TY - JOUR
T1 - Optical coherence tomography
AU - Bouma, Brett E.
AU - de Boer, Johannes F.
AU - Huang, David
AU - Jang, Ik Kyung
AU - Yonetsu, Taishi
AU - Leggett, Cadman L.
AU - Leitgeb, Rainer
AU - Sampson, David D.
AU - Suter, Melissa
AU - Vakoc, Ben J.
AU - Villiger, Martin
AU - Wojtkowski, Maciej
N1 - Funding Information: B.E.B., J.F.d.B., B.J.V. and M.V. are inventors on patents owned by Mass General Brigham in the field of optical coherence tomography (OCT) and acknowledge patent royalties, administered through Mass General Brigham, from organizations that may gain or lose financially through this publication. I.-K.J. has received educational grants from Abbott Vascular and consulting fees from Svelte Medical Systems, Inc. and Mitobridge, Inc. D.H. and Oregon Health & Science University (OHSU) have significant financial interests in an organization that may gain or lose financially through this publication. D.H. acknowledges research support and patent royalty from an organization that may gain or lose financially through this publication. D.D.S. is an inventor on patents owned by the University of Western Australia in the field of OCT and licensed to organizations that may gain or lose financially through this publication. T.Y., C.L.L., R.L., M.S. and M.W. declare no competing interests. Publisher Copyright: © 2022, Springer Nature Limited.
PY - 2022/10/13
Y1 - 2022/10/13
N2 - Optical coherence tomography (OCT) is a non-contact method for imaging the topological and internal microstructure of samples in three dimensions. OCT can be configured as a conventional microscope, an ophthalmic scanner or endoscopes and small-diameter catheters for accessing internal biological organs. In this Primer, the principles underpinning the different instrument configurations that are tailored to distinct imaging applications are described and the origin of signal, based on light scattering and propagation, is explained. Although OCT has been used for imaging inanimate objects, the discussion focuses on biological and medical imaging. The signal processing methods and algorithms that make OCT exquisitely sensitive to reflections, as weak as just a few photons, and reveal functional information in addition to structure are examined. Image processing, display and interpretation, which are all critical for effective biomedical imaging, are discussed in the context of specific applications. Finally, image artefacts and limitations that commonly arise and future advances and opportunities are considered.
AB - Optical coherence tomography (OCT) is a non-contact method for imaging the topological and internal microstructure of samples in three dimensions. OCT can be configured as a conventional microscope, an ophthalmic scanner or endoscopes and small-diameter catheters for accessing internal biological organs. In this Primer, the principles underpinning the different instrument configurations that are tailored to distinct imaging applications are described and the origin of signal, based on light scattering and propagation, is explained. Although OCT has been used for imaging inanimate objects, the discussion focuses on biological and medical imaging. The signal processing methods and algorithms that make OCT exquisitely sensitive to reflections, as weak as just a few photons, and reveal functional information in addition to structure are examined. Image processing, display and interpretation, which are all critical for effective biomedical imaging, are discussed in the context of specific applications. Finally, image artefacts and limitations that commonly arise and future advances and opportunities are considered.
UR - http://www.scopus.com/inward/record.url?scp=85139992292&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85139992292&partnerID=8YFLogxK
U2 - https://doi.org/10.1038/s43586-022-00162-2
DO - https://doi.org/10.1038/s43586-022-00162-2
M3 - Article
C2 - 36751306
SN - 2662-8449
VL - 2
JO - Nature Reviews Methods Primers
JF - Nature Reviews Methods Primers
IS - 1
M1 - 79
ER -