TY - JOUR
T1 - Pairing Bacteroides vulgatus LPS Structure with Its Immunomodulatory Effects on Human Cellular Models
AU - Di Lorenzo, Flaviana
AU - Pither, Molly D.
AU - Martufi, Michela
AU - Scarinci, Ilaria
AU - Guzman-Caldentey, Joan
AU - Lakomiec, Ewelina
AU - Jachymek, Wojciech
AU - Bruijns, Sven C. M.
AU - Martin Santamaria, Sonsoles
AU - Frick, Julia-Stephanie
AU - van Kooyk, Yvette
AU - Chiodo, Fabrizio
AU - Silipo, Alba
AU - Bernardini, Maria Lina
AU - Molinaro, Antonio
AU - Łakomiec, Ewelina
N1 - Funding Information: F.D.L. acknowledges Progetto STAR 2018 Linea 1 grant E66C18001330003. S.M.S. acknowledges Spanish Ministry of Science (ref. CTQ2017-88353-R). A.M., F.D.L., and A.S. acknowledge H2020 Marie Sklodowska-Curie ITN 2018 "SweetCrossTalk" grant 814102. A.M. acknowledges progetto POR SATIN POR-FESR 2 0 1 4 - 2 0 2 0 g r a n t B61C17000070007 (OR3) and Progetto POR Campania Oncoterapia 2014-2020 grant B61G18000470007. A.S. acknowledges PRIN-MIUR 2017 Glytunes project. A.S. and F.C. acknowledge COST (European Cooperation in Science and Technology) Action CA18103 (INNOGLY). F.C. was financially supported by the NWO Spinoza award of Y.K. Funding Information: F.D.L. acknowledges Progetto STAR 2018 Linea 1 grant E66C18001330003. S.M.S. acknowledges Spanish Ministry of Science (ref. CTQ2017-88353-R). A.M., F.D.L., and A.S. acknowledge H2020 Marie Skłodowska-Curie ITN 2018 “SweetCrossTalk” grant 814102. A.M. acknowledges progetto POR SATIN POR-FESR 2014–2020 grant B61C17000070007 (OR3) and Progetto POR Campania Oncoterapia 2014–2020 grant B61G18000470007. A.S. acknowledges PRIN-MIUR 2017 Glytunes project. A.S. and F.C. acknowledge COST (European Cooperation in Science and Technology) Action CA18103 (INNOGLY). F.C. was financially supported by the NWO Spinoza award of Y.K. Publisher Copyright: Copyright © 2020 American Chemical Society. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/9/23
Y1 - 2020/9/23
N2 - The gut microbiota guide the development of the host immune system by setting a systemic threshold for immune activation. Lipopolysaccharides (LPSs) from gut bacteria are able to trigger systemic and local proinflammatory and immunomodulatory responses, and this capability strongly relies on their fine structures. Up to now, only a few LPS structures from gut commensals have been elucidated; therefore, the molecular motifs that may be important for LPS-mammalian cell interactions at the gut level are still obscure. Here, we report on the full structure of the LPS isolated from one of the prominent species of the genus Bacteroides, Bacteroides vulgatus. The LPS turned out to consist of a particular chemical structure based on hypoacylated and mono-phosphorylated lipid A and with a galactofuranose-containing core oligosaccharide and an O-antigen built up of mannose and rhamnose. The evaluation of the immunological properties of this LPS on human in vitro models revealed a very interesting capability to produce anti-inflammatory cytokines and to induce a synergistic action of MD-2/TLR4- and TLR2-mediated signaling pathways.
AB - The gut microbiota guide the development of the host immune system by setting a systemic threshold for immune activation. Lipopolysaccharides (LPSs) from gut bacteria are able to trigger systemic and local proinflammatory and immunomodulatory responses, and this capability strongly relies on their fine structures. Up to now, only a few LPS structures from gut commensals have been elucidated; therefore, the molecular motifs that may be important for LPS-mammalian cell interactions at the gut level are still obscure. Here, we report on the full structure of the LPS isolated from one of the prominent species of the genus Bacteroides, Bacteroides vulgatus. The LPS turned out to consist of a particular chemical structure based on hypoacylated and mono-phosphorylated lipid A and with a galactofuranose-containing core oligosaccharide and an O-antigen built up of mannose and rhamnose. The evaluation of the immunological properties of this LPS on human in vitro models revealed a very interesting capability to produce anti-inflammatory cytokines and to induce a synergistic action of MD-2/TLR4- and TLR2-mediated signaling pathways.
UR - http://www.scopus.com/inward/record.url?scp=85089466810&partnerID=8YFLogxK
U2 - https://doi.org/10.1021/acscentsci.0c00791
DO - https://doi.org/10.1021/acscentsci.0c00791
M3 - Article
C2 - 32999936
SN - 2374-7943
VL - 6
SP - 1602
EP - 1616
JO - ACS central science
JF - ACS central science
IS - 9
ER -