TY - JOUR
T1 - Processing methods of donor human milk evaluated by a blood plasma clotting assay
AU - Hu, Yong
AU - Kontopodi, Eva
AU - Mank, Elise
AU - van den Akker, Chris H. P.
AU - van Goudoever, Johannes B.
AU - Hettinga, Kasper
AU - van Elburg, Ruurd M.
AU - Thaler, Johannes
AU - Nieuwland, Rienk
N1 - Funding Information: Y.H. was supported by a scholarship from the China Scholarship Council (CSC). J. T. was supported by an unrestricted travel grant from the International Society on Thrombosis and Haemostasis. Publisher Copyright: © 2022 The Authors
PY - 2022/3/1
Y1 - 2022/3/1
N2 - Donor human milk is the first alternative for preterm infants when mother's own milk is not available. Most available human milk banking guidelines recommend classical holder pasteurization to ensure safety by eliminating potential infectious microorganisms. Processing by heat treatment, however, negatively affects functionality and availability of bioactive components naturally present in human milk. Here we compared the effect of five different processing methods on the ability of human milk to induce blood plasma clotting, which was recently described as a bioactive function present in human milk. From thirty lactating women, milk samples were collected, and all milk samples were subjected to holder pasteurization (30 min at 62.5 °C), high-temperature-short-time pasteurization (15 s at 72 °C), high-pressure processing (5 min at 500 MPa), ultraviolet-C irradiation (4863 J/L), or thermo-ultrasonication (6 min at 60 W, at 40 °C). All methods significantly reduced the ability of milk to trigger blood plasma clotting compared to untreated milk, but ultraviolet-C irradiation and high-pressure processing were best at preserving this activity. Taken together, measuring the ability of milk to induce blood plasma clotting may offer a new tool to monitor the effect of human milk processing.
AB - Donor human milk is the first alternative for preterm infants when mother's own milk is not available. Most available human milk banking guidelines recommend classical holder pasteurization to ensure safety by eliminating potential infectious microorganisms. Processing by heat treatment, however, negatively affects functionality and availability of bioactive components naturally present in human milk. Here we compared the effect of five different processing methods on the ability of human milk to induce blood plasma clotting, which was recently described as a bioactive function present in human milk. From thirty lactating women, milk samples were collected, and all milk samples were subjected to holder pasteurization (30 min at 62.5 °C), high-temperature-short-time pasteurization (15 s at 72 °C), high-pressure processing (5 min at 500 MPa), ultraviolet-C irradiation (4863 J/L), or thermo-ultrasonication (6 min at 60 W, at 40 °C). All methods significantly reduced the ability of milk to trigger blood plasma clotting compared to untreated milk, but ultraviolet-C irradiation and high-pressure processing were best at preserving this activity. Taken together, measuring the ability of milk to induce blood plasma clotting may offer a new tool to monitor the effect of human milk processing.
KW - Blood plasma clotting
KW - High-pressure processing
KW - High-temperature-short-time
KW - Pasteurization
KW - Thermo-ultrasonication
KW - Ultraviolet-C irradiation
UR - http://www.scopus.com/inward/record.url?scp=85123287634&partnerID=8YFLogxK
U2 - https://doi.org/10.1016/j.ifset.2022.102938
DO - https://doi.org/10.1016/j.ifset.2022.102938
M3 - Article
SN - 1466-8564
VL - 76
JO - Innovative Food Science and Emerging Technologies
JF - Innovative Food Science and Emerging Technologies
IS - March
M1 - 102938
ER -