Online fluorescent method to assess BCRP/ABCG2 activity in suspension cells

J H Hooijberg; G J Peters; G J L Kaspers; P R Wielinga; A J P Veerman; R Pieters; G Jansen

doi:https://doi.org/10.1081/NCN-200027672

Online fluorescent method to assess BCRP/ABCG2 activity in suspension cells

J H Hooijberg, G J Peters, G J L Kaspers, P R Wielinga, A J P Veerman, R Pieters, G Jansen

Research output: Contribution to journal › Article › Academic › peer-review

5 Citations (Scopus)

Abstract

An online method was developed to monitor BCRP mediated efflux of fluorescent substrates in suspension cells. To this end, a 2-compartment system consisting of a transwell cup and a cuvette was used. In this system we were able to observe differences in efflux kinetics between BCRP overexpressing RPMI 8226/MR cells and parental myeloid RPMI 8226(s) cells using only 50,000 cells per experiment. 8226/MR cells displayed a larger cellular efflux rate of the BCRP substrate Hoechst 33342, as compared to the wildtype cells. This difference in efflux rate was completely decreased in the presence of the BCRP inhibitor Ko143.

Original language	English
Pages (from-to)	1451-4
Number of pages	4
Journal	Nucleosides, Nucleotides and Nucleic Acids
Volume	23
Issue number	8-9
DOIs	https://doi.org/10.1081/NCN-200027672
Publication status	Published - Oct 2004

Keywords

ATP Binding Cassette Transporter, Subfamily G, Member 2
ATP-Binding Cassette Transporters/chemistry
Benzimidazoles/pharmacology
Biological Transport
Cell Line, Tumor
Cells, Cultured
Fluorescent Dyes/pharmacology
Humans
Kinetics
Neoplasm Proteins/chemistry
Radiation-Sensitizing Agents/pharmacology
Software
Spectrometry, Fluorescence/methods
Time Factors

Access to Document

https://doi.org/10.1081/NCN-200027672

Cite this

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title = "Online fluorescent method to assess BCRP/ABCG2 activity in suspension cells",

abstract = "An online method was developed to monitor BCRP mediated efflux of fluorescent substrates in suspension cells. To this end, a 2-compartment system consisting of a transwell cup and a cuvette was used. In this system we were able to observe differences in efflux kinetics between BCRP overexpressing RPMI 8226/MR cells and parental myeloid RPMI 8226(s) cells using only 50,000 cells per experiment. 8226/MR cells displayed a larger cellular efflux rate of the BCRP substrate Hoechst 33342, as compared to the wildtype cells. This difference in efflux rate was completely decreased in the presence of the BCRP inhibitor Ko143.",

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author = "Hooijberg, {J H} and Peters, {G J} and Kaspers, {G J L} and Wielinga, {P R} and Veerman, {A J P} and R Pieters and G Jansen",

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AU - Hooijberg, J H

AU - Peters, G J

AU - Kaspers, G J L

AU - Wielinga, P R

AU - Veerman, A J P

AU - Pieters, R

AU - Jansen, G

PY - 2004/10

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KW - ATP-Binding Cassette Transporters/chemistry

KW - Benzimidazoles/pharmacology

KW - Biological Transport

KW - Cell Line, Tumor

KW - Cells, Cultured

KW - Fluorescent Dyes/pharmacology

KW - Humans

KW - Kinetics

KW - Neoplasm Proteins/chemistry

KW - Radiation-Sensitizing Agents/pharmacology

KW - Software

KW - Spectrometry, Fluorescence/methods

KW - Time Factors

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