Pretargeted PET Imaging of trans-Cyclooctene-Modified Porous Silicon Nanoparticles

Outi Keinänen; Ermei M. Mäkilä; Rici Lindgren; Helena Virtanen; Heidi Liljenbäck; Vesa Oikonen; Mirkka Sarparanta; Carla Molthoff; Albert D. Windhorst; Anne Roivainen; Jarno J. Salonen; Anu J. Airaksinen

doi:https://doi.org/10.1021/acsomega.6b00269

Pretargeted PET Imaging of trans-Cyclooctene-Modified Porous Silicon Nanoparticles

Outi Keinänen, Ermei M. Mäkilä, Rici Lindgren, Helena Virtanen, Heidi Liljenbäck, Vesa Oikonen, Mirkka Sarparanta, Carla Molthoff, Albert D. Windhorst, Anne Roivainen, Jarno J. Salonen, Anu J. Airaksinen

Research output: Contribution to journal › Article › Academic › peer-review

48 Citations (Scopus)

Abstract

Pretargeted positron emission tomography (PET) imaging based on bioorthogonal chemical reactions has proven its potential in immunoimaging. It may also have great potential in nanotheranostic applications. Here, we report the first successful pretargeted PET imaging of trans-cyclooctene-modified mesoporous silicon nanoparticles, using ¹⁸F-labeled tetrazine as a tracer. The inverse electron-demand Diels-Alder cycloaddition (IEDDA) reaction was fast, resulting in high radioactivity accumulation in the expected organs within 10 min after the administration of the tracer. The highest target-to-background ratio was achieved 120 min after the tracer injection. A clear correlation between the efficiency of the in vivo IEDDA labeling reaction and the injected amount of the tracer was observed. The radioactivity accumulation decreased with the increased amount of the co-injected carrier, indicating saturation in the reaction sites. This finding was supported by the in vitro results. Our study suggests that pretargeted imaging has excellent potential in nanotheranostic PET imaging when using high-specific-activity tracers.

Original language	English
Pages (from-to)	62-69
Number of pages	8
Journal	ACS Omega
Volume	2
Issue number	1
DOIs	https://doi.org/10.1021/acsomega.6b00269
Publication status	Published - 31 Jan 2017

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title = "Pretargeted PET Imaging of trans-Cyclooctene-Modified Porous Silicon Nanoparticles",

abstract = "Pretargeted positron emission tomography (PET) imaging based on bioorthogonal chemical reactions has proven its potential in immunoimaging. It may also have great potential in nanotheranostic applications. Here, we report the first successful pretargeted PET imaging of trans-cyclooctene-modified mesoporous silicon nanoparticles, using 18F-labeled tetrazine as a tracer. The inverse electron-demand Diels-Alder cycloaddition (IEDDA) reaction was fast, resulting in high radioactivity accumulation in the expected organs within 10 min after the administration of the tracer. The highest target-to-background ratio was achieved 120 min after the tracer injection. A clear correlation between the efficiency of the in vivo IEDDA labeling reaction and the injected amount of the tracer was observed. The radioactivity accumulation decreased with the increased amount of the co-injected carrier, indicating saturation in the reaction sites. This finding was supported by the in vitro results. Our study suggests that pretargeted imaging has excellent potential in nanotheranostic PET imaging when using high-specific-activity tracers.",

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AU - Keinänen, Outi

AU - Mäkilä, Ermei M.

AU - Lindgren, Rici

AU - Virtanen, Helena

AU - Liljenbäck, Heidi

AU - Oikonen, Vesa

AU - Sarparanta, Mirkka

AU - Molthoff, Carla

AU - Windhorst, Albert D.

AU - Roivainen, Anne

AU - Salonen, Jarno J.

AU - Airaksinen, Anu J.

PY - 2017/1/31

Y1 - 2017/1/31

N2 - Pretargeted positron emission tomography (PET) imaging based on bioorthogonal chemical reactions has proven its potential in immunoimaging. It may also have great potential in nanotheranostic applications. Here, we report the first successful pretargeted PET imaging of trans-cyclooctene-modified mesoporous silicon nanoparticles, using 18F-labeled tetrazine as a tracer. The inverse electron-demand Diels-Alder cycloaddition (IEDDA) reaction was fast, resulting in high radioactivity accumulation in the expected organs within 10 min after the administration of the tracer. The highest target-to-background ratio was achieved 120 min after the tracer injection. A clear correlation between the efficiency of the in vivo IEDDA labeling reaction and the injected amount of the tracer was observed. The radioactivity accumulation decreased with the increased amount of the co-injected carrier, indicating saturation in the reaction sites. This finding was supported by the in vitro results. Our study suggests that pretargeted imaging has excellent potential in nanotheranostic PET imaging when using high-specific-activity tracers.

AB - Pretargeted positron emission tomography (PET) imaging based on bioorthogonal chemical reactions has proven its potential in immunoimaging. It may also have great potential in nanotheranostic applications. Here, we report the first successful pretargeted PET imaging of trans-cyclooctene-modified mesoporous silicon nanoparticles, using 18F-labeled tetrazine as a tracer. The inverse electron-demand Diels-Alder cycloaddition (IEDDA) reaction was fast, resulting in high radioactivity accumulation in the expected organs within 10 min after the administration of the tracer. The highest target-to-background ratio was achieved 120 min after the tracer injection. A clear correlation between the efficiency of the in vivo IEDDA labeling reaction and the injected amount of the tracer was observed. The radioactivity accumulation decreased with the increased amount of the co-injected carrier, indicating saturation in the reaction sites. This finding was supported by the in vitro results. Our study suggests that pretargeted imaging has excellent potential in nanotheranostic PET imaging when using high-specific-activity tracers.

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Pretargeted PET Imaging of trans-Cyclooctene-Modified Porous Silicon Nanoparticles

Abstract

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