Preparation and specificity testing of a rabbit anti-human thymocyte serum (with 1 colour plate)

A. Brutel de la Rivière; P. R. Verhoef-Karssen; M. H. van Oers; R. Schoorl; T. M. Feltkamp-Vroom; A. E. von dem Borne; W. P. Zeijlemaker

doi:https://doi.org/10.1111/j.1423-0410.1977.tb04471.x

Preparation and specificity testing of a rabbit anti-human thymocyte serum (with 1 colour plate)

A. Brutel de la Rivière, P. R. Verhoef-Karssen, M. H. van Oers, R. Schoorl, T. M. Feltkamp-Vroom, A. E. von dem Borne, W. P. Zeijlemaker

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Abstract

The preparation of a specific anti-T cell serum, applicable in the indirect immunofluorescence technique on cell suspensions, smears of peripheral blood and bone marrow and on tissue sections is described. Rabbits were immunized with thymocytes; after removal of antibodies against species-specific antigens and antigens common to leucocytes by absorptions with red cells and granulocytes, specificity for thymocytes was obtained by repeated absorptions with CLL cells, used as B cell equivalents. Subsequently, the IgG fraction of the absorbed antiserum was isolated. This antibody preparation was tested with various types of blood cells as well as with cell suspensions depleted or enriched in T cells. For the study of tissue sections it had to be absorbed with liver powder. When studying lymphatic tissue it was found to stain only thymus-dependent areas in the specimens tested

Original language	English
Pages (from-to)	257-265
Journal	Vox sanguinis
Volume	33
Issue number	5
DOIs	https://doi.org/10.1111/j.1423-0410.1977.tb04471.x
Publication status	Published - 1977

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https://doi.org/10.1111/j.1423-0410.1977.tb04471.x

Cite this

@article{33017a98bd3f41aa845bf7e223e248fd,

title = "Preparation and specificity testing of a rabbit anti-human thymocyte serum (with 1 colour plate)",

abstract = "The preparation of a specific anti-T cell serum, applicable in the indirect immunofluorescence technique on cell suspensions, smears of peripheral blood and bone marrow and on tissue sections is described. Rabbits were immunized with thymocytes; after removal of antibodies against species-specific antigens and antigens common to leucocytes by absorptions with red cells and granulocytes, specificity for thymocytes was obtained by repeated absorptions with CLL cells, used as B cell equivalents. Subsequently, the IgG fraction of the absorbed antiserum was isolated. This antibody preparation was tested with various types of blood cells as well as with cell suspensions depleted or enriched in T cells. For the study of tissue sections it had to be absorbed with liver powder. When studying lymphatic tissue it was found to stain only thymus-dependent areas in the specimens tested",

author = "{Brutel de la Rivi{\`e}re}, A. and Verhoef-Karssen, {P. R.} and {van Oers}, {M. H.} and R. Schoorl and Feltkamp-Vroom, {T. M.} and {von dem Borne}, {A. E.} and Zeijlemaker, {W. P.}",

year = "1977",

doi = "https://doi.org/10.1111/j.1423-0410.1977.tb04471.x",

language = "English",

volume = "33",

pages = "257--265",

journal = "Vox sanguinis",

issn = "0042-9007",

publisher = "Wiley-Blackwell",

number = "5",

}

TY - JOUR

T1 - Preparation and specificity testing of a rabbit anti-human thymocyte serum (with 1 colour plate)

AU - Brutel de la Rivière, A.

AU - Verhoef-Karssen, P. R.

AU - van Oers, M. H.

AU - Schoorl, R.

AU - Feltkamp-Vroom, T. M.

AU - von dem Borne, A. E.

AU - Zeijlemaker, W. P.

PY - 1977

Y1 - 1977

N2 - The preparation of a specific anti-T cell serum, applicable in the indirect immunofluorescence technique on cell suspensions, smears of peripheral blood and bone marrow and on tissue sections is described. Rabbits were immunized with thymocytes; after removal of antibodies against species-specific antigens and antigens common to leucocytes by absorptions with red cells and granulocytes, specificity for thymocytes was obtained by repeated absorptions with CLL cells, used as B cell equivalents. Subsequently, the IgG fraction of the absorbed antiserum was isolated. This antibody preparation was tested with various types of blood cells as well as with cell suspensions depleted or enriched in T cells. For the study of tissue sections it had to be absorbed with liver powder. When studying lymphatic tissue it was found to stain only thymus-dependent areas in the specimens tested

AB - The preparation of a specific anti-T cell serum, applicable in the indirect immunofluorescence technique on cell suspensions, smears of peripheral blood and bone marrow and on tissue sections is described. Rabbits were immunized with thymocytes; after removal of antibodies against species-specific antigens and antigens common to leucocytes by absorptions with red cells and granulocytes, specificity for thymocytes was obtained by repeated absorptions with CLL cells, used as B cell equivalents. Subsequently, the IgG fraction of the absorbed antiserum was isolated. This antibody preparation was tested with various types of blood cells as well as with cell suspensions depleted or enriched in T cells. For the study of tissue sections it had to be absorbed with liver powder. When studying lymphatic tissue it was found to stain only thymus-dependent areas in the specimens tested

U2 - https://doi.org/10.1111/j.1423-0410.1977.tb04471.x

DO - https://doi.org/10.1111/j.1423-0410.1977.tb04471.x

M3 - Article

C2 - 72451

SN - 0042-9007

VL - 33

SP - 257

EP - 265

JO - Vox sanguinis

JF - Vox sanguinis

IS - 5

ER -