Noninvasive fetal genotyping of human platelet antigen-1a

P. G. Scheffer; A. Ait Soussan; O. J. H. M. Verhagen; G. C. M. L. Page-Christiaens; D. Oepkes; M. de Haas; C. E. van der Schoot

doi:https://doi.org/10.1111/j.1471-0528.2011.03039.x

Noninvasive fetal genotyping of human platelet antigen-1a

P. G. Scheffer, A. Ait Soussan, O. J. H. M. Verhagen, G. C. M. L. Page-Christiaens, D. Oepkes, M. de Haas, C. E. van der Schoot

Research output: Contribution to journal › Article › Academic › peer-review

59 Citations (Scopus)

Abstract

We describe a reliable noninvasive fetal human platelet antigen (HPA)-1a genotyping assay on a real-time polymerase chain reaction (PCR) platform using cell-free fetal DNA isolated from maternal blood. Nonspecific amplification of maternal cell-free DNA is overcome by pre-PCR digestion of the cell-free DNA with the Msp1 restriction enzyme. Noninvasive fetal HPA-1a genotyping offers a safe method for alloimmunised pregnant women to determine whether their fetus is at risk of fetal or neonatal alloimmune thrombocytopenia (FNAIT) and whether interventions to prevent intracranial haemorrhage are required. The availability of this test is relevant to the ongoing debate on screening pregnancies for HPA-1a-mediated FNAIT

Original language	English
Pages (from-to)	1392-1395
Journal	BJOG
Volume	118
Issue number	11
DOIs	https://doi.org/10.1111/j.1471-0528.2011.03039.x
Publication status	Published - 2011

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https://doi.org/10.1111/j.1471-0528.2011.03039.x

Cite this

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title = "Noninvasive fetal genotyping of human platelet antigen-1a",

abstract = "We describe a reliable noninvasive fetal human platelet antigen (HPA)-1a genotyping assay on a real-time polymerase chain reaction (PCR) platform using cell-free fetal DNA isolated from maternal blood. Nonspecific amplification of maternal cell-free DNA is overcome by pre-PCR digestion of the cell-free DNA with the Msp1 restriction enzyme. Noninvasive fetal HPA-1a genotyping offers a safe method for alloimmunised pregnant women to determine whether their fetus is at risk of fetal or neonatal alloimmune thrombocytopenia (FNAIT) and whether interventions to prevent intracranial haemorrhage are required. The availability of this test is relevant to the ongoing debate on screening pregnancies for HPA-1a-mediated FNAIT",

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AU - Scheffer, P. G.

AU - Ait Soussan, A.

AU - Verhagen, O. J. H. M.

AU - Page-Christiaens, G. C. M. L.

AU - Oepkes, D.

AU - de Haas, M.

AU - van der Schoot, C. E.

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AB - We describe a reliable noninvasive fetal human platelet antigen (HPA)-1a genotyping assay on a real-time polymerase chain reaction (PCR) platform using cell-free fetal DNA isolated from maternal blood. Nonspecific amplification of maternal cell-free DNA is overcome by pre-PCR digestion of the cell-free DNA with the Msp1 restriction enzyme. Noninvasive fetal HPA-1a genotyping offers a safe method for alloimmunised pregnant women to determine whether their fetus is at risk of fetal or neonatal alloimmune thrombocytopenia (FNAIT) and whether interventions to prevent intracranial haemorrhage are required. The availability of this test is relevant to the ongoing debate on screening pregnancies for HPA-1a-mediated FNAIT

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DO - https://doi.org/10.1111/j.1471-0528.2011.03039.x

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