Polar body array CGH for prediction of the status of the corresponding oocyte. Part II: technical aspects

M. Cristina Magli; Markus Montag; Maria Köster; Luigi Muzi; Joep Geraedts; John Collins; Veerle Goossens; Alan H. Handyside; Joyce Harper; Sjoerd Repping; Andreas Schmutzler; Katerina Vesela; Luca Gianaroli

doi:https://doi.org/10.1093/humrep/der295

Polar body array CGH for prediction of the status of the corresponding oocyte. Part II: technical aspects

M. Cristina Magli, Markus Montag, Maria Köster, Luigi Muzi, Joep Geraedts, John Collins, Veerle Goossens, Alan H. Handyside, Joyce Harper, Sjoerd Repping, Andreas Schmutzler, Katerina Vesela, Luca Gianaroli

Research output: Contribution to journal › Editorial › Academic › peer-review

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Abstract

The purpose of this study was to assess the technical aspects related to polar body (PB) biopsy, which might have an influence on the results of the microarray comparative genomic hybridization analysis. Furthermore, a comparison was made between two biopsy methods (mechanical and laser). Biopsy of the first and second PB (PB1 and PB2) was performed by mechanical- or laser-assisted biopsy in two different IVF centres. PBs were separately amplified by whole genome amplification. The method of biopsy, mechanical or laser had no influence on the proportion of successfully biopsied oocytes. Especially, for the PB2, the timing of biopsy after ICSI was directly correlated to amplification efficiency. Special care has to be taken with respect to the timing of biopsy of the PB2. Mechanical- and laser-assisted biopsy give the same performance in terms of diagnostic efficiency

Original language	English
Pages (from-to)	3181-3185
Journal	Human reproduction (Oxford, England)
Volume	26
Issue number	11
DOIs	https://doi.org/10.1093/humrep/der295
Publication status	Published - 2011

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Magli, M. C., Montag, M., Köster, M., Muzi, L., Geraedts, J., Collins, J., Goossens, V., Handyside, A. H., Harper, J., Repping, S., Schmutzler, A., Vesela, K., & Gianaroli, L. (2011). Polar body array CGH for prediction of the status of the corresponding oocyte. Part II: technical aspects. Human reproduction (Oxford, England), 26(11), 3181-3185. https://doi.org/10.1093/humrep/der295

@article{d22c30c8444d4698980e3d7111a7f686,

title = "Polar body array CGH for prediction of the status of the corresponding oocyte. Part II: technical aspects",

abstract = "The purpose of this study was to assess the technical aspects related to polar body (PB) biopsy, which might have an influence on the results of the microarray comparative genomic hybridization analysis. Furthermore, a comparison was made between two biopsy methods (mechanical and laser). Biopsy of the first and second PB (PB1 and PB2) was performed by mechanical- or laser-assisted biopsy in two different IVF centres. PBs were separately amplified by whole genome amplification. The method of biopsy, mechanical or laser had no influence on the proportion of successfully biopsied oocytes. Especially, for the PB2, the timing of biopsy after ICSI was directly correlated to amplification efficiency. Special care has to be taken with respect to the timing of biopsy of the PB2. Mechanical- and laser-assisted biopsy give the same performance in terms of diagnostic efficiency",

author = "Magli, {M. Cristina} and Markus Montag and Maria K{\"o}ster and Luigi Muzi and Joep Geraedts and John Collins and Veerle Goossens and Handyside, {Alan H.} and Joyce Harper and Sjoerd Repping and Andreas Schmutzler and Katerina Vesela and Luca Gianaroli",

year = "2011",

doi = "https://doi.org/10.1093/humrep/der295",

language = "English",

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pages = "3181--3185",

journal = "Human reproduction (Oxford, England)",

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Magli, MC, Montag, M, Köster, M, Muzi, L, Geraedts, J, Collins, J, Goossens, V, Handyside, AH, Harper, J, Repping, S, Schmutzler, A, Vesela, K & Gianaroli, L 2011, 'Polar body array CGH for prediction of the status of the corresponding oocyte. Part II: technical aspects', Human reproduction (Oxford, England), vol. 26, no. 11, pp. 3181-3185. https://doi.org/10.1093/humrep/der295

TY - JOUR

T1 - Polar body array CGH for prediction of the status of the corresponding oocyte. Part II: technical aspects

AU - Magli, M. Cristina

AU - Montag, Markus

AU - Köster, Maria

AU - Muzi, Luigi

AU - Geraedts, Joep

AU - Collins, John

AU - Goossens, Veerle

AU - Handyside, Alan H.

AU - Harper, Joyce

AU - Repping, Sjoerd

AU - Schmutzler, Andreas

AU - Vesela, Katerina

AU - Gianaroli, Luca

PY - 2011

Y1 - 2011

N2 - The purpose of this study was to assess the technical aspects related to polar body (PB) biopsy, which might have an influence on the results of the microarray comparative genomic hybridization analysis. Furthermore, a comparison was made between two biopsy methods (mechanical and laser). Biopsy of the first and second PB (PB1 and PB2) was performed by mechanical- or laser-assisted biopsy in two different IVF centres. PBs were separately amplified by whole genome amplification. The method of biopsy, mechanical or laser had no influence on the proportion of successfully biopsied oocytes. Especially, for the PB2, the timing of biopsy after ICSI was directly correlated to amplification efficiency. Special care has to be taken with respect to the timing of biopsy of the PB2. Mechanical- and laser-assisted biopsy give the same performance in terms of diagnostic efficiency

AB - The purpose of this study was to assess the technical aspects related to polar body (PB) biopsy, which might have an influence on the results of the microarray comparative genomic hybridization analysis. Furthermore, a comparison was made between two biopsy methods (mechanical and laser). Biopsy of the first and second PB (PB1 and PB2) was performed by mechanical- or laser-assisted biopsy in two different IVF centres. PBs were separately amplified by whole genome amplification. The method of biopsy, mechanical or laser had no influence on the proportion of successfully biopsied oocytes. Especially, for the PB2, the timing of biopsy after ICSI was directly correlated to amplification efficiency. Special care has to be taken with respect to the timing of biopsy of the PB2. Mechanical- and laser-assisted biopsy give the same performance in terms of diagnostic efficiency

U2 - https://doi.org/10.1093/humrep/der295

DO - https://doi.org/10.1093/humrep/der295

M3 - Editorial

C2 - 21908464

SN - 0268-1161

VL - 26

SP - 3181

EP - 3185

JO - Human reproduction (Oxford, England)

JF - Human reproduction (Oxford, England)

IS - 11

ER -