TY - JOUR
T1 - Open lung ventilation improves gas exchange and attenuates secondary lung injury in a piglet model of meconium aspiration
AU - Van Kaam, Anton H.
AU - Haitsma, Jack J.
AU - De Jaegere, Anne
AU - Van Aalderen, Wim M.
AU - Kok, Joke H.
AU - Lachmann, Burkhard
PY - 2004/2
Y1 - 2004/2
N2 - Objective: Previous studies failed to show clear benefits of high-frequency ventilation compared with conventional positive pressure ventilation (PPVCON) in experimental meconium aspiration syndrome. However, none of these studies applied an open lung ventilation strategy (OLC), which aims to reduce intrapulmonary shunt due to alveolar collapse. We hypothesized that, if combined with an open lung strategy, both high-frequency oscillatory ventilation (HFOVOLC) and positive pressure ventilation (PPVOLC) would improve gas exchange and attenuate ventilator-induced lung injury in experimental meconium aspiration syndrome. Design: Prospective, randomized animal study. Setting: Research laboratory of a large university. Subjects: Forty-two newborn piglets. Interventions: Thirty minutes after intratracheal meconium instillation, 36 newborn piglets were assigned to one of three ventilation groups - PPVOLC, HFOVOLC, or PPV CON - and ventilated for 5 hrs. In both OLC groups, collapsed alveoli were actively recruited and thereafter stabilized using the lowest possible airway pressures. During PPVCON, ventilator settings were adjusted to prevent critical hypoxia (PaO2 <60 torr [8 kPa]). Six animals served as saline controls. Measurements and Main Results: Compared with the PPVCON group, arterial oxygenation and lung mechanics were superior in both OLC groups and the saline controls. Analysis of the bronchoalveolar lavage fluid obtained after 5 hrs of ventilation showed increased myeloperoxidase activity in the PPVCON group compared with both OLC groups and saline controls. Alveolar protein influx was not different between the groups. Histologic analysis revealed a higher lung injury score in the PPVCON group compared with the PPVOLC and the HFOVOLC groups. Conclusions: Application of the OLC during PPV and HFOV is feasible in experimental meconium aspiration syndrome and results in superior oxygenation and less ventilator-induced lung injury compared with PPVCON.
AB - Objective: Previous studies failed to show clear benefits of high-frequency ventilation compared with conventional positive pressure ventilation (PPVCON) in experimental meconium aspiration syndrome. However, none of these studies applied an open lung ventilation strategy (OLC), which aims to reduce intrapulmonary shunt due to alveolar collapse. We hypothesized that, if combined with an open lung strategy, both high-frequency oscillatory ventilation (HFOVOLC) and positive pressure ventilation (PPVOLC) would improve gas exchange and attenuate ventilator-induced lung injury in experimental meconium aspiration syndrome. Design: Prospective, randomized animal study. Setting: Research laboratory of a large university. Subjects: Forty-two newborn piglets. Interventions: Thirty minutes after intratracheal meconium instillation, 36 newborn piglets were assigned to one of three ventilation groups - PPVOLC, HFOVOLC, or PPV CON - and ventilated for 5 hrs. In both OLC groups, collapsed alveoli were actively recruited and thereafter stabilized using the lowest possible airway pressures. During PPVCON, ventilator settings were adjusted to prevent critical hypoxia (PaO2 <60 torr [8 kPa]). Six animals served as saline controls. Measurements and Main Results: Compared with the PPVCON group, arterial oxygenation and lung mechanics were superior in both OLC groups and the saline controls. Analysis of the bronchoalveolar lavage fluid obtained after 5 hrs of ventilation showed increased myeloperoxidase activity in the PPVCON group compared with both OLC groups and saline controls. Alveolar protein influx was not different between the groups. Histologic analysis revealed a higher lung injury score in the PPVCON group compared with the PPVOLC and the HFOVOLC groups. Conclusions: Application of the OLC during PPV and HFOV is feasible in experimental meconium aspiration syndrome and results in superior oxygenation and less ventilator-induced lung injury compared with PPVCON.
KW - High-frequency ventilation
KW - Histology
KW - Intermittent positive pressure ventilation
KW - Meconium aspiration
KW - Respiratory mechanics
KW - Ventilator-induced lung injury
UR - http://www.scopus.com/inward/record.url?scp=1242342726&partnerID=8YFLogxK
U2 - https://doi.org/10.1097/01.CCM.0000104952.61111.49
DO - https://doi.org/10.1097/01.CCM.0000104952.61111.49
M3 - Article
C2 - 14758162
SN - 0090-3493
VL - 32
SP - 443
EP - 449
JO - Critical Care Medicine
JF - Critical Care Medicine
IS - 2
ER -