Performance of a prototype malaria rapid diagnostic test versus thick film microscopy among HIV-positive subjects in rural Rakai, Uganda

Lisa A. Mills; Joseph Kagaayi; Joseph P. Shott; Kevin Newell; John Baptist Bwanika; Victor Ssempijja; Simon Aluma; Thomas C. Quinn; Steven J. Reynolds; Ronald H. Gray

doi:https://doi.org/10.1016/j.trstmh.2009.07.030

Performance of a prototype malaria rapid diagnostic test versus thick film microscopy among HIV-positive subjects in rural Rakai, Uganda

Lisa A. Mills, Joseph Kagaayi, Joseph P. Shott, Kevin Newell, John Baptist Bwanika, Victor Ssempijja, Simon Aluma, Thomas C. Quinn, Steven J. Reynolds, Ronald H. Gray

Research output: Contribution to journal › Article › Academic › peer-review

10 Citations (Scopus)

Abstract

In this study, we report the performance of a prototype malaria rapid diagnostic test, Malaria F-test (MFT), compared with thick blood films from HIV-positive Ugandans undergoing malaria testing. In total, 21/154 samples (13.6%) were concordantly positive by both thick film and MFT and 129/154 samples (83.8%) were concordantly negative; 1 sample (0.6%) was thick film-positive but MFT-negative and 3 samples (1.9%) were thick film-negative but MFT-positive. The sensitivity of MFT was 95.5% (95% CI 77.2-99.9%) compared with thick film microscopy and the specificity was 97.7% (95% CI 93.5-99.5%). MFT was simple, rapid and effective for detection of Plasmodium falciparum among HIV-positive subjects in a rural, malaria-endemic African setting.

Original language	English
Pages (from-to)	237-239
Number of pages	3
Journal	Transactions of the Royal Society of Tropical Medicine and Hygiene
Volume	104
Issue number	3
DOIs	https://doi.org/10.1016/j.trstmh.2009.07.030
Publication status	Published - Mar 2010

Keywords

Africa
Diagnosis
HIV/AIDS
Malaria
Rapid diagnostic test
Uganda

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Mills, L. A., Kagaayi, J., Shott, J. P., Newell, K., Bwanika, J. B., Ssempijja, V., Aluma, S., Quinn, T. C., Reynolds, S. J., & Gray, R. H. (2010). Performance of a prototype malaria rapid diagnostic test versus thick film microscopy among HIV-positive subjects in rural Rakai, Uganda. Transactions of the Royal Society of Tropical Medicine and Hygiene, 104(3), 237-239. https://doi.org/10.1016/j.trstmh.2009.07.030

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title = "Performance of a prototype malaria rapid diagnostic test versus thick film microscopy among HIV-positive subjects in rural Rakai, Uganda",

abstract = "In this study, we report the performance of a prototype malaria rapid diagnostic test, Malaria F-test (MFT), compared with thick blood films from HIV-positive Ugandans undergoing malaria testing. In total, 21/154 samples (13.6%) were concordantly positive by both thick film and MFT and 129/154 samples (83.8%) were concordantly negative; 1 sample (0.6%) was thick film-positive but MFT-negative and 3 samples (1.9%) were thick film-negative but MFT-positive. The sensitivity of MFT was 95.5% (95% CI 77.2-99.9%) compared with thick film microscopy and the specificity was 97.7% (95% CI 93.5-99.5%). MFT was simple, rapid and effective for detection of Plasmodium falciparum among HIV-positive subjects in a rural, malaria-endemic African setting.",

keywords = "Africa, Diagnosis, HIV/AIDS, Malaria, Rapid diagnostic test, Uganda",

author = "Mills, {Lisa A.} and Joseph Kagaayi and Shott, {Joseph P.} and Kevin Newell and Bwanika, {John Baptist} and Victor Ssempijja and Simon Aluma and Quinn, {Thomas C.} and Reynolds, {Steven J.} and Gray, {Ronald H.}",

note = "Funding Information: This project was funded in whole or in part with federal funds from the Division of Intramural Research, National Institute of Allergy and Infectious Diseases, and the National Cancer Institute, National Institutes of Health, under Contract Number HHSN261200800001E. Research support and test kits were provided by BD Diagnostics (Sparks, MD, USA).",

year = "2010",

month = mar,

doi = "https://doi.org/10.1016/j.trstmh.2009.07.030",

language = "English",

volume = "104",

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Mills, LA, Kagaayi, J, Shott, JP, Newell, K, Bwanika, JB, Ssempijja, V, Aluma, S, Quinn, TC, Reynolds, SJ & Gray, RH 2010, 'Performance of a prototype malaria rapid diagnostic test versus thick film microscopy among HIV-positive subjects in rural Rakai, Uganda', Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 104, no. 3, pp. 237-239. https://doi.org/10.1016/j.trstmh.2009.07.030

Performance of a prototype malaria rapid diagnostic test versus thick film microscopy among HIV-positive subjects in rural Rakai, Uganda. / Mills, Lisa A.; Kagaayi, Joseph; Shott, Joseph P. et al.
In: Transactions of the Royal Society of Tropical Medicine and Hygiene, Vol. 104, No. 3, 03.2010, p. 237-239.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Performance of a prototype malaria rapid diagnostic test versus thick film microscopy among HIV-positive subjects in rural Rakai, Uganda

AU - Mills, Lisa A.

AU - Kagaayi, Joseph

AU - Shott, Joseph P.

AU - Newell, Kevin

AU - Bwanika, John Baptist

AU - Ssempijja, Victor

AU - Aluma, Simon

AU - Quinn, Thomas C.

AU - Reynolds, Steven J.

AU - Gray, Ronald H.

N1 - Funding Information: This project was funded in whole or in part with federal funds from the Division of Intramural Research, National Institute of Allergy and Infectious Diseases, and the National Cancer Institute, National Institutes of Health, under Contract Number HHSN261200800001E. Research support and test kits were provided by BD Diagnostics (Sparks, MD, USA).

PY - 2010/3

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N2 - In this study, we report the performance of a prototype malaria rapid diagnostic test, Malaria F-test (MFT), compared with thick blood films from HIV-positive Ugandans undergoing malaria testing. In total, 21/154 samples (13.6%) were concordantly positive by both thick film and MFT and 129/154 samples (83.8%) were concordantly negative; 1 sample (0.6%) was thick film-positive but MFT-negative and 3 samples (1.9%) were thick film-negative but MFT-positive. The sensitivity of MFT was 95.5% (95% CI 77.2-99.9%) compared with thick film microscopy and the specificity was 97.7% (95% CI 93.5-99.5%). MFT was simple, rapid and effective for detection of Plasmodium falciparum among HIV-positive subjects in a rural, malaria-endemic African setting.

AB - In this study, we report the performance of a prototype malaria rapid diagnostic test, Malaria F-test (MFT), compared with thick blood films from HIV-positive Ugandans undergoing malaria testing. In total, 21/154 samples (13.6%) were concordantly positive by both thick film and MFT and 129/154 samples (83.8%) were concordantly negative; 1 sample (0.6%) was thick film-positive but MFT-negative and 3 samples (1.9%) were thick film-negative but MFT-positive. The sensitivity of MFT was 95.5% (95% CI 77.2-99.9%) compared with thick film microscopy and the specificity was 97.7% (95% CI 93.5-99.5%). MFT was simple, rapid and effective for detection of Plasmodium falciparum among HIV-positive subjects in a rural, malaria-endemic African setting.

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M3 - Article

C2 - 19765788

SN - 0035-9203

VL - 104

SP - 237

EP - 239

JO - Transactions of the Royal Society of Tropical Medicine and Hygiene

JF - Transactions of the Royal Society of Tropical Medicine and Hygiene

IS - 3

ER -

Performance of a prototype malaria rapid diagnostic test versus thick film microscopy among HIV-positive subjects in rural Rakai, Uganda

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Keywords

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