TY - JOUR
T1 - Probing Lipid Coating Dynamics of Quantum Dot Core Micelles via Forster Resonance Energy Transfer
AU - Zhao, Yiming
AU - Schapotschnikow, Philipp
AU - Skajaa, Torjus
AU - Vlugt, Thijs J. H.
AU - Mulder, Willem J. M.
AU - de Mello Donegá, Celso
AU - Meijerink, Andries
PY - 2014
Y1 - 2014
N2 - Lipid coated nanocrystal assemblies are among the most extensively investigated nanoparticle platforms for biomedical imaging and therapeutic purposes. However, very few efforts have been addressed to the lipid coating exchange dynamics in such systems, which is key to our understanding of the nanoparticles' coating stability and their interactions with the environment. Here, we apply the Forster resonance energy transfer (FRET) from quantum dot (QD) core to Cy5.5 dye labeled lipids at the surface to monitor the lipid exchange dynamics in situ and to study its dependence on concentration, temperature and solvent. A kinetic model is developed to describe the experimental data, allowing the rate constants and the activation energy for lipid exchange to be determined. The activation energy for lipid exchange on QD micelles is 155 kJ/mol in saline environment and 130 kJ/mol in pure water. The findings presented here provide basic knowledge on these self-assembled structures and contribute to understanding their performance and to further design of nanomedicine
AB - Lipid coated nanocrystal assemblies are among the most extensively investigated nanoparticle platforms for biomedical imaging and therapeutic purposes. However, very few efforts have been addressed to the lipid coating exchange dynamics in such systems, which is key to our understanding of the nanoparticles' coating stability and their interactions with the environment. Here, we apply the Forster resonance energy transfer (FRET) from quantum dot (QD) core to Cy5.5 dye labeled lipids at the surface to monitor the lipid exchange dynamics in situ and to study its dependence on concentration, temperature and solvent. A kinetic model is developed to describe the experimental data, allowing the rate constants and the activation energy for lipid exchange to be determined. The activation energy for lipid exchange on QD micelles is 155 kJ/mol in saline environment and 130 kJ/mol in pure water. The findings presented here provide basic knowledge on these self-assembled structures and contribute to understanding their performance and to further design of nanomedicine
U2 - https://doi.org/10.1002/smll.201301962
DO - https://doi.org/10.1002/smll.201301962
M3 - Article
C2 - 24343988
SN - 1613-6810
VL - 10
SP - 1163
EP - 1170
JO - Small (Weinheim an der Bergstrasse, Germany)
JF - Small (Weinheim an der Bergstrasse, Germany)
IS - 6
ER -