TY - JOUR
T1 - Peach allergen Pru p 1 content is generally low in fruit but with large variation in different varieties
AU - Jin, Jing
AU - Gan, Kexin
AU - Zhao, Lan
AU - Jia, Huijuan
AU - Zhu, Yifan
AU - Li, Xiongwei
AU - Yang, Zhaowei
AU - Ye, Zhengwen
AU - Cao, Ke
AU - Wang, Zhiqiang
AU - Yu, Mingliang
AU - Zhang, Yuyan
AU - Ma, Zhisheng
AU - Liu, Hangkong
AU - Arús, Pere
AU - Akkerdaas, Jaap H.
AU - Gao, Zhongshan
AU - van Ree, Ronald
N1 - Funding Information: The authors thank Dr. Shen Zhijun, Dr. Niu Liang, and Ms Chen Miaojin for providing some peach varieties, Dr. Eric van de Weg for providing rMal d 1 allergen. This study was funded by The Key Project for New Agricultural Cultivar Breeding in Zhejiang Province, China (2016C02052‐5), Shanghai Science and Technology Committee Rising‐Star Program (19QB1404600). Funding Information: The authors thank Dr. Shen Zhijun, Dr. Niu Liang, and Ms Chen Miaojin for providing some peach varieties, Dr. Eric van de Weg for providing rMal d 1 allergen. This study was funded by The Key Project for New Agricultural Cultivar Breeding in Zhejiang Province, China (2016C02052-5), Shanghai Science and Technology Committee Rising-Star Program (19QB1404600). Publisher Copyright: © 2021 The Authors. Clinical and Translational Allergy published by John Wiley and Sons Ltd on behalf of European Academy of Allergy and Clinical Immunology.
PY - 2021/5/1
Y1 - 2021/5/1
N2 - Background: Pru p 1 is a major allergen in peach and nectarine, and the different content in varieties may affect the degree of allergic reactions. This study aimed to quantify Pru p 1 levels in representative peach varieties and select hypoallergenic Pru p 1 varieties. Methods: To obtain monoclonal and polyclonal antibodies, mice and rabbits, respectively, were immunized with recombinant Pru p 1.01 and Pru p 1.02. The Pru p 1 levels in fruits from 83 representative peach varieties was quantified by sandwich enzyme-linked immunosorbent assay (sELISA). nPru p 1 was obtained through specific monoclonal antibody affinity purification and confirmed by Western blot and mass spectrometry. The variable Pru p 1 content of selected varieties was evaluated by Western blot and the expression level of encoding Pru p 1 genes by quantitative polymerase chain reaction. Results: A sELISA method with monoclonal and polyclonal antibodies was built for quantifying Pru p 1 levels in peach. Pru p 1 was mainly concentrated in the peel (0.20–73.44 μg/g, fresh weight), being very low in the pulp (0.05–9.62 μg/g) and not detected in wild peach. For the 78 peach and nectarine varieties, Pru p 1 content varied widely from 0.12 to 6.45 μg/g in whole fruit. We verified that natural Pru p 1 is composed of 1.01 and 1.02 isoallergens, and the Pru p 1 expression level and Pru p 1 band intensity in the immunoblots were in agreement with protein quantity determined by ELISA for some tested varieties. In some cases, the reduced levels of Pru p 1 did not coincide with low Pru p 3 in the same variety in whole fruit, while some ancient wild peach and nectarines contained low levels of both allergens, and late-ripening yellow flesh varieties were usually highly allergenic. Conclusion: Pru p 1 content is generally low in peach compared to Pru p 3. Several hypoallergenic Pru p 1 and Pru p 3 varieties, “Zi Xue Tao,” “Wu Yue Xian,” and “May Fire,” were identified, which could be useful in trials for peach allergy patients.
AB - Background: Pru p 1 is a major allergen in peach and nectarine, and the different content in varieties may affect the degree of allergic reactions. This study aimed to quantify Pru p 1 levels in representative peach varieties and select hypoallergenic Pru p 1 varieties. Methods: To obtain monoclonal and polyclonal antibodies, mice and rabbits, respectively, were immunized with recombinant Pru p 1.01 and Pru p 1.02. The Pru p 1 levels in fruits from 83 representative peach varieties was quantified by sandwich enzyme-linked immunosorbent assay (sELISA). nPru p 1 was obtained through specific monoclonal antibody affinity purification and confirmed by Western blot and mass spectrometry. The variable Pru p 1 content of selected varieties was evaluated by Western blot and the expression level of encoding Pru p 1 genes by quantitative polymerase chain reaction. Results: A sELISA method with monoclonal and polyclonal antibodies was built for quantifying Pru p 1 levels in peach. Pru p 1 was mainly concentrated in the peel (0.20–73.44 μg/g, fresh weight), being very low in the pulp (0.05–9.62 μg/g) and not detected in wild peach. For the 78 peach and nectarine varieties, Pru p 1 content varied widely from 0.12 to 6.45 μg/g in whole fruit. We verified that natural Pru p 1 is composed of 1.01 and 1.02 isoallergens, and the Pru p 1 expression level and Pru p 1 band intensity in the immunoblots were in agreement with protein quantity determined by ELISA for some tested varieties. In some cases, the reduced levels of Pru p 1 did not coincide with low Pru p 3 in the same variety in whole fruit, while some ancient wild peach and nectarines contained low levels of both allergens, and late-ripening yellow flesh varieties were usually highly allergenic. Conclusion: Pru p 1 content is generally low in peach compared to Pru p 3. Several hypoallergenic Pru p 1 and Pru p 3 varieties, “Zi Xue Tao,” “Wu Yue Xian,” and “May Fire,” were identified, which could be useful in trials for peach allergy patients.
KW - ELISA
KW - Pru p 1
KW - hypoallergenic varieties
KW - peach allergy
KW - protein quantification
UR - http://www.scopus.com/inward/record.url?scp=85106928702&partnerID=8YFLogxK
U2 - https://doi.org/10.1002/clt2.12034
DO - https://doi.org/10.1002/clt2.12034
M3 - Article
C2 - 34025984
SN - 2045-7022
VL - 11
JO - Clinical and Translational Allergy
JF - Clinical and Translational Allergy
IS - 3
M1 - e12034
ER -