Porcine alpha-1-antitrypsin (PI): cDNA sequence, polymorphism and assignment to chromosome 7q2.4- > q2.6

A. L. Archibald; S. Couperwhite; C. H.M. Mellink; Y. Lahbib-Mansais; J. Gellin

doi:https://doi.org/10.1111/j.1365-2052.1996.tb00472.x

Porcine alpha-1-antitrypsin (PI): cDNA sequence, polymorphism and assignment to chromosome 7q2.4- > q2.6

A. L. Archibald, S. Couperwhite, C. H.M. Mellink, Y. Lahbib-Mansais, J. Gellin

Human Genetics (AMC)

Research output: Contribution to journal › Article › Academic › peer-review

8 Citations (Scopus)

Abstract

A cDNA clone encoding the complete coding sequence for porcine alpha-1-antitrypsin (or alprotease inhibitor, PI) was isolated and its DNA sequence determined. The cDNA is assumed to encode alpha-1-antitrypsin on the basis of its sequence similarity to the corresponding cDNAs for human, baboon, rat, mouse, sheep and cow. The porcine cDNA clone was used in conjunction with BamHI, KpnI, MspI, SacI, TaqI and XbaI to develop restriction fragment length polymorphism-based genetic markers for linkage mapping in pigs. The cDNA has also been used to map the porcine PI locus to chromosome 7q2.4- > q2.6 by radioactive in situ hybridization. Thus, the PI locus has been added to the developing physical and genetic maps of the porcine genome.

Original language	English
Pages (from-to)	85-90
Number of pages	6
Journal	Animal genetics
Volume	27
Issue number	2
DOIs	https://doi.org/10.1111/j.1365-2052.1996.tb00472.x
Publication status	Published - Apr 1996

Keywords

Alpha-1-antitrypsin
Chromosome 7
Gene mapping
PI
PiGMaP
Pigs

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title = "Porcine alpha-1-antitrypsin (PI): cDNA sequence, polymorphism and assignment to chromosome 7q2.4- > q2.6",

abstract = "A cDNA clone encoding the complete coding sequence for porcine alpha-1-antitrypsin (or alprotease inhibitor, PI) was isolated and its DNA sequence determined. The cDNA is assumed to encode alpha-1-antitrypsin on the basis of its sequence similarity to the corresponding cDNAs for human, baboon, rat, mouse, sheep and cow. The porcine cDNA clone was used in conjunction with BamHI, KpnI, MspI, SacI, TaqI and XbaI to develop restriction fragment length polymorphism-based genetic markers for linkage mapping in pigs. The cDNA has also been used to map the porcine PI locus to chromosome 7q2.4- > q2.6 by radioactive in situ hybridization. Thus, the PI locus has been added to the developing physical and genetic maps of the porcine genome.",

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AU - Mellink, C. H.M.

AU - Lahbib-Mansais, Y.

AU - Gellin, J.

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KW - Alpha-1-antitrypsin

KW - Chromosome 7

KW - Gene mapping

KW - PI

KW - PiGMaP

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Porcine alpha-1-antitrypsin (PI): cDNA sequence, polymorphism and assignment to chromosome 7q2.4- > q2.6

Abstract

Keywords

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