TY - JOUR
T1 - Peri-implant tissue is an important niche for Staphylococcus epidermidis in experimental biomaterial-associated infection in mice
AU - Broekhuizen, Corine A. N.
AU - de Boer, Leonie
AU - Schipper, Kim
AU - Jones, Christopher D.
AU - Quadir, Shan
AU - Feldman, Roger G.
AU - Dankert, Jacob
AU - Vandenbroucke-Grauls, Christina M. J. E.
AU - Weening, Jan J.
AU - Zaat, Sebastian A. J.
PY - 2007
Y1 - 2007
N2 - Biomaterial-associated infections (BAI), which are predominantly caused by Staphylococcus epidermidis, are a significant problem in modern medicine. Biofilm formation is considered the pivotal element in the pathogenesis, but in previous mouse studies we retrieved S. epidermidis from peri-implant tissue. To assess the kinetics and generality of tissue colonization, we investigated BAI using two S. epidermidis strains, two biomaterials, and two mouse strains. With small inocula all implants were culture negative, whereas surrounding tissues were positive. When higher doses were used, tissues were culture positive more often than implants, with higher numbers of CFU. This was true for the different biomaterials tested, for both S. epidermidis strains, at different times, and for both mouse strains. S. epidermidis colocalized with host cells at a distance that was >10 cell layers from the biomaterial-tissue interface. We concluded that in mouse experimental BAI S. epidermidis peri-implant tissue colonization is more important than biofilm formation
AB - Biomaterial-associated infections (BAI), which are predominantly caused by Staphylococcus epidermidis, are a significant problem in modern medicine. Biofilm formation is considered the pivotal element in the pathogenesis, but in previous mouse studies we retrieved S. epidermidis from peri-implant tissue. To assess the kinetics and generality of tissue colonization, we investigated BAI using two S. epidermidis strains, two biomaterials, and two mouse strains. With small inocula all implants were culture negative, whereas surrounding tissues were positive. When higher doses were used, tissues were culture positive more often than implants, with higher numbers of CFU. This was true for the different biomaterials tested, for both S. epidermidis strains, at different times, and for both mouse strains. S. epidermidis colocalized with host cells at a distance that was >10 cell layers from the biomaterial-tissue interface. We concluded that in mouse experimental BAI S. epidermidis peri-implant tissue colonization is more important than biofilm formation
U2 - https://doi.org/10.1128/IAI.01262-06
DO - https://doi.org/10.1128/IAI.01262-06
M3 - Article
C2 - 17158900
SN - 0019-9567
VL - 75
SP - 1129
EP - 1136
JO - Infection and immunity
JF - Infection and immunity
IS - 3
ER -