Optimization of real-time PCR assay for rapid and sensitive detection of eubacterial 16S ribosomal DNA in platelet concentrates.

T. Mohammadi; H.W. Reesink; C.M.J.E. Vandenbroucke-Grauls; P.H.M. Savelkoul

doi:https://doi.org/10.1128/JCM.41.10.4796-4798.2003

Optimization of real-time PCR assay for rapid and sensitive detection of eubacterial 16S ribosomal DNA in platelet concentrates.

T. Mohammadi, H.W. Reesink, C.M.J.E. Vandenbroucke-Grauls, P.H.M. Savelkoul

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

A real-time PCR assay was developed for rapid detection of eubacterial 16S ribosomal DNA in platelet concentrates. The sensitivity of this assay can be hampered by contaminating DNA in the PCR reagents. Digestion of the PCR reagents with Sau3AI prior to PCR amplification was effective in eliminating this contaminating DNA without affecting the sensitivity of the assay

Original language	English
Pages (from-to)	4796-8
Journal	Journal of clinical microbiology
Volume	41
Issue number	10
DOIs	https://doi.org/10.1128/JCM.41.10.4796-4798.2003
Publication status	Published - 2003

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https://doi.org/10.1128/JCM.41.10.4796-4798.2003

https://research.vumc.nl/ws/files/10001204/PMC254335

Cite this

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title = "Optimization of real-time PCR assay for rapid and sensitive detection of eubacterial 16S ribosomal DNA in platelet concentrates.",

abstract = "A real-time PCR assay was developed for rapid detection of eubacterial 16S ribosomal DNA in platelet concentrates. The sensitivity of this assay can be hampered by contaminating DNA in the PCR reagents. Digestion of the PCR reagents with Sau3AI prior to PCR amplification was effective in eliminating this contaminating DNA without affecting the sensitivity of the assay",

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AU - Savelkoul, P.H.M.

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AB - A real-time PCR assay was developed for rapid detection of eubacterial 16S ribosomal DNA in platelet concentrates. The sensitivity of this assay can be hampered by contaminating DNA in the PCR reagents. Digestion of the PCR reagents with Sau3AI prior to PCR amplification was effective in eliminating this contaminating DNA without affecting the sensitivity of the assay

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