TY - JOUR
T1 - Plasma extracellular vesicle proteins are associated with stress-induced myocardial ischemia in women presenting with chest pain
AU - Dekker, Mirthe
AU - Waissi, Farahnaz
AU - van Bennekom, Joelle
AU - Silvis, Max J. M.
AU - Timmerman, Nathalie
AU - Bank, Ingrid E. M.
AU - Walter, Joan E.
AU - Mueller, Christian
AU - Schoneveld, A. H.
AU - Schiffelers, Raymond M.
AU - Pasterkamp, Gerard
AU - Grobbee, Diederick E.
AU - de Winter, Robbert J.
AU - Mosterd, A.
AU - de Kleijn, Dominique P. V.
AU - Timmers, Leo
PY - 2020/12/1
Y1 - 2020/12/1
N2 - Diagnosing stable ischemic heart disease (IHD) is challenging, especially in females. Currently, no blood test is available. Plasma extracellular vesicles (EV) are emerging as potential biomarker source. We therefore aimed to identify stress induced ischemia due to stable IHD with plasma extracellular vesicle protein levels in chest pain patients. We analyzed 450 patients suspected for stable IHD who were referred for 82Rb PET/CT in the outpatient clinic. Blood samples were collected before PET/CT and plasma EVs were isolated in 3 plasma subfractions named: TEX, HDL, LDL. In total 6 proteins were quantified in each of these subfractions using immuno-bead assays. CD14 and CystatinC protein levels were independent significant predictors of stress-induced ischemia in the LDL and the HDL subfraction and SerpinC1 and SerpinG1 protein levels in the HDL fraction. Subgroup-analysis on sex revealed that these associations were completely attributed to the associations in women. None of the significant EV proteins remained significant in men. Plasma EV proteins levels are associated with the presence of stable IHD in females presenting with chest pain. This finding, if confirmed in larger cohort studies could be a crucial step in improving diagnostic assessment of women with suspected IHD.
AB - Diagnosing stable ischemic heart disease (IHD) is challenging, especially in females. Currently, no blood test is available. Plasma extracellular vesicles (EV) are emerging as potential biomarker source. We therefore aimed to identify stress induced ischemia due to stable IHD with plasma extracellular vesicle protein levels in chest pain patients. We analyzed 450 patients suspected for stable IHD who were referred for 82Rb PET/CT in the outpatient clinic. Blood samples were collected before PET/CT and plasma EVs were isolated in 3 plasma subfractions named: TEX, HDL, LDL. In total 6 proteins were quantified in each of these subfractions using immuno-bead assays. CD14 and CystatinC protein levels were independent significant predictors of stress-induced ischemia in the LDL and the HDL subfraction and SerpinC1 and SerpinG1 protein levels in the HDL fraction. Subgroup-analysis on sex revealed that these associations were completely attributed to the associations in women. None of the significant EV proteins remained significant in men. Plasma EV proteins levels are associated with the presence of stable IHD in females presenting with chest pain. This finding, if confirmed in larger cohort studies could be a crucial step in improving diagnostic assessment of women with suspected IHD.
UR - http://www.scopus.com/inward/record.url?scp=85088393726&partnerID=8YFLogxK
U2 - https://doi.org/10.1038/s41598-020-69297-0
DO - https://doi.org/10.1038/s41598-020-69297-0
M3 - Article
C2 - 32704130
SN - 2045-2322
VL - 10
JO - Scientific reports
JF - Scientific reports
IS - 1
M1 - 12257
ER -