Novel amphiphilic block-copolymer forming stable micelles and interpolyelectrolyte complexes with DNA for efficient gene delivery

Zeliha Guler Gokce; Semra Zuhal Birol; Nataliya Mitina; Khrystyna Harhay; Nataliya Finiuk; Valentina Glasunova; Rostyslav Stoika; Sebnem Ercelen; Alexander Zaichenko

doi:https://doi.org/10.1080/00914037.2020.1740988

Novel amphiphilic block-copolymer forming stable micelles and interpolyelectrolyte complexes with DNA for efficient gene delivery

Zeliha Guler Gokce, Semra Zuhal Birol, Nataliya Mitina, Khrystyna Harhay, Nataliya Finiuk, Valentina Glasunova, Rostyslav Stoika, Sebnem Ercelen, Alexander Zaichenko

Research output: Contribution to journal › Article › Academic › peer-review

3 Citations (Scopus)

Abstract

Novel amphiphilic poly(DMAEMA)-block-poly(NVP-co-BA-co-AEM) (BP83-1) forms stable micelles and BP83-1/pDNA complexes possessing controlled size, charge and enhanced aggregation degree. It was found that the formation of the micelles by BP83-1 is necessary for successful DNA binding and compaction. The polyamphiphile micelle aggregation degree defined their crucial effect on the compaction and morphology of polyplexes. Strong compaction of the DNA upon interaction with polymer at CMC value, positive charge, and high stability of the polyplex are key factors promoting the penetration of DNA through bio-surfaces that define the efficiency of gene delivery in mammalian cells.

Original language	English
Pages (from-to)	554-573
Number of pages	20
Journal	International Journal of Polymeric Materials and Polymeric Biomaterials
Volume	70
Issue number	8
Early online date	2020
DOIs	https://doi.org/10.1080/00914037.2020.1740988
Publication status	Published - 2021
Externally published	Yes

Keywords

DNA compaction
gene delivery
micelles
polyamphiphile
polyplex formation

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https://doi.org/10.1080/00914037.2020.1740988

Cite this

Guler Gokce, Z., Birol, S. Z., Mitina, N., Harhay, K., Finiuk, N., Glasunova, V., Stoika, R., Ercelen, S., & Zaichenko, A. (2021). Novel amphiphilic block-copolymer forming stable micelles and interpolyelectrolyte complexes with DNA for efficient gene delivery. International Journal of Polymeric Materials and Polymeric Biomaterials, 70(8), 554-573. https://doi.org/10.1080/00914037.2020.1740988

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title = "Novel amphiphilic block-copolymer forming stable micelles and interpolyelectrolyte complexes with DNA for efficient gene delivery",

abstract = "Novel amphiphilic poly(DMAEMA)-block-poly(NVP-co-BA-co-AEM) (BP83-1) forms stable micelles and BP83-1/pDNA complexes possessing controlled size, charge and enhanced aggregation degree. It was found that the formation of the micelles by BP83-1 is necessary for successful DNA binding and compaction. The polyamphiphile micelle aggregation degree defined their crucial effect on the compaction and morphology of polyplexes. Strong compaction of the DNA upon interaction with polymer at CMC value, positive charge, and high stability of the polyplex are key factors promoting the penetration of DNA through bio-surfaces that define the efficiency of gene delivery in mammalian cells.",

keywords = "DNA compaction, gene delivery, micelles, polyamphiphile, polyplex formation",

author = "{Guler Gokce}, Zeliha and Birol, {Semra Zuhal} and Nataliya Mitina and Khrystyna Harhay and Nataliya Finiuk and Valentina Glasunova and Rostyslav Stoika and Sebnem Ercelen and Alexander Zaichenko",

note = "Funding Information: The authors express their thanks to TUBITAK, Turkey for the financial support to project numbered 109S258 (SBAG-UKRAYNA-3) and the State Committee of Ukraine for Science and Innovation for funding of the project numbered M/417-2011. Publisher Copyright: {\textcopyright} 2020 Taylor & Francis Group, LLC.",

year = "2021",

doi = "https://doi.org/10.1080/00914037.2020.1740988",

language = "English",

volume = "70",

pages = "554--573",

journal = "International Journal of Polymeric Materials and Polymeric Biomaterials",

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}

Guler Gokce, Z, Birol, SZ, Mitina, N, Harhay, K, Finiuk, N, Glasunova, V, Stoika, R, Ercelen, S & Zaichenko, A 2021, 'Novel amphiphilic block-copolymer forming stable micelles and interpolyelectrolyte complexes with DNA for efficient gene delivery', International Journal of Polymeric Materials and Polymeric Biomaterials, vol. 70, no. 8, pp. 554-573. https://doi.org/10.1080/00914037.2020.1740988

Novel amphiphilic block-copolymer forming stable micelles and interpolyelectrolyte complexes with DNA for efficient gene delivery. / Guler Gokce, Zeliha; Birol, Semra Zuhal; Mitina, Nataliya et al.
In: International Journal of Polymeric Materials and Polymeric Biomaterials, Vol. 70, No. 8, 2021, p. 554-573.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Novel amphiphilic block-copolymer forming stable micelles and interpolyelectrolyte complexes with DNA for efficient gene delivery

AU - Guler Gokce, Zeliha

AU - Birol, Semra Zuhal

AU - Mitina, Nataliya

AU - Harhay, Khrystyna

AU - Finiuk, Nataliya

AU - Glasunova, Valentina

AU - Stoika, Rostyslav

AU - Ercelen, Sebnem

AU - Zaichenko, Alexander

N1 - Funding Information: The authors express their thanks to TUBITAK, Turkey for the financial support to project numbered 109S258 (SBAG-UKRAYNA-3) and the State Committee of Ukraine for Science and Innovation for funding of the project numbered M/417-2011. Publisher Copyright: © 2020 Taylor & Francis Group, LLC.

PY - 2021

Y1 - 2021

N2 - Novel amphiphilic poly(DMAEMA)-block-poly(NVP-co-BA-co-AEM) (BP83-1) forms stable micelles and BP83-1/pDNA complexes possessing controlled size, charge and enhanced aggregation degree. It was found that the formation of the micelles by BP83-1 is necessary for successful DNA binding and compaction. The polyamphiphile micelle aggregation degree defined their crucial effect on the compaction and morphology of polyplexes. Strong compaction of the DNA upon interaction with polymer at CMC value, positive charge, and high stability of the polyplex are key factors promoting the penetration of DNA through bio-surfaces that define the efficiency of gene delivery in mammalian cells.

AB - Novel amphiphilic poly(DMAEMA)-block-poly(NVP-co-BA-co-AEM) (BP83-1) forms stable micelles and BP83-1/pDNA complexes possessing controlled size, charge and enhanced aggregation degree. It was found that the formation of the micelles by BP83-1 is necessary for successful DNA binding and compaction. The polyamphiphile micelle aggregation degree defined their crucial effect on the compaction and morphology of polyplexes. Strong compaction of the DNA upon interaction with polymer at CMC value, positive charge, and high stability of the polyplex are key factors promoting the penetration of DNA through bio-surfaces that define the efficiency of gene delivery in mammalian cells.

KW - DNA compaction

KW - gene delivery

KW - micelles

KW - polyamphiphile

KW - polyplex formation

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DO - https://doi.org/10.1080/00914037.2020.1740988

M3 - Article

SN - 0091-4037

VL - 70

SP - 554

EP - 573

JO - International Journal of Polymeric Materials and Polymeric Biomaterials

JF - International Journal of Polymeric Materials and Polymeric Biomaterials

IS - 8

ER -

Novel amphiphilic block-copolymer forming stable micelles and interpolyelectrolyte complexes with DNA for efficient gene delivery

Abstract

Keywords

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