-release liposomal doxorubicin. Various liposomal formulations were prepared by employing both thin film and pH gradient hydration techniques. The optimum formulation contained phospholipid and cholesterol in 1:0.43 molar ratios in the presence of 3% DSPE-mPEG (2000). The liposomal formulation was evaluated by determining mean size of vesicle, encapsulation efficiency, polydispersity index, zeta potentials, carrier's functionalization, and surface morphology. The vesicle size, encapsulation efficiency, polydispersity index, and zeta potentials of purposed formula were 93.61 nm, 82.8%, 0.14, and −23, respectively. Vesicles were round-shaped and smooth-surfaced entities with sharp boundaries. In addition, two colorimetric methods for cytotoxicity assay were compared and the IC50 (the half maximal inhibitory concentration) of both methods for encapsulated doxorubicin was determined to be 0.1 μg/ml. The results of kinetic drug release were investigated at several different temperatures and pH levels, which showed that purposed formulation was thermo and pH sensitive.
KW - cytotoxicity
KW - drug delivery
KW - liposome characterization
KW - osteosarcoma
KW - release kinetics
UR - http://www.scopus.com/inward/record.url?scp=85017428270&partnerID=8YFLogxK
U2 - https://doi.org/10.1111/cbdd.12953
DO - https://doi.org/10.1111/cbdd.12953
M3 - Article
C2 - 28120466
SN - 1747-0277
VL - 90
SP - 368
EP - 379
JO - Chemical Biology and Drug Design
JF - Chemical Biology and Drug Design
IS - 3
ER -
