Protocol to analyze transmigration of human cytotoxic T cells under physiological flow conditions in vitro

Rouven Schoppmeyer; Jaap D. van Buul

doi:https://doi.org/10.1016/j.xpro.2022.101509

Protocol to analyze transmigration of human cytotoxic T cells under physiological flow conditions in vitro

Rouven Schoppmeyer, Jaap D. van Buul

Research output: Contribution to journal › Article › Academic › peer-review

2 Citations (Scopus)

Abstract

This protocol presents an assay for transmigration analysis of human cytotoxic T cells (CTL) under physiological flow in vitro. We describe detailed analysis steps of human CTL behavior, from adhesion to diapedesis, using live cell imaging which cannot be achieved by in vivo imaging. The flow system is made of 2D plastic surfaces covered by an endothelial monolayer limiting the system but allows for quantitative analysis of CTL behavior with high modifiability. For complete details on the use and execution of this protocol, please refer to Schoppmeyer et al. (2022).

Original language	English
Article number	101509
Journal	STAR Protocols
Volume	3
Issue number	3
DOIs	https://doi.org/10.1016/j.xpro.2022.101509
Publication status	Published - 16 Sept 2022

Keywords

Cell Biology
Immunology
Microscopy

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https://doi.org/10.1016/j.xpro.2022.101509

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title = "Protocol to analyze transmigration of human cytotoxic T cells under physiological flow conditions in vitro",

abstract = "This protocol presents an assay for transmigration analysis of human cytotoxic T cells (CTL) under physiological flow in vitro. We describe detailed analysis steps of human CTL behavior, from adhesion to diapedesis, using live cell imaging which cannot be achieved by in vivo imaging. The flow system is made of 2D plastic surfaces covered by an endothelial monolayer limiting the system but allows for quantitative analysis of CTL behavior with high modifiability. For complete details on the use and execution of this protocol, please refer to Schoppmeyer et al. (2022).",

keywords = "Cell Biology, Immunology, Microscopy",

author = "Rouven Schoppmeyer and {van Buul}, {Jaap D.}",

note = "Funding Information: This work was supported by ZonMW NWO Vici grant #91819632 (R.S. and J.D.v.B.). Publisher Copyright: {\textcopyright} 2022 The Author(s)",

year = "2022",

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AU - van Buul, Jaap D.

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N2 - This protocol presents an assay for transmigration analysis of human cytotoxic T cells (CTL) under physiological flow in vitro. We describe detailed analysis steps of human CTL behavior, from adhesion to diapedesis, using live cell imaging which cannot be achieved by in vivo imaging. The flow system is made of 2D plastic surfaces covered by an endothelial monolayer limiting the system but allows for quantitative analysis of CTL behavior with high modifiability. For complete details on the use and execution of this protocol, please refer to Schoppmeyer et al. (2022).

AB - This protocol presents an assay for transmigration analysis of human cytotoxic T cells (CTL) under physiological flow in vitro. We describe detailed analysis steps of human CTL behavior, from adhesion to diapedesis, using live cell imaging which cannot be achieved by in vivo imaging. The flow system is made of 2D plastic surfaces covered by an endothelial monolayer limiting the system but allows for quantitative analysis of CTL behavior with high modifiability. For complete details on the use and execution of this protocol, please refer to Schoppmeyer et al. (2022).

KW - Cell Biology

KW - Immunology

KW - Microscopy

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JO - STAR Protocols

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ER -

Protocol to analyze transmigration of human cytotoxic T cells under physiological flow conditions in vitro

Abstract

Keywords

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