Overexpression of the human major vault protein in gangliogliomas.

E Aronica; J.A. Gorter; EA van Vliet; WG Spliet; CW van Veelen; PC van Rijen; S. Leenstra; MD Ramkema; G.L. Scheffer; R.J. Scheper; SM Sisodiya; D. Troost; Erwin A. van Vliet; Wim G. M. Spliet; Cees W. M. van Veelen; Peter C. van Rijen; Marja D. Ramkema; Sanjay M. Sisodiya

doi:https://doi.org/10.1046/j.1528-1157.2003.22103.x

Overexpression of the human major vault protein in gangliogliomas.

E Aronica, J.A. Gorter, EA van Vliet, WG Spliet, CW van Veelen, PC van Rijen, S. Leenstra, MD Ramkema, G.L. Scheffer, R.J. Scheper, SM Sisodiya, D. Troost, Erwin A. van Vliet, Wim G. M. Spliet, Cees W. M. van Veelen, Peter C. van Rijen, Marja D. Ramkema, Sanjay M. Sisodiya

Research output: Contribution to journal › Article › Academic › peer-review

30 Citations (Scopus)

Abstract

Purpose: Recent evidence has been obtained that the major vault protein (MVP) may play a role in multidrug resistance (MDR). We investigated the expression and cellular localization of MVP in gangliogliomas (GGs), which are increasingly recognized causes of chronic pharmacoresistant epilepsy. Methods: Surgical tumor specimens (n = 30), as well as peri-tumoral and control brain tissues, were examined for the cellular distribution pattern of MVP with immunocytochemistry. Western blot analysis showed a consistent increase in MVP expression in GGs compared with that in control cortex. Results: In normal brain, MVP expression was below detection in glial and neuronal cells, and only low immunoreactivity (IR) levels were detected in blood vessels. MVP expression was observed in the neuronal component of 30 of 30 GGs and in a population of tumor glial cells. In the majority of the tumors, strong MVP IR was found in lesional vessels. Perilesional regions did not show increased staining in vessels or in neuronal and glial cells compared with normal cortex. However, expression of MVP was detected in the hippocampus in cases with dual pathology. Conclusions: The increased expression of MVP in GGs is another example of an MDR-related protein that is upregulated in patients with refractory epilepsy. Further research is necessary to investigate whether it could play role in the mechanisms underlying drug resistance in chronic human epilepsy

Original language	English
Pages (from-to)	1166-75
Journal	Epilepsia
Volume	44
Issue number	9
DOIs	https://doi.org/10.1046/j.1528-1157.2003.22103.x
Publication status	Published - 2003

Keywords

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https://pure.uva.nl/ws/files/3427154/26294_119525n.pdf

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Aronica, E., Gorter, J. A., van Vliet, EA., Spliet, WG., van Veelen, CW., van Rijen, PC., Leenstra, S., Ramkema, MD., Scheffer, G. L., Scheper, R. J., Sisodiya, SM., Troost, D., van Vliet, E. A., Spliet, W. G. M., van Veelen, C. W. M., van Rijen, P. C., Ramkema, M. D., & Sisodiya, S. M. (2003). Overexpression of the human major vault protein in gangliogliomas. Epilepsia, 44(9), 1166-75. https://doi.org/10.1046/j.1528-1157.2003.22103.x

@article{1cadcf9394d94a419382f09833d4b7b6,

title = "Overexpression of the human major vault protein in gangliogliomas.",

abstract = "Purpose: Recent evidence has been obtained that the major vault protein (MVP) may play a role in multidrug resistance (MDR). We investigated the expression and cellular localization of MVP in gangliogliomas (GGs), which are increasingly recognized causes of chronic pharmacoresistant epilepsy. Methods: Surgical tumor specimens (n = 30), as well as peri-tumoral and control brain tissues, were examined for the cellular distribution pattern of MVP with immunocytochemistry. Western blot analysis showed a consistent increase in MVP expression in GGs compared with that in control cortex. Results: In normal brain, MVP expression was below detection in glial and neuronal cells, and only low immunoreactivity (IR) levels were detected in blood vessels. MVP expression was observed in the neuronal component of 30 of 30 GGs and in a population of tumor glial cells. In the majority of the tumors, strong MVP IR was found in lesional vessels. Perilesional regions did not show increased staining in vessels or in neuronal and glial cells compared with normal cortex. However, expression of MVP was detected in the hippocampus in cases with dual pathology. Conclusions: The increased expression of MVP in GGs is another example of an MDR-related protein that is upregulated in patients with refractory epilepsy. Further research is necessary to investigate whether it could play role in the mechanisms underlying drug resistance in chronic human epilepsy",

keywords = "AMC wi-eigen",

author = "E Aronica and J.A. Gorter and {van Vliet}, EA and WG Spliet and {van Veelen}, CW and {van Rijen}, PC and S. Leenstra and MD Ramkema and G.L. Scheffer and R.J. Scheper and SM Sisodiya and D. Troost and {van Vliet}, {Erwin A.} and Spliet, {Wim G. M.} and {van Veelen}, {Cees W. M.} and {van Rijen}, {Peter C.} and Ramkema, {Marja D.} and Sisodiya, {Sanjay M.}",

year = "2003",

doi = "https://doi.org/10.1046/j.1528-1157.2003.22103.x",

language = "English",

volume = "44",

pages = "1166--75",

journal = "Epilepsia",

issn = "0013-9580",

publisher = "Wiley-Blackwell",

number = "9",

}

Aronica, E, Gorter, JA, van Vliet, EA, Spliet, WG, van Veelen, CW, van Rijen, PC, Leenstra, S, Ramkema, MD, Scheffer, GL, Scheper, RJ, Sisodiya, SM, Troost, D, van Vliet, EA, Spliet, WGM, van Veelen, CWM, van Rijen, PC, Ramkema, MD & Sisodiya, SM 2003, 'Overexpression of the human major vault protein in gangliogliomas.', Epilepsia, vol. 44, no. 9, pp. 1166-75. https://doi.org/10.1046/j.1528-1157.2003.22103.x

TY - JOUR

T1 - Overexpression of the human major vault protein in gangliogliomas.

AU - Aronica, E

AU - Gorter, J.A.

AU - van Vliet, EA

AU - Spliet, WG

AU - van Veelen, CW

AU - van Rijen, PC

AU - Leenstra, S.

AU - Ramkema, MD

AU - Scheffer, G.L.

AU - Scheper, R.J.

AU - Sisodiya, SM

AU - Troost, D.

AU - van Vliet, Erwin A.

AU - Spliet, Wim G. M.

AU - van Veelen, Cees W. M.

AU - van Rijen, Peter C.

AU - Ramkema, Marja D.

AU - Sisodiya, Sanjay M.

PY - 2003

Y1 - 2003

N2 - Purpose: Recent evidence has been obtained that the major vault protein (MVP) may play a role in multidrug resistance (MDR). We investigated the expression and cellular localization of MVP in gangliogliomas (GGs), which are increasingly recognized causes of chronic pharmacoresistant epilepsy. Methods: Surgical tumor specimens (n = 30), as well as peri-tumoral and control brain tissues, were examined for the cellular distribution pattern of MVP with immunocytochemistry. Western blot analysis showed a consistent increase in MVP expression in GGs compared with that in control cortex. Results: In normal brain, MVP expression was below detection in glial and neuronal cells, and only low immunoreactivity (IR) levels were detected in blood vessels. MVP expression was observed in the neuronal component of 30 of 30 GGs and in a population of tumor glial cells. In the majority of the tumors, strong MVP IR was found in lesional vessels. Perilesional regions did not show increased staining in vessels or in neuronal and glial cells compared with normal cortex. However, expression of MVP was detected in the hippocampus in cases with dual pathology. Conclusions: The increased expression of MVP in GGs is another example of an MDR-related protein that is upregulated in patients with refractory epilepsy. Further research is necessary to investigate whether it could play role in the mechanisms underlying drug resistance in chronic human epilepsy

AB - Purpose: Recent evidence has been obtained that the major vault protein (MVP) may play a role in multidrug resistance (MDR). We investigated the expression and cellular localization of MVP in gangliogliomas (GGs), which are increasingly recognized causes of chronic pharmacoresistant epilepsy. Methods: Surgical tumor specimens (n = 30), as well as peri-tumoral and control brain tissues, were examined for the cellular distribution pattern of MVP with immunocytochemistry. Western blot analysis showed a consistent increase in MVP expression in GGs compared with that in control cortex. Results: In normal brain, MVP expression was below detection in glial and neuronal cells, and only low immunoreactivity (IR) levels were detected in blood vessels. MVP expression was observed in the neuronal component of 30 of 30 GGs and in a population of tumor glial cells. In the majority of the tumors, strong MVP IR was found in lesional vessels. Perilesional regions did not show increased staining in vessels or in neuronal and glial cells compared with normal cortex. However, expression of MVP was detected in the hippocampus in cases with dual pathology. Conclusions: The increased expression of MVP in GGs is another example of an MDR-related protein that is upregulated in patients with refractory epilepsy. Further research is necessary to investigate whether it could play role in the mechanisms underlying drug resistance in chronic human epilepsy

KW - AMC wi-eigen

U2 - https://doi.org/10.1046/j.1528-1157.2003.22103.x

DO - https://doi.org/10.1046/j.1528-1157.2003.22103.x

M3 - Article

C2 - 12919388

SN - 0013-9580

VL - 44

SP - 1166

EP - 1175

JO - Epilepsia

JF - Epilepsia

IS - 9

ER -