TY - JOUR
T1 - Plasma citrulline during the first 48 h after onset of necrotizing enterocolitis in preterm infants
AU - Feenstra, Froukje A.
AU - Kuik, Sara J.
AU - Derikx, Joep P. M.
AU - Heiner-Fokkema, M. Rebecca
AU - Kooi, Elisabeth M. W.
AU - Bos, Arend F.
AU - Hulscher, Jan B. F.
N1 - Funding Information: We would like to acknowledge the nurses, medical staff of the NICU of Beatrix Children's Hospital in Groningen and the medical students, for helping with data collection and for creating the opportunity to carry out this study. We also greatly acknowledge E.Z. Jonkers, laboratory technician, for performing the citrulline-P analyses. Funding Information: These two studies were part of the research program of the postgraduate school for Behavioral and Cognitive Neurosciences, University of Groningen. S.J. Kuik was financially supported by a grant from the Junior Scientific Master Class of the University of Groningen, Groningen, the Netherlands. Additionally, we received grants from the NutsOhra Foundation, University Medical center Groningen ‘Doelmatigheidsfonds’ (intramural funding) and the Jan Kornelis de Cock Foundation. Publisher Copyright: © 2020 The Authors
PY - 2021/3
Y1 - 2021/3
N2 - Background: Levels of plasma citrulline (citrulline-P), a biomarker for enterocyte function, might be useful for the monitoring the course of necrotizing enterocolitis (NEC). Our aim was to evaluate whether citrulline-P levels during the first 48 h (h) after NEC onset were associated with need for surgery, survival, and intestinal recovery. Methods: In preterm infants with NEC (Bell's stage ≥2) we measured citrulline-P levels during the first 48 h after NEC onset. Categorizing the measurements into 0–8 h, 8–16 h, 16–24 h, 24–36 h, and 36–48 h, we determined the course of citrulline-P using linear regression analyses. Next, we analyzed whether citrulline-P levels measured at 0–24 h and 24–48 h differed between conservative and surgical treatment, survivors and nonsurvivors, and equal/below and above total group's median time to full enteral feeding (FEFt). Results: We included 48 infants, median gestational age 28.3 [IQR:26.0–31.4] weeks, birth weight 1200 [IQR:905–1524] grams. Citrulline-P levels decreased the first 48 h (B per time interval: -1.40 μmol, 95% CI, −2.73 to −0.07, p = 0.04). Citrulline-P was not associated with treatment, nor with survival. Citrulline-P at 0–24 h, but not 24–48 h, was higher in infants with FEFt ≤20 days than in infants with FEFt >20 days (20.7 [IQR:19.9–25.3] µmol/L (n = 13) vs. 11.1 [IQR:8.4–24.0] µmol/L (n = 11), p = 0.049), with a citrulline-P cut-off value of 12.3 μmol/L. Conclusion: Citrulline-P levels decreased the first 48 h after NEC onset, suggesting on-going intestinal injury. In survivors, measuring citrulline-P in the first 24 h after NEC onset may provide an indication for intestinal recovery rate.
AB - Background: Levels of plasma citrulline (citrulline-P), a biomarker for enterocyte function, might be useful for the monitoring the course of necrotizing enterocolitis (NEC). Our aim was to evaluate whether citrulline-P levels during the first 48 h (h) after NEC onset were associated with need for surgery, survival, and intestinal recovery. Methods: In preterm infants with NEC (Bell's stage ≥2) we measured citrulline-P levels during the first 48 h after NEC onset. Categorizing the measurements into 0–8 h, 8–16 h, 16–24 h, 24–36 h, and 36–48 h, we determined the course of citrulline-P using linear regression analyses. Next, we analyzed whether citrulline-P levels measured at 0–24 h and 24–48 h differed between conservative and surgical treatment, survivors and nonsurvivors, and equal/below and above total group's median time to full enteral feeding (FEFt). Results: We included 48 infants, median gestational age 28.3 [IQR:26.0–31.4] weeks, birth weight 1200 [IQR:905–1524] grams. Citrulline-P levels decreased the first 48 h (B per time interval: -1.40 μmol, 95% CI, −2.73 to −0.07, p = 0.04). Citrulline-P was not associated with treatment, nor with survival. Citrulline-P at 0–24 h, but not 24–48 h, was higher in infants with FEFt ≤20 days than in infants with FEFt >20 days (20.7 [IQR:19.9–25.3] µmol/L (n = 13) vs. 11.1 [IQR:8.4–24.0] µmol/L (n = 11), p = 0.049), with a citrulline-P cut-off value of 12.3 μmol/L. Conclusion: Citrulline-P levels decreased the first 48 h after NEC onset, suggesting on-going intestinal injury. In survivors, measuring citrulline-P in the first 24 h after NEC onset may provide an indication for intestinal recovery rate.
KW - Citrulline
KW - Full enteral feeding
KW - Necrotizing enterocolitis
KW - Preterm infants
KW - Survival
KW - Treatment
UR - http://www.scopus.com/inward/record.url?scp=85097084800&partnerID=8YFLogxK
U2 - https://doi.org/10.1016/j.jpedsurg.2020.11.020
DO - https://doi.org/10.1016/j.jpedsurg.2020.11.020
M3 - Article
C2 - 33276973
SN - 0022-3468
VL - 56
SP - 476
EP - 482
JO - Journal of Pediatric Surgery
JF - Journal of Pediatric Surgery
IS - 3
ER -