TY - JOUR
T1 - Phenotyping of left and right ventricular function in mouse models of compensated hypertrophy and heart failure with cardiac MRI
AU - van Nierop, Bastiaan J.
AU - van Assen, Hans C.
AU - van Deel, Elza D.
AU - Niesen, Leonie B. P.
AU - Duncker, Dirk J.
AU - Strijkers, Gustav J.
AU - Nicolay, Klaas
PY - 2013
Y1 - 2013
N2 - Left ventricular (LV) and right ventricular (RV) function have an important impact on symptom occurrence, disease progression and exercise tolerance in pressure overload-induced heart failure, but particularly RV functional changes are not well described in the relevant aortic banding mouse model. Therefore, we quantified time-dependent alterations in the ventricular morphology and function in two models of hypertrophy and heart failure and we studied the relationship between RV and LV function during the transition from hypertrophy to heart failure. MRI was used to quantify RV and LV function and morphology in healthy (n = 4) and sham operated (n = 3) C57BL/6 mice, and animals with a mild (n = 5) and a severe aortic constriction (n = 10). Mice subjected to a mild constriction showed increased LV mass (P <0.01) and depressed LV ejection fraction (EF) (P <0.05) as compared to controls, but had similar RVEF (P>0.05). Animals with a severe constriction progressively developed LV hypertrophy (P <0.001), depressed LVEF (P <0.001), followed by a declining RVEF (P <0.001) and the development of pulmonary remodeling, as compared to controls during a 10-week follow-up. Myocardial strain, as a measure for local cardiac function, decreased in mice with a severe constriction compared to controls (P <0.05). Relevant changes in mouse RV and LV function following an aortic constriction could be quantified using MRI. The well-controlled models described here open opportunities to assess the added value of new MRI techniques for the diagnosis of heart failure and to study the impact of new therapeutic strategies on disease progression and symptom occurrence
AB - Left ventricular (LV) and right ventricular (RV) function have an important impact on symptom occurrence, disease progression and exercise tolerance in pressure overload-induced heart failure, but particularly RV functional changes are not well described in the relevant aortic banding mouse model. Therefore, we quantified time-dependent alterations in the ventricular morphology and function in two models of hypertrophy and heart failure and we studied the relationship between RV and LV function during the transition from hypertrophy to heart failure. MRI was used to quantify RV and LV function and morphology in healthy (n = 4) and sham operated (n = 3) C57BL/6 mice, and animals with a mild (n = 5) and a severe aortic constriction (n = 10). Mice subjected to a mild constriction showed increased LV mass (P <0.01) and depressed LV ejection fraction (EF) (P <0.05) as compared to controls, but had similar RVEF (P>0.05). Animals with a severe constriction progressively developed LV hypertrophy (P <0.001), depressed LVEF (P <0.001), followed by a declining RVEF (P <0.001) and the development of pulmonary remodeling, as compared to controls during a 10-week follow-up. Myocardial strain, as a measure for local cardiac function, decreased in mice with a severe constriction compared to controls (P <0.05). Relevant changes in mouse RV and LV function following an aortic constriction could be quantified using MRI. The well-controlled models described here open opportunities to assess the added value of new MRI techniques for the diagnosis of heart failure and to study the impact of new therapeutic strategies on disease progression and symptom occurrence
U2 - https://doi.org/10.1371/journal.pone.0055424
DO - https://doi.org/10.1371/journal.pone.0055424
M3 - Article
C2 - 23383329
SN - 1932-6203
VL - 8
SP - e55424
JO - PLOS ONE
JF - PLOS ONE
IS - 2
ER -