Peroxisomal trans-2-enoyl-CoA reductase is involved in phytol degradation

J. Gloerich; J. P. N. Ruiter; D. M. van den Brink; R. Ofman; S. Ferdinandusse; R. J. A. Wanders

doi:https://doi.org/10.1016/j.febslet.2006.03.011

Peroxisomal trans-2-enoyl-CoA reductase is involved in phytol degradation

J. Gloerich, J. P. N. Ruiter, D. M. van den Brink, R. Ofman, S. Ferdinandusse, R. J. A. Wanders

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Phytol is a naturally occurring precursor of phytanic acid. The last step in the conversion of phytol to phytanoyl-CoA is the reduction of phytenoyl-CoA mediated by an, as yet, unidentified enzyme. A candidate for this reaction is a previously described peroxisomal trans-2-enoyl-CoA reductase (TER). To investigate this, human TER was expressed in E. coli as an MBP-fusion protein. The purified recombinant protein was shown to have high reductase activity towards trans-phytenoyl-CoA, but not towards the peroxisomal beta-oxidation intermediates C24:1-CoA and pristenoyl-CoA. In conclusion, our results show that human TER is responsible for the reduction of phytenoyl-CoA to phytanoyl-CoA in peroxisomes

Original language	English
Pages (from-to)	2092-2096
Journal	FEBS letters
Volume	580
Issue number	8
DOIs	https://doi.org/10.1016/j.febslet.2006.03.011
Publication status	Published - 2006

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https://doi.org/10.1016/j.febslet.2006.03.011

Cite this

@article{458af0b61670494ca2604e73ee9f7693,

title = "Peroxisomal trans-2-enoyl-CoA reductase is involved in phytol degradation",

abstract = "Phytol is a naturally occurring precursor of phytanic acid. The last step in the conversion of phytol to phytanoyl-CoA is the reduction of phytenoyl-CoA mediated by an, as yet, unidentified enzyme. A candidate for this reaction is a previously described peroxisomal trans-2-enoyl-CoA reductase (TER). To investigate this, human TER was expressed in E. coli as an MBP-fusion protein. The purified recombinant protein was shown to have high reductase activity towards trans-phytenoyl-CoA, but not towards the peroxisomal beta-oxidation intermediates C24:1-CoA and pristenoyl-CoA. In conclusion, our results show that human TER is responsible for the reduction of phytenoyl-CoA to phytanoyl-CoA in peroxisomes",

author = "J. Gloerich and Ruiter, {J. P. N.} and {van den Brink}, {D. M.} and R. Ofman and S. Ferdinandusse and Wanders, {R. J. A.}",

year = "2006",

doi = "https://doi.org/10.1016/j.febslet.2006.03.011",

language = "English",

volume = "580",

pages = "2092--2096",

journal = "FEBS letters",

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TY - JOUR

T1 - Peroxisomal trans-2-enoyl-CoA reductase is involved in phytol degradation

AU - Gloerich, J.

AU - Ruiter, J. P. N.

AU - van den Brink, D. M.

AU - Ofman, R.

AU - Ferdinandusse, S.

AU - Wanders, R. J. A.

PY - 2006

Y1 - 2006

N2 - Phytol is a naturally occurring precursor of phytanic acid. The last step in the conversion of phytol to phytanoyl-CoA is the reduction of phytenoyl-CoA mediated by an, as yet, unidentified enzyme. A candidate for this reaction is a previously described peroxisomal trans-2-enoyl-CoA reductase (TER). To investigate this, human TER was expressed in E. coli as an MBP-fusion protein. The purified recombinant protein was shown to have high reductase activity towards trans-phytenoyl-CoA, but not towards the peroxisomal beta-oxidation intermediates C24:1-CoA and pristenoyl-CoA. In conclusion, our results show that human TER is responsible for the reduction of phytenoyl-CoA to phytanoyl-CoA in peroxisomes

AB - Phytol is a naturally occurring precursor of phytanic acid. The last step in the conversion of phytol to phytanoyl-CoA is the reduction of phytenoyl-CoA mediated by an, as yet, unidentified enzyme. A candidate for this reaction is a previously described peroxisomal trans-2-enoyl-CoA reductase (TER). To investigate this, human TER was expressed in E. coli as an MBP-fusion protein. The purified recombinant protein was shown to have high reductase activity towards trans-phytenoyl-CoA, but not towards the peroxisomal beta-oxidation intermediates C24:1-CoA and pristenoyl-CoA. In conclusion, our results show that human TER is responsible for the reduction of phytenoyl-CoA to phytanoyl-CoA in peroxisomes

U2 - https://doi.org/10.1016/j.febslet.2006.03.011

DO - https://doi.org/10.1016/j.febslet.2006.03.011

M3 - Article

C2 - 16546181

SN - 0014-5793

VL - 580

SP - 2092

EP - 2096

JO - FEBS letters

JF - FEBS letters

IS - 8

ER -